Gel Permeation Chromatography/Size Exclusion Chromatography (GPC/SEC) systems
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Viscotek Protein SEC Columns

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Viscotek P-columns for protein SEC

Viscotek P-Columns are designed for the separation of biopolymers such as proteins, enzymes and polysaccharides by Size Exclusion Chromatography (SEC). The packing material is a spherical porous silica gel coated with hydrophilic hydroxyl polymer. There are three types of columns for different molecular weight ranges.

Column size:

Analytical columns

300mm L×7.8mm ID

 

Guard column

50mm L×6.0mm ID

Packing material

Porous silica gel coated with chemically-bonded hydroxyl polymer

Connecting screws

Internally-threaded type, No.10 32 UNF

Column material

Stainless steel type SUS-316

Shipping solvent

H2O

Max flow rate

(analysis) 1.5mL/min  (solvent exchange) 0.5mL/min

Temperature Range

10 - 45 ℃

pH range

3.0 - 7.5

Organic solvents

0 – 100% Methanol or Acetonitrile

 

 

Table 1: Specifications

 

PN

Grade

Particle
Size
(μm)

Theoretical Plate Number
(per column)

Exclusion Limit MW

Max.
pore size
(A)

Max.
Pressure
(MPa)

Protein

Pullulan

CLM3025

P2500, Protein SEC Col

5

>21,000

150,000

60,000

400

5.0

CLM3026

P3000, Protein SEC Col

5

> 21,000

700,000

170,000

1,000

5.0

CLM3027

P4000, Protein SEC Col

7

> 16,000

(1,000,000)*

500,000

1,500

5.0

CLM3028

PGuard

7

guard column

* Estimated values

 

More information

 

Featured presentations and application notes:
 
Requires free registration and loginApplication communication: Protein and polymer molecular size by GPC/SEC
The main goal of most Gel Permeation/Size Exclusion Chromatography (GPC/SEC) experiments is to determine the molecular weight distribution (MWD) of the sample or compare molecular weights (MW) of several samples.
  Download the Application Note: Protein and polymer molecular size by GPC/SEC
 
Requires free registration and loginOn-demand presentation: Protein characterization by triple detection
Triple detection is used with size-exclusion chromatography to separate and characterize molecules such as proteins. Using triple detection allows the molecular weight and molecular size of a protein to be calculated independently of each other and retention time. This webinar will describe the technique and provide some example application data.
  Start the on-line presenation: Protein characterization by triple detection

Requires free registration and loginMore application notes and online presentations are available to search for FREE in the Malvern Application Library and Presentations Database (Requires Free registration).