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The size or hydrodynamic radius RH can be determined in two ways. The first method is by Dynamic Light Scattering (DLS), which is generally used as a batch technique to measure the average size in the whole sample, but can be used as a detector for Size Exclusion Chromatography (GPC/SEC).

A very accurate and precise method of obtaining RH of all components in a sample is by triple detection after separation by SEC.

By the use of online light scattering and viscometer detectors, the MW and intrinsic viscosity of the polymer or protein can be determined and hence the RH calculated at any point on the chromatogram.

The benefit of both these techniques is that there is no practical upper or lower size limit for the materials being investigated.

The measurement of molecular size is a useful parameter for a number of applications:

  • Comparison with molecular weight gives information about structure
  • DLS can use molecular size to give a quick estimate of molecular weight
  • DLS can give a fast screen of protein purity
  • Determination of protein melting point.

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Characterization of hydrodynamic changes upon cation-binding to proteins Application Note (English)

Cations are specifically recognized by numerous proteins. Despite their small size, their specific interactions with highly charged residues allow them to induce significant conformational changes on their binding proteins. The protein conformational...


Keywords: English Application Note Molecular size Molecular weight Zeta potential Electrophoretic Light Scattering Size Exclusion Chromatography Viruses and vaccines Zetasizer Nano ZS Proteins Protein mobility Biological drug characterization and development Viscotek TDAmax Viscotek TDA 305 

PEGylated proteins: Optimizing conjugation reactions using triple detection SEC Application Note (English)

In this application note, interferon α-5 was conjugated with two different PEG molecules in different reactions. The individual protein and PEG molecules, as well as the PEGylated conjugates are characterized by SEC and their composition, mol...


Keywords: English Application Note Molecular size Molecular weight Static Light Scattering Size Exclusion Chromatography Protein aggregation Proteins Biological drug characterization and development Viscotek TDAmax Viscotek SEC-MALS Viscotek UV-PDA Detector (2600) 

The Science of Beauty - Characterizing Hyaluronic Acid - the molecule behind anti-aging creams Webinar - Recorded (English)

Hyaluronic acid (HA) is a natural polysaccharide, that plays an important role in tissues, such as skin, tendons, muscles, and cartilage. As well as medical applications, It finds wide-spread use in cosmetics, particularly skin creams. In...

Date recorded:
June 25 2014
Language:
English

Keywords: English Molecular size Molecular weight Cosmetics Personal care Polymers, plastics and rubbers Webinar - Recorded Eastern Time 

Protein storage and stability analysis using the Zetasizer Nano ZSP Application Note (English)

Spidroins are large, structural proteins that make up the bulk of spider silk fibres. This application note describes the use of a Zetasizer Nano ZSP to characterize their stability at different temperatures.


Keywords: English Application Note Molecular size Dynamic Light Scattering Protein aggregation Proteins Zetasizer Nano ZSP Biological drug characterization and development Bioformulation development 

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