Skip to left navigation

Skip to page content

Find us on...

The size or hydrodynamic radius RH can be determined in two ways. The first method is by Dynamic Light Scattering (DLS), which is generally used as a batch technique to measure the average size in the whole sample, but can be used as a detector for Size Exclusion Chromatography (GPC/SEC).

A very accurate and precise method of obtaining RH of all components in a sample is by triple detection after separation by SEC.

By the use of online light scattering and viscometer detectors, the MW and intrinsic viscosity of the polymer or protein can be determined and hence the RH calculated at any point on the chromatogram.

The benefit of both these techniques is that there is no practical upper or lower size limit for the materials being investigated.

The measurement of molecular size is a useful parameter for a number of applications:

  • Comparison with molecular weight gives information about structure
  • DLS can use molecular size to give a quick estimate of molecular weight
  • DLS can give a fast screen of protein purity
  • Determination of protein melting point.

Related products

Zetasizer range The world's most widely used system for nanoparticle, colloid and protein size, zeta potential and molecular weight measurements. Image
Measurement
Molecular size, Particle size, Molecular weight, Zeta potential
Temperature range
90°C
Particle size range
0.3nm to 10µm
Dispersion type
Wet
Technology
Dynamic Light Scattering, Static Light Scattering, Size Exclusion Chromatography, Electrophoretic Light Scattering
More information
OMNISEC The world’s most advanced multi-detector GPC/SEC system Image
Measurement
Molecular weight, Molecular size, Molecular structure
Molecular weight range
200Da to 10MDa
Minimum sample mass (polystyrene)
100ng
Minimum sample mass (BSA)
100ng
Technology
Size Exclusion Chromatography, Static Light Scattering, Gel Permeation Chromatography
More information
Viscotek range The leading multi-detector GPC/SEC systems and detectors. Image
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Molecular weight range
1000Da to 10MDa
Minimum sample mass (polystyrene)
1µg
Minimum sample mass (BSA)
1µg
Technology
Dilute Solution Viscometry, Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
More information
Viscosizer TD Automating ultra-low volume Taylor Dispersion Analysis Image
Measurement
Molecular size, Rheology and viscosity
Particle size range
0.0002µm to 0.1µm
Technology
Taylor Dispersion Analysis
More information
 
 

Resource center search

Free access to exclusive content including webinars, presentations, application notes, technical notes, whitepapers and more. Enter your search term in the box and use the drop down boxes to filter the results.

Reset filters
Items shown: 5, 10, 20, ALL
Sort by: Date or Alphabetical
Previous page Showing results 1 - 5 of 124 Next page
Structure-molecular weight relationships of synthetic polymers Application Note (English)

In this application note, the structural distribution of some common polymers are compared using the Mark-Houwink plot. The data are all generated using the advanced OMNISEC multi-detector GPC/SEC system


Keywords: English Application Note Viscotek range Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography OMNISEC 

Assessing the self-association and stability of Insulin under varying formulation conditions using Taylor Dispersion Analysis Application Note (English)

Using Insulin as a case study, Taylor Dispersion measurements are used to monitor self-association and oligomeric state in a variety of buffer conditions, and have the ability to render surfactants and excipients invisible in measurement data.


Keywords: English Application Note Molecular size Proteins Biological drug characterization and development Taylor Dispersion Analysis Viscosizer TD Protein stability 


Understanding Taylor Dispersion Analysis Whitepaper (English)

Taylor Dispersion Analysis is an ultra-low volume, microcapillary flow technique for sizing and stability of biomolecules in complex solutions, and offers advantages for orthogonal and complementary biophysical characterization measurements


Keywords: English Whitepaper Molecular size Taylor Dispersion Analysis Viscosizer TD Protein stability 

Assessing the self-association behavior of Insulin with concentration using Taylor Dispersion Analysis Application Note (English)

TDA with UV-detection is used to follow self-association of protein-based systems, and critically is able resolve the smaller monomeric species since measurements are not adversely affected by the presence of a small amount of oligomer in mixtures.


Keywords: English Application Note Molecular size Proteins Biological drug characterization and development Taylor Dispersion Analysis Viscosizer TD Protein stability 
Previous page Showing results 1 - 5 of 124 Next page

Glossary

Contact sales Register now