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The size or hydrodynamic radius RH can be determined in two ways. The first method is by Dynamic Light Scattering (DLS), which is generally used as a batch technique to measure the average size in the whole sample, but can be used as a detector for Size Exclusion Chromatography (GPC/SEC).

A very accurate and precise method of obtaining RH of all components in a sample is by triple detection after separation by SEC.

By the use of online light scattering and viscometer detectors, the MW and intrinsic viscosity of the polymer or protein can be determined and hence the RH calculated at any point on the chromatogram.

The benefit of both these techniques is that there is no practical upper or lower size limit for the materials being investigated.

The measurement of molecular size is a useful parameter for a number of applications:

  • Comparison with molecular weight gives information about structure
  • DLS can use molecular size to give a quick estimate of molecular weight
  • DLS can give a fast screen of protein purity
  • Determination of protein melting point.

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Key considerations when purchasing an advanced GPC/SEC system Whitepaper (English)

Investing in a new GPC/SEC system is a big decision with many factors to consider. This white paper lists the key items that you should think about when buying a new system and discusses the reasons for their importance.  Finally, it discusses what M...



What is Multiple Scattering? FAQ

Multiple scattering refers to the phenomenon where photons scattered from the sample are re-scattered from neighboring particles prior to reaching the instrument detector.



What is a Correlation Function (Correlogram)? FAQ

A correlation function shows the correlation coefficients plotted as a function of delay time



Number Fluctuations: What Are They and How Are They Recognised? FAQ

Number fluctuations are defined as variations in the number of particles within the scattering volume during the course of a dynamic light scattering (DLS) measurement.



Optimizing drug delivery polymer conjugate levels using Advanced Multi-Detector GPC Application Note (English)

The drug delivery polymer polyglutamic acid was conjugated with different amounts of Doxorubicin and characterized using advanced multi-detector GPC to measure drug content, molecular weight and structure


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