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The size or hydrodynamic radius RH can be determined in two ways. The first method is by Dynamic Light Scattering (DLS), which is generally used as a batch technique to measure the average size in the whole sample, but can be used as a detector for Size Exclusion Chromatography (GPC/SEC).

A very accurate and precise method of obtaining RH of all components in a sample is by triple detection after separation by SEC.

By the use of online light scattering and viscometer detectors, the MW and intrinsic viscosity of the polymer or protein can be determined and hence the RH calculated at any point on the chromatogram.

The benefit of both these techniques is that there is no practical upper or lower size limit for the materials being investigated.

The measurement of molecular size is a useful parameter for a number of applications:

  • Comparison with molecular weight gives information about structure
  • DLS can use molecular size to give a quick estimate of molecular weight
  • DLS can give a fast screen of protein purity
  • Determination of protein melting point.

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General Instrument Care of the Viscotek TDAmax GPC/SEC system Technical Note (English)

This technical note details information about maintaining Viscotek GPC/SEC systems to maximize reliability, prevent damage, reduce downtime, recognise imminent issues and minimise service visits



From 0 to 60 – Novel polymer applications in the automotive industry Webinar - Recorded (English)

There is a strong global trend in automotive design that features a continuous increase in the use of conventional, performance, and novel polymeric materials. This is a result of weight savings, design flexibility, and ease of fabrication offered by...

Product:
Viscotek HT-GPC,Viscotek systems,Viscotek TDAmax,Viscotek DSV,Viscotek range
Date recorded:
September 3 2015
Language:
English


Principles of Triple Detection GPC/SEC Whitepaper (English)

Triple detection GPC/SEC combines measurements from multiple detectors to offer not only increased amounts of data, but also information, which is available thanks to the combination of different detectors and would not be obtainable using the indivi...



Characterization of IgG monomers & their aggregates Application Note (English)

Two antibody samples were characterized using column calibration and multi-detection SEC, and the results compared to show how multi-detection SEC provides more accurate data and a more complete characterization of the protein mixtures under study.



Differentiation of polymer branching and composition using the Mark–Houwink plot Application Note (English)

Here, we will show how to separate the effects of a structural difference induced by a compositional change to a polymer (e.g. substitution) and the structural change induced by polymer chain branching, using OMNISEC.


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