Skip to left navigation

Skip to page content

Find us on...

The size or hydrodynamic radius RH can be determined in two ways. The first method is by Dynamic Light Scattering (DLS), which is generally used as a batch technique to measure the average size in the whole sample, but can be used as a detector for Size Exclusion Chromatography (GPC/SEC).

A very accurate and precise method of obtaining RH of all components in a sample is by triple detection after separation by SEC.

By the use of online light scattering and viscometer detectors, the MW and intrinsic viscosity of the polymer or protein can be determined and hence the RH calculated at any point on the chromatogram.

The benefit of both these techniques is that there is no practical upper or lower size limit for the materials being investigated.

The measurement of molecular size is a useful parameter for a number of applications:

  • Comparison with molecular weight gives information about structure
  • DLS can use molecular size to give a quick estimate of molecular weight
  • DLS can give a fast screen of protein purity
  • Determination of protein melting point.

Related products

Zetasizer range The worlds most widely used system for nanoparticle, colloid and protein size, zeta potential and molecular weight measurements. Image
Measurement
Molecular size, Particle size, Molecular weight, Zeta potential
Temperature range
90°C
Particle size range
0.3nm to 10µm
Dispersion type
Wet
Technology
Dynamic Light Scattering, Static Light Scattering, Size Exclusion Chromatography, Electrophoretic Light Scattering
More information
OMNISEC The world’s most advanced multi-detector GPC/SEC system Image
Measurement
Molecular weight, Molecular size, Molecular structure
Temperature range
20°C to 65°C
Technology
Size Exclusion Chromatography, Static Light Scattering, Gel Permeation Chromatography
More information
Viscotek range The leading multi-detector GPC/SEC systems and detectors. Image
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Temperature range
25°C to 160°C
Technology
Dilute Solution Viscometry, Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
More information
Viscosizer 200 Automating low volume viscosity and size analysis Image
Measurement
Molecular size, Rheology and viscosity
Particle size range
0.0002µm to 0.1µm
Technology
UV area imaging
More information
 
 

Resource center search

Free access to exclusive content including webinars, presentations, application notes, technical notes, whitepapers and more. Enter your search term in the box and use the drop down boxes to filter the results.

Reset filters
Items shown: 5, 10, 20, ALL
Sort by: Date or Alphabetical
Previous page Showing results 1 - 5 of 150 Next page
OMNISEC for unsurpassed synthetic polymer characterization Webinar - Recorded (English)

Realize the maximum potential of your polymer using unsurpassed characterization of molecular weight and structure using OMNISEC – our latest gel permeation chromatography / size exclusion chromatography system. Dr. Mark Pothecary, Product Manager, ...

Product:
OMNISEC
Date recorded:
February 17 2015
Language:
English

Keywords: Polymer solids and melts Polymer solutions English Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers, plastics and rubbers Polysaccharides Webinar - Recorded Eastern Time OMNISEC 

The use of triple detection GPC to determine absolute molecular and structural information about low molecular weight heparins Poster (English)

In this work three commercially available Low Molecular Weight Heparin samples (Enoxaparin, Tinzaparin and Dalteparin) are measured by triple-detection GPC and any differences identified. In addition, structural comparisons will be made with a commer...


Keywords: English Poster Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Polysaccharides Pharmaceutical development OMNISEC 

Understanding the effect of molecular weight and structure on the performance characteristics of cellulose derivatives using OMNISEC Application Note (English)

A variety of aqueous (water-soluble) cellulose derivatives were characterized using the OMNISEC triple detection GPC/SEC system which revealed subtle, yet distinct differences between them. The differences described in this application note could be ...


Keywords: Polymer solutions English Application Note Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers from renewable sources Pharmaceutical development Excipients and coatings OMNISEC 

Characterization of IgG monomers & their aggregates Application Note (English)

Two antibody samples were characterized using column calibration and multi-detection SEC, and the results compared to show how multi-detection SEC provides more accurate data and a more complete characterization of the protein mixtures under study.


Keywords: English Application Note Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Protein aggregation Proteins Biopharmaceutical development Antibodies and derivatives OMNISEC 

The Mark–Houwink plot – Differentiation of Branching and Composition Application Note (English)

Using OMNISEC, we will show how to separate the effects of a structural difference induced by a compositional change to a polymer (e.g. substitution) and the structural change induced by polymer chain branching.


Keywords: English Application Note Molecular structure Molecular size Molecular weight OMNISEC 
Previous page Showing results 1 - 5 of 150 Next page

Glossary