Particle size analysis, zeta potential measurement, and molecular weight measurement are three of the most important parameters for the colloid and polymer chemist
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Zetasizer Nano Series - Specification
 

"Although we’ve had the Zetasizer Nano only since November last year, it is clearly proving to be the easiest to use and most reliable of all the light scattering systems we’ve had in the lab. Whether sample concentration is low or high, it is never a problem, and is all taken care of with a single button, fully automated measurement. Data are clean and easy to interpret. Whereas light scattering systems we’ve used previously have proved too sensitive to contaminating particles, this is overcome with the Zetasizer Nano."

- Fred Hutchinson Cancer Research Center (SIC)

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Size measurement
Maximum particle size range (diameter):

0.3nm - 10µm**

     
  0.3nm - 5µm††

     
Minimum sample volume:

  12µL

      
  20µL

    
Minimum sample concentration:

   0.1mg/mL Lysozyme
0.1ppm polystyrene latex (60nm)
      
Maximum sample concentration:

   40%w/v*
      
Size measurement in continuous flow for use as SEC chromatography detector :

   Flow rate maximum 1mL/min
     

 

Zeta potential measurement

          
Particle size range (diameter):

   3.8nm - 100µm†

    
Zeta potential range:

   No practical limits exist
    
Electrophoretic mobility range:

   Minimum Zero
No practical upper limit exists

    
Conductivity range:

   0 to 200mS/cm

    
Sample concentration range:

   Up to 40%w/v†

    
Minimum sample volume:

   750µL (Folded capillary cell)
750µL (Dip cell)
150µL (High concentration cell)

    

 

Molecular weight measurement

          
Absolute molecular weight range using Debye plot:

   980Da - 2x107 Da†

      


  9,800Da - 2x107 Da†

      
Molecular weight range estimated from Hydrodynamic diameter:   342Da - 2x107 Da†  
Minimum sample volume:

   12µL

      


  20µL

      
Minimum volume for automated measurement:

  4mL

      

 

Trend measurement

          
Standard:

   Size Vs Time or Temperature

  
  Zeta potential Vs Time or Temperature

    
Using optional MPT-2 autotitrator:

   Size as a function of pH, conductivity or additive concentration

      
  Size as a function of pH, or additive concentration

  
  Zeta potential as a function of pH, conductivity or additive concentration

 

General specifications

          
Temperature range:

   0ºC to 90ºC*
Condensation control:

   Purge facility using dry air.
Patents granted:

   NIBS
DE 19725211
US 6,016,195
JP 2,911,877
EP 0 884 580 designated GB, FR, AU, SW, BE

      
  M3 "High & Low Frequency Electrophoresis"
GB 2,361,772
EP 1 154 266 designated DE, FR
US 09/843339
JP 2001-134510
    
Laser:

   4mW He-Ne, 633nm
Laser attenuator :   Automatic, transmission 100% to 0.0003%
Detector :

   Avalanche photodiode,Q.E > 50% at 633nm
 
Product laser class:

   Class 1 compliant, EN 60825-1:2001 and CDRH
Size :

   320mm, 600mm, 260mm (W,D,H)
Weight :

   21kg
Recommended computer specification:

   Pentium PC 2GHz, 128MByte RAM, 80MByte free hard disk space, 1024 x 768 screen resolution, CD-ROM drive, Windows XP Pro (Service pack 2 or higher) or VISTA Business or Ultimate. One free USB port.

Note. A laptop computer with a USB port can be used with all Zetasizer Nano systems and MPT-2 autotitrator.

 

Options

          
50mW 532nm laser as replacement for standard He-Ne Laser:

   For the measurement of samples not compatible with the standard 633nm He-Ne laser

      
Narrow band filter:

   To improve signal quality for fluorescent samples

Advanced data processing software:

   Advanced system utilities and additional analysis algorithms

Regulatory compliance:

   21 CFR part 11 operating mode software.
Extended temperature:

   Increase of temperature range to 0°C - 120°C

 

Site requirements

         
Power requirements:

   AC 100-240V, 50-60Hz, 100W
Ambient operating Temperature:

   10ºC - 35ºC.
Humidity :

   10 – 90% non-condensing.

Location:

   Normal laboratory conditions, out of direct sunlight.
Dry air for operation at temperatures below the dew point :

   DIN8573-1, oil class 1, water class 3, pressure 200+/-20kPag. Fitting M5 female thread to BS3643.


Note:

* Temperature accuracy, 0.1°C at 25°C, 0.2°C at 0°C and 0.5°C at 90°C, 1°C at 120°C

** Peak mode range (diameter), 0.6nm - 8.9 microns, sample dependent

† Sample dependent

†† Peak mode range (diameter), 0.6nm - 3 microns, sample dependent

 

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