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Rheology is the study of flow and deformation of materials. Rheological properties, such as viscosity and viscoelasticity, can be measured from bulk sample deformation using a mechanical rheometer, or on a micro-scale using an optical technique such as Microrheology.

Microrheology involves tracking the motion of dispersed tracer particles of known size by Dynamic Light Scattering (DLS) and determining the rheological properties of the sample using the Generalized Stokes-Einstein Relation. DLS Microrheology is a passive microrheology technique, whereby the colloidal probe particles only undergo thermal fluctuations in a system at thermodynamic equilibrium.

DLS Microrheology is applicable for the rheological characterization of low viscosity and weakly-structured complex fluids, such as dilute solutions of polymers and surfactants through to concentrated protein formulations. For these types of materials, Microrheology offers significant advantages:

  • Probes very high frequencies necessary for characterizing inherent short timescale, viscoelastic responses (whereas mechanical techniques are fundamentally limited by inertia).
  • Only requires microliter-scale volumes for rheological characterization of materials where sample volume is limited e.g. protein-based formulations.

Applications of DLS Microrheology include:

  • Rheological characterization of therapeutic proteins and biopolymer solutions.
  • Viscoelastic measurements of protein solutions to assess onset of protein-protein interactions and insoluble aggregate formation
  • Formulation development and screening.
  • High frequency rheology of dilute systems on process-relevant timescales.
  • Monitoring structure development in complex fluids with time or temperature, or structure breakdown on dilution.

Related products

Zetasizer Nano ZSP Sensitivity, simplicity, versatility Image
Measurement
Molecular size, Particle size, Molecular weight, Zeta potential, Protein mobility, Microrheology
Particle size range
0.0003µm to 10µm
Temperature range
90°C
Dispersion type
Wet
Technology
Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering
More information
Zetasizer Nano ZS Performance, simplicity, versatility. Image
Measurement
Molecular size, Molecular weight, Particle size, Zeta potential
Temperature range
90°C
Particle size range
0.0003µm to 10µm
Dispersion type
Wet
Technology
Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering
More information
 
 

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DLS Microrheology on the Zetasizer Nano ZSP/ZS: Use of a zeta potential pre-measurement step to assess tracer particle-sample interactions Application Note (English)

This technical note describes the practical aspects to consider when making a microrheology measurement by dynamic light scattering, and how zeta potential can be used as an indicator of the interaction between tracer particle and sample.


Keywords: English Application Note Zeta potential Electrophoretic Light Scattering Zetasizer Nano ZS Proteins Zetasizer Nano ZSP Microrheology 

Probing low viscosity and weakly-structured complex fluid formulations using DLS microrheology Webinar - Recorded (English)

This webinar is of interest to anyone involved in the rheological testing of complex fluid formulations which are low viscosity and weakly-structured. DLS microrheology is a technique which is particularly applicable to polymers and prote...

Date recorded:
May 27 2014
Language:
English

Keywords: English Rheology and viscosity Dynamic Light Scattering Webinar - Recorded Eastern Time Microrheology 

Light scattering techniques support formulation development Article (English)

Researchers at Novozymes pharma and Malvern Instruments show how light scattering techniques benefit the study of the stability of human albumins. The studies involve the new Zetasizer ZSP and APS, Viscotek SEC systems, and the Morphology G3-ID


Keywords: English Article Morphologi G3 Chemical identification Molecular size Molecular weight Zeta potential Dynamic Light Scattering Electrophoretic Light Scattering Gel Permeation Chromatography Raman spectroscopy Size Exclusion Chromatography Zetasizer APS Zetasizer Nano range Proteins Microrheology Bioformulation development Viscotek systems Viscotek detectors Morphologi G3-ID 

Determination of protein solution viscosity using DLS microrheology Application Note (English)

An application note to demonstrate how Dynamic Light Scattering (DLS) based microrheology can be used to accurately measure the complex viscosity of protein solutions.


Keywords: English Application Note Dynamic Light Scattering Proteins Zetasizer Nano ZSP Microrheology Bioformulation development 

An Introduction to DLS Microrheology Whitepaper (English)

Dynamic Light Scattering microrheology is a passive technique that used tracer particles probe linear viscoelastic properties of low viscosity, weakly structured fluids. The measurements are fast and require only microliter volumes of sample


Keywords: English Whitepaper Rheology and viscosity Dynamic Light Scattering Zetasizer Nano range Microrheology 
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