Zetasizer APS
The highest accuracy automated protein size measurement
The Zetasizer APS is a robust, simple to operate, Dynamic Light Scattering automated plate sampler that automates the measurement of samples in industry standard 96- or 384-well plates.
Dual temperature control enables the well plate to be kept at a low temperature to preserve the protein before measurement.
Overview
The Zetasizer APS transfers 20µL sample from each well to a precision quartz flow cell for measurement. The cell environment is optimized for precision temperature control and optical clarity to ensure the most accurate and precise results. This means that any industry standard disposable or reusable plate can be used without concern for transparency or scratches on the plate that would affect the result.
The Zetasizer APS enables you to:
- Investigate your protein over a large number of environmental conditions with minimal user intervention.
- Determine whether a protein is well-behaved in a buffer or whether aggregates are present.
- Assess purity to screen for suitable crystallization buffers.
- Measure aggregation temperature (melting point) to predict or compare stability.
- Uses industry standard 96 or 384 well plates.
- Measures size, size distribution, aggregation temperature.
- Use multiple measurement protocols on a single plate.
- Graphical plate scheduler to simplify set up.
- Color coded results graphic to aid data interpretation.
- Complete plate method stored for subsequent plates.
- Plate sampling technology ensures highest result accuracy.
- 2 - 90°C temperature range for measurement.
- 4 - 40°C temperature range for protein storage.
How it works
The Zetasizer APS (Automated Plate Sampler) uses laser light scattering to determine a number of parameters that are associated with protein conformation and stability.
The system integrates a plate sampling system and measurement unit into a compact box to simplify use.
Dynamic Light Scattering (DLS) determines size by measuring the speed of Brownian movement of particles or molecules in a solvent or buffer. This diffusion speed is converted into a size, polydispersity and size distribution with knowledge of the temperature and buffer viscosity using the Stokes-Einstein relationship.
The size is used to determine oligomeric state, polydispersity provides the purity, and the size distribution is used to detect aggregates. Light scattering is very sensitive to the presence of aggregates, so is an ideal technique to determine the early stages of instability.
Specification
- Measurement type 1
- Particle size and molecular size
- Measurement range:
- 0.3nm – 2.0 microns* (diameter)
- Measurement principle:
- Dynamic Light Scattering
- Minimum sample volume:
- 20µL
- Accuracy:
- Better than +/-2% on NIST traceable latex standards
- Precision/Repeatability
- Better than +/-2% on NIST traceable latex standards
- Sensitivity
- 0.1mg/mL 15kDa protein (Lysozyme)
- Measurement type 2
- Aggregation temperature (melting point)
- Measurement range
- 2ºC - 90°C +/- 0.1.**
- Measurement principle
- Dynamic light scattering
- Minimum sample volume
- 20µL
- Temperature control range
- 2ºC - 90ºC +/- 0.1.**
- Compatible plate types
- 96, 384 well (SBS standard) †
- Light source
- Laser 830nm, max 60mW
- Laser safety
- Class 1
- Power (V, Hz, VA)
- 100VA
- Light source
- Laser 830nm, Max 60mW
- Laser safety
- Class 1
- Dimensions (W, D, H)
- 500mm x 410mm x 420mm (W, D, H)
- Weight
- 20kg
- Operating environment
- Temperature
- 15°C – 40°C
- Humidity
- 35% - 80% non-condensing
- Software
- Recommended computer specification
- Intel i5 processor PC, 4GByte RAM, 500GByte hard disk drive, 1280 x 800 screen resolution (widescreen), or 1280 x 960 (4:3), CD-ROM drive, USB port
- Operating platforms
- Windows XP, Windows 7, compatible with both 32bit and 64bit operating systems
- *
- Maximum range, sample dependent.
- **
- At 25°C
Accessories
Temperature range - 4°C to 40°C
Weight - 12.2kg
Dimensions - W:D:H, 200mm x 385mm x 310mm.
Power - 100V-240V AC, 50/60, 228W
