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The size or hydrodynamic radius RH can be determined in two ways. The first method is by Dynamic Light Scattering (DLS), which is generally used as a batch technique to measure the average size in the whole sample, but can be used as a detector for Size Exclusion Chromatography (GPC/SEC).

A very accurate and precise method of obtaining RH of all components in a sample is by triple detection after separation by SEC.

By the use of online light scattering and viscometer detectors, the MW and intrinsic viscosity of the polymer or protein can be determined and hence the RH calculated at any point on the chromatogram.

The benefit of both these techniques is that there is no practical upper or lower size limit for the materials being investigated.

The measurement of molecular size is a useful parameter for a number of applications:

  • Comparison with molecular weight gives information about structure
  • DLS can use molecular size to give a quick estimate of molecular weight
  • DLS can give a fast screen of protein purity
  • Determination of protein melting point.

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Exploring the role of SEC-MALS in protein and polymer characterization Webinar - Recorded (English)

Size exclusion chromatography (SEC) is a chromatographic technique for the characterization of proteins and biopharmaceuticals, as well as natural and synthetic polymers. Traditional systems comprised a single concentration detector (Conventional Cal...

Product:
Viscotek SEC-MALS,Viscotek 270max,Viscotek TDAmax,Viscotek RImax
Date recorded:
October 17 2014
Language:
English

Keywords: Polymer solutions English Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Food additives Rubbers and elastomers Specialty and novel polymers Membrane proteins Polysaccharides Webinar - Recorded GMT Time Protein aggregation Proteins Bioformulation development Antibodies and derivatives Viscotek 270max Viscotek RImax Viscotek TDAmax Viscotek SEC-MALS 

Powerful protein SEC made simple! Webinar - Recorded (English)

This webinar discusses how Malvern’s protein SEC measurement capabilities within the Viscotek range have developed and improved in the last year. We also introduce the new features that are included in the latest release of OmniSEC, including: Bio...

Product:
Viscotek range,Viscotek RImax,Viscotek DSV,Viscotek 270max,Viscotek TDAmax,Viscotek SEC-MALS
Date recorded:
September 30 2014
Language:
English

Keywords: English Viscotek range Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Webinar - Recorded Eastern Time Protein aggregation Proteins Biopharmaceutical development Viscotek 270max Viscotek DSV Viscotek RImax Viscotek TDAmax Viscotek SEC-MALS 

Getting the most out of Dynamic Light Scattering Article (English)

This article explores the benefits of fully automated, high throughput particle size and formulation stability measurements and presents data to show how a new autosampler for the widely used Zetasizer Nano particle characterisation syste...


Keywords: Films and coatings English Article Molecular size Particle size Dynamic Light Scattering Pigments and toners Paints, inks and coatings Colloids Zetasizer Nano S Zetasizer Nano S90 Zetasizer Nano ZS Zetasizer Nano ZS90 Zetasizer Nano ZSP 

Characterization of Whey Protein Isolate (WPI) using multi-detector size-exclusion chromatography Application Note (English)

In this application note, a sample of Whey Protein Isolate (WPI) was characterized using the Viscotek TDAmax. The 4 main components in this mixture were separated and individually characterized to determine molecular weight and size. ...


Keywords: English Application Note Molecular size Molecular weight Size Exclusion Chromatography Dairy products Food additives Protein aggregation Proteins Viscotek TDAmax Viscotek TDA 305 

Characterization of Protein-Protein Interactions using triple detection size exclusion chromatography (SEC-TD) Application Note (English)

In this application note, triple detection size exclusion chromatography (SEC-TD) is used to understand the ability of proteins to form ordered, structured complexes. The controlling mechanism behind these protein complexes is fundamental to ...


Keywords: English Application Note Molecular size Molecular weight Static Light Scattering Size Exclusion Chromatography Viruses and vaccines Proteins Biological drug characterization and development Viscotek TDAmax Viscotek TDA 305 
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