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The size or hydrodynamic radius RH can be determined in two ways. The first method is by Dynamic Light Scattering (DLS), which is generally used as a batch technique to measure the average size in the whole sample, but can be used as a detector for Size Exclusion Chromatography (GPC/SEC).

A very accurate and precise method of obtaining RH of all components in a sample is by triple detection after separation by SEC.

By the use of online light scattering and viscometer detectors, the MW and intrinsic viscosity of the polymer or protein can be determined and hence the RH calculated at any point on the chromatogram.

The benefit of both these techniques is that there is no practical upper or lower size limit for the materials being investigated.

The measurement of molecular size is a useful parameter for a number of applications:

  • Comparison with molecular weight gives information about structure
  • DLS can use molecular size to give a quick estimate of molecular weight
  • DLS can give a fast screen of protein purity
  • Determination of protein melting point.

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Structural elucidation of dextrans and other polysaccharides using OMNISEC Multi-Detection GPC Application Note (English)

A series of dextran samples ranging in molecular weight from about 1– 650 kDa are characterized using OMNISEC. Structural comparisons to other polysaccharides, gum arabic and pectin are presented


Keywords: Polymer solutions English Application Note Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers from renewable sources Polysaccharides OMNISEC 

Optimising food additive choice: Exploring the value of recent advances in SEC technology Article (English)

State-of-the-art GPC/SEC systems, such as OMNISEC, offer the sensitivity required to precisely evaluate different grades of polymer-based food additive and to make an optimal choice in formulation


Keywords: English Article Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Food additives Polysaccharides OMNISEC 

The Benefits of Multi-Detector SEC for Protein Analysis Article (English)

Using a light scattering detector as part of a multi-detector Size Exclusion Chromatography system provides the accurate molecular weight (MW) data needed to assess the activity, safety and clinical efficacy of therapeutic proteins.


Keywords: English Article Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Protein aggregation Proteins Biopharmaceutical development Viscotek TDAmax Viscotek TDA 305 

Measuring the 2nd Virial Coeffient and Molecular Density of Proteins to Improve Crystallization Poster (English)

The second virial coefficient of Human Serum Albumin (HSA) was measured using a SEC-MALS 20 detector connected to a Refractive Index concentration detector and an online differential viscometer. Two different buffer conditions, Phosphate buffer pH 7....


Keywords: English Poster Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Proteins Biopharmaceutical development Viscotek SEC-MALS Viscotek TDA 305 

An investigation into the effects of aging on automotive bumper samples by xylene soluble flow injection polymer analysis (XS-FIPA) Poster (English)

This poster describes the use of flow injection polymer analysis (FIPA) as an analytical tool for xylene soluble (XS) determination with quick and easy simultaneous measurements of molecular weight (MW), intrinsic viscosity (IV) and XS%. The techniqu...


Keywords: Polymer solutions English Poster Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Automotive and aerospace Viscotek TDAmax 
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