Viscotek A-Columns are designed for the separation of water-soluble polymers and oligomers by size exclusion chromatography (SEC). The packing material is a porous poly hydroxymethacrylate polymer. There are 8 types of columns for different molecular weight ranges.
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Column size: |
Analytical columns
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300mm L×7.8mm ID |
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Guard column |
50mm L×6.0mm ID (AGuard)
50mm L×8.0mm ID (A7Guard)
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Packing material
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Porous poly hydroxymethacrylate polymer |
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Connecting screws
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Internally-threaded type, No.10 32 UNF |
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Column material
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Stainless steel type SUS-316 |
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Shipping solvent
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0.02% sodium azide aqueous solution (water with antibacterial)
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Max pressure |
(A2000-A3000) 5.0MPa (A4000-A6000M) 3.0MPa
(A7000) 0.5MPa
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Max flow rate |
(A2000-A6000M) 1.2mL/min (A7000) 1.5mL/min
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Temperature Range |
(A2000-A6000M) 4 - 70 ℃ (A7000) 4- 60℃
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pH range |
3 -10
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Usable salt concentration |
< 0.5 M
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Organic solvents |
refer to Table 2 |
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Table 1: Specifications
PN |
Grade |
Particle
Size
(μm) |
Theoretical Plate Number
(per column) |
Exclusion Limit MW
Pullulan |
Max.
pore size (A) |
Max pressure
(MPa) |
CLM3015 |
A2000, Aq GPC/SEC Col |
8 |
> 12,000 |
4,000 |
100 |
5.0 |
CLM3016 |
A2500, Aq GPC/SEC Col |
6 |
> 16,000 |
10,000 |
200 |
5.0 |
CLM3017 |
A3000, Aq GPC/SEC Col |
6 |
> 16,000 |
100,000 |
800 |
5.0 |
CLM3018 |
A4000, Aq GPC/SEC Col |
10 |
> 16,000 |
1,000,000 |
2,000 |
3.0 |
CLM3019 |
A5000, Aq GPC/SEC Col |
13 |
> 12,000 |
(4,000,000) |
7,000 |
3.0 |
CLM3020 |
A6000, Aq GPC/SEC Col |
13 |
> 12,000 |
(20,000,000) |
15,000 |
3.0 |
CLM3021 |
A6000M, Aq GPC/SEC Col |
13 |
> 12,000 |
(20,000,000) |
15,000 |
3.0 |
CLM3022 |
A7000, Aq GPC/SEC Col |
35 |
> 1,500 |
(500,000,000) |
30,000 |
0.5 |
CLM3023 |
AGuard |
10 |
guard column |
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CLM3024 |
A7Guard |
35 |
guard column |
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Table 2: Organic solvent compatibility ranges
Column type |
Methanol |
Acetonitrile |
DMF |
DMSO |
A2000 |
0 % |
0 % |
0 % |
0% |
A2500 - see caution b) |
0 - 100 % |
0 - 75 % |
0 - 100 % |
100% |
A3000 |
0 - 100 % |
0 - 75 % |
0 - 100 % |
0% |
A4000, A5000, A6000, A6000M |
0 - 75 % |
0 - 75 % |
0 - 100 % |
0% |
A7000 |
0 - 30% |
0 - 30% |
0% |
0% |
Note: Protein samples
Urea or 6 M guanidine aqueous solutions, which are commonly used as protein modifiers, can be used as the eluent. An eluent containing surfactant, such as SDS or Brij-35, is recommended for samples such as membrane proteins when their solubility in water is poor.
More information
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Featured presentations and application notes:
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 Application communication: Structural differences in modified starches
The Viscotek triple detection system provides a convenient and rapid way to characterize starches and modified starches. The instrument allows determination of molecular weight and molecular size in a single run using normal conditions and sample concentrations. The IV and size data allow differentiation between molecules of differing structures. The technique is equally applicable to other polysaccharides and all other synthetic or natural polymers such as proteins and DNA. |
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Application Note: Characterization of chitosan from various sources
This note proves that the use of conventional viscometric measurements of chitosan to estimate the molecular weight using Ubbelhode-type viscometer are flawed as the M-H curve exhibits a curvature. However, the Viscotek triple detector was used to study successfully the molecular weight and conformation of chitosan. |
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On-demand presentation: Polysaccharide characterization by triple detection
Triple detection is used with size-exclusion chromatography to characterize polymer distributions. Polysaccharides are natural polymers that can be measured using this technique. Use of triple detection allows molecular weight, size and concentration of polysaccharides to be determined and also giving information about structure. This webinar will describe the technique and provide some application examples. |
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More application notes and online presentations are available to search for FREE in the Malvern Application Library and Presentations Database (Requires Free registration).
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