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Microrheology involves tracking the motion of dispersed tracer particles of known size by Dynamic Light Scattering (DLS) and determining the rheological properties of the sample using the Generalized Stokes-Einstein Relation. DLS Microrheology is a passive microrheology technique, whereby the colloidal probe particles only undergo thermal fluctuations in a system at thermodynamic equilibrium.

DLS Microrheology is applicable for the rheological characterization of low viscosity and weakly-structured complex fluids, such as dilute solutions of polymers and surfactants through to concentrated protein formulations. For these types of materials, Microrheology offers significant advantages:

  • Probes very high frequencies necessary for characterizing inherent short timescale, viscoelastic responses (whereas mechanical techniques are fundamentally limited by inertia).
  • Only requires microliter-scale volumes for rheological characterization of materials where sample volume is limited e.g. protein-based formulations.

Applications of DLS Microrheology include:

  • Rheological characterization of therapeutic proteins and biopolymer solutions.
  • Viscoelastic measurements of protein solutions to assess onset of protein-protein interactions and insoluble aggregate formation
  • Formulation development and screening.
  • High frequency rheology of dilute systems on process-relevant timescales.
  • Monitoring structure development in complex fluids with time or temperature, or structure breakdown on dilution.

Related products

Zetasizer Nano ZSP Sensitivity, simplicity, versatility Image
Measurement
Molecular size, Particle size, Molecular weight, Zeta potential, Protein mobility, Microrheology
Particle size range
0.3nm to 10µm
Temperature range
0°C to 90°C
Dispersion type
Wet
Technology
Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering
More information
Zetasizer Nano ZS Performance, simplicity, versatility. Image
Measurement
Molecular size, Molecular weight, Particle size, Zeta potential
Temperature range
0°C to 90°C
Particle size range
0.3nm to 10µm
Dispersion type
Wet
Technology
Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering
More information
 
 

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Measuring the rheology of polymer solutions Whitepaper (English)

The whitepaper discusses some basic principles of polymer solution rheology and how it can be measured and manipulated to optimize rheological behaviour 


Keywords: Polymer solutions English Whitepaper Rheology and viscosity Rheometry - rotational Polysaccharides Microrheology Rheometry - microfluidic flow 

Optimised Methodology for Dynamic Light Scattering (DLS) Microrheology Poster (English)

This poster presents an optimized methodology for making microrheology measurements on a Zetasizer Nano ZS/ZSP


Keywords: English Poster Dynamic Light Scattering Zetasizer Nano range Microrheology 

Extending the Rheological Characterization of Complex Fluids through DLS Microrheology, Rotational Rheometry and Microfluidics Poster (English)

This poster discusses ways to extend the shear rate and frequency range beyond traditional limits of a rotational rheometer range using creep testing, microrheology and microfluidics


Keywords: English Poster Kinexus range Rheology and viscosity Zetasizer Nano ZSP Microrheology m-VROCi Rheometry - microfluidic flow 

Using Macro- and Microrheology to Characterize the Dynamic Spectrum and Viscoelastic Properties of Macromolecules in Solution Poster (English)

This poster shows why rotational rheometry and microrheology are complementary tools for characterizing the viscoelastic properties of macromolecules in solutions


Keywords: English Poster Kinexus range Rheology and viscosity Rheometry - rotational Zetasizer Nano ZSP Microrheology 

Use of the new MicroCal PEAQ-ITC system for measurement and characterization of a broad range of protein-LMW compound interactions Whitepaper (English)

Measurement and characterization of binding interactions between proteins and low-molecular weight (LMW) ligands are a focus of academic research and drug discovery. Isothermal titration calorimetry (ITC) directly measures heat released or absorbed i...


Keywords: English Whitepaper Microrheology Isothermal Titration Calorimetry Label-free analysis MicroCal PEAQ-ITC range 
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