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The size or hydrodynamic radius RH can be determined in two ways. The first method is by Dynamic Light Scattering (DLS), which is generally used as a batch technique to measure the average size in the whole sample, but can be used as a detector for Size Exclusion Chromatography (GPC/SEC).

A very accurate and precise method of obtaining RH of all components in a sample is by triple detection after separation by SEC.

By the use of online light scattering and viscometer detectors, the MW and intrinsic viscosity of the polymer or protein can be determined and hence the RH calculated at any point on the chromatogram.

The benefit of both these techniques is that there is no practical upper or lower size limit for the materials being investigated.

The measurement of molecular size is a useful parameter for a number of applications:

  • Comparison with molecular weight gives information about structure
  • DLS can use molecular size to give a quick estimate of molecular weight
  • DLS can give a fast screen of protein purity
  • Determination of protein melting point.

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Zetasizer Nano & NanoSight User Meeting - King of Prussia, PA Classroom based training (English)

Learn More – Discover how to get the best out of your instrument with hands-on one-on-one training sessions. Share More – Share your NanoSight and Zetasizer experience with our user community. See More – Meet technology expe...

Product:
Zetasizer Nano range,NanoSight range
Date:
May 22 2014
Location:
Sheraton Valley Forge, 480 North Gulph Road, King of Prussia, Pennsylvania, PA, USA, 19406
Language:
English
Price:
89

Keywords: English Molecular size Particle count Particle size Zeta potential Dynamic Light Scattering USA Classroom based training Eastern Time Zetasizer Nano range Protein aggregation Biological drug characterization and development NanoSight range Nanoparticle Tracking Analysis 

Zetasizer Nano & NanoSight User Meeting - Westborough, MA Classroom based training (English)

Learn More – Discover how to get the best out of your instrument with hands-on one-on-one training sessions. Share More – Share your NanoSight and Zetasizer experience with our user community. See More – Meet technology experts ...

Product:
Zetasizer Nano range,NanoSight range
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Malvern Instruments, Inc., 117 Flanders Rd., Westborough, Massachusetts, MA, USA, 01581
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Price:
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Keywords: English Molecular size Particle count Particle size Zeta potential Dynamic Light Scattering USA Classroom based training Eastern Time Zetasizer Nano range Protein aggregation Biological drug characterization and development NanoSight range Nanoparticle Tracking Analysis 

Number and volume size distributions Application Note (English)

The nature of a particle size distribution analysis by dynamic light scattering is dependent upon the manner in which the particles involved are weighted; whether by number, volume and intensity.  This application note, focusing on protein aggregatio...


Keywords: English Application Note Molecular size Particle size Dynamic Light Scattering Zetasizer Nano range Protein aggregation Proteins Biological drug characterization and development 

Investigating the effects of excipients on the earliest stages of aggregation - Aggregation inhibition by arginine Application Note (English)

Use of static and dynamic light scattering to characterize the stability of lysozyme in the presence and absence of arginine. SLS is used to assess agregation temperature, while DLS is used to measure the various particle sizes present in the two for...


Keywords: English Application Note Molecular size Particle size Zeta potential Dynamic Light Scattering Static Light Scattering Zetasizer Nano range Protein aggregation Proteins Protein mobility Biological drug characterization and development Bio-formulation development 

Monitoring calcium-Induced conformational changes of Calmodulin through size measurements Application Note (English)

Use of the Viscosizer 200 for sizing analysis of Calmodulin samples and assessment of the technique's complementarity to light scattering analysis in the protein characterization workflow.


Keywords: English Application Note Molecular structure Molecular size Particle size Proteins Biological drug characterization and development Bio-formulation development UV area imaging Viscosizer 200 
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