Gel Permeation Chromatography/Size Exclusion Chromatography (GPC/SEC) systems
Viscotek GPC/SEC range

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270max Detectors and Column Oven

Viscotek TDAmax system for temperature controlled Gel Permeation Chromatography
Viscotek TDAmax system for temperature controlled Gel Permeation Chromatography

The Viscotek 270max is a complete, modular, advanced, multi-detector GPC/SEC system suitable for all macromolecular applications. It consists of carefully selected modular units which work seamlessly together for perfect results. The modules are detectors for light scattering, viscometry, RI, and UV, together with a column oven, the GPCmax integrated solvent and sample delivery module and the OmniSEC software.

As a Triple Detector system it employs refractive index, viscometer and light scattering detectors all acting in concert, with each detector providing complimentary data on the macromolecules being analyzed. In its Tetra Detector format, the addition of a UV detector provides a wealth of additional compositional data particularly when the optional Photodiode Array (PDA or DAD) is used to make spectral measurement of all wavelengths simultaneously.

The 270max can be configured with any combination of the 4 detectors:

  • Refractive Index for concentration
  • Viscometer for size and structure
  • Light Scattering for molecular weight
  • UV for chemical composition

In a single GPC/SEC experiment, the 270max provides:

  • Absolute molecular weight without the assumptions, extrapolations or corrections required by alternative techniques.
  • Molecular size expressed as Hydrodynamic Radius (Rh) to less than 1 nm and Radius of Gyration (Rg).
  • Intrinsic viscosity or molecular density.
  • Information on structure, conformation, aggregation and branching.

The 270max detectors and column oven:

 
Light Scattering detector and Viscometer detector within the Dual Detector GPC/SEC system

270 Dual Detector

The Viscotek 270 Dual Detector is the ideal building block for a modular advanced GPC/SEC system. It can be specified as a dual detector (viscometer plus light scattering) or as a single detector, either viscometer or light scattering.
Light Scattering detector and Viscometer detector within the Dual Detector GPC/SEC system
 
Refractive Index Detector for the 270max Gel Permeation Chromatography system

Refractive Index Detector

The accurate 3580 Refractive Index (RI) Detector provides stable and repeatable concentration measurements for GPC/SEC measurements.
Refractive Index Detector for the 270max Gel Permeation Chromatography system
 
UV detector for Gel Permeation Chromatography systems

UV/VIS or Photodiode Array Detector (PDA / DAD)

The Viscotek 270max offers two UV options: a programmable UV-Vis detector or the unique Viscotek PDA, the first one ever integrated in a GPC/SEC system and software.
UV detector for Gel Permeation Chromatography systems
 
Constant temperature GPC/SEC column oven

Column Oven

The VE2585 column oven is specifically designed to achieve the constant column temperature needed for repeatable chromatographic elution profiles.
Constant temperature GPC/SEC column oven

 

More information

 

Featured presentations and application notes:

 
Requires free registration and loginApplication communication: Protein and polymer molecular size by GPC/SEC
The main goal of most Gel Permeation/Size Exclusion Chromatography (GPC/SEC) experiments is to determine the molecular weight distribution (MWD) of the sample or compare molecular weights (MW) of several samples.
  Download the Application Note: Protein and polymer molecular size by GPC/SEC
 
Requires free registration and loginOn-demand presentation: Polysaccharide characterization by triple detection
Triple detection is used with size-exclusion chromatography to characterize polymer distributions. Polysaccharides are natural polymers that can be measured using this technique. Use of triple detection allows molecular weight, size and concentration of polysaccharides to be determined and also giving information about structure. This webinar will describe the technique and provide some application examples.
  Start the on-line presenation: Polysaccharide characterization by triple detection

Requires free registration and loginMore application notes and online presentations are available to search for FREE in the Malvern Application Library and Presentations Database (Requires Free registration).