Skip to left navigation

Skip to page content

Find us on...

Measurements of protein electrophoretic mobility (zeta potential) are becoming more widespread in protein and biopharmaceutical research. It is now becoming recognized that as well as being associated with the charge on the molecule, electrophoretic mobility also relates to the stability and the behavior of the protein in solution.

The charge on a protein is one of the fundamental parameters that affects aspects of protein behavior such as:

  • Activity.
  • Aggregation.
  • Interaction with membranes and other surfaces.
  • Ligand binding affinity.
  • Filtration.
  • Long term storage.
  • Crystallization.
  • Processing.

Measurement of the charge can provide valuable information that assists with formulation development to control these interactions to predict stability and improve shelf life.

The Zetasizer Nano ZSP has the sensitivity, and associated protocols and software to reliably and accurately measure protein mobility in order to study and understand protein formulation behavior.

Related products

Zetasizer Nano ZSP Sensitivity, simplicity, versatility Image
Measurement
Molecular size, Particle size, Molecular weight, Zeta potential, Protein mobility, Microrheology
Particle size range
0.3nm to 10µm
Temperature range
0°C to 90°C
Dispersion type
Wet
Technology
Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering
More information
Zetasizer Nano ZS Performance, simplicity, versatility. Image
Measurement
Molecular size, Molecular weight, Particle size, Zeta potential
Temperature range
0°C to 90°C
Particle size range
0.3nm to 10µm
Dispersion type
Wet
Technology
Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering
More information
 
 

Resource center search

Free access to exclusive content including webinars, presentations, application notes, technical notes, whitepapers and more. Enter your search term in the box and use the drop down boxes to filter the results.

Reset filters
Items shown: 5, 10, 20, ALL
Sort by: Date or Alphabetical
Previous page Showing results 1 - 5 of 12 Next page
Characterization of hydrodynamic changes upon cation-binding to proteins Application Note (English)

Cations are specifically recognized by numerous proteins. Despite their small size, their specific interactions with highly charged residues allow them to induce significant conformational changes on their binding proteins. The protein conformational...


Keywords: English Application Note Zeta potential Electrophoretic Light Scattering Size Exclusion Chromatography Zetasizer Nano range Proteins Protein mobility Biological drug characterization and development 

Pushing the limits of Light Scattering Detection for Biotherapeutic Characterization Webinar - Recorded (English)

This seminar discusses the total characterization of a number of model biomolecules using Dynamic Light Scattering, Electrophoretic Light Scattering and Multi-Angle Light Scattering combined with SEC. Data available from these techniques include size...

Product:
Zetasizer Nano ZSP,Viscotek SEC-MALS,Zetasizer µV
Date recorded:
April 24 2014
Language:
English

Keywords: English Molecular size Molecular weight Particle size Dynamic Light Scattering Electrophoretic Light Scattering Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Webinar - Recorded GMT Time Zetasizer µV Protein aggregation Proteins Zetasizer Nano ZSP Protein mobility Biological drug characterization and development Viscotek SEC-MALS 

Studying the influence of metal ions on the structural stability of carbonic anhydrase with the Zetasizer Nano ZSP: An orthogonal approach Application Note (English)

Use of the Zetasizer Nano ZSP to assess the structural stability of carbonic anhydrase in the presence and absence of zinc ions by measuring hydrodynamic radius and electrophoretic mobility.


Keywords: English Application Note Molecular size Dynamic Light Scattering Electrophoretic Light Scattering Protein aggregation Proteins Zetasizer Nano ZSP Protein mobility Biological drug characterization and development 

Determining protein isoelectric point: A method comparison Application Note (English)

This application note compares the isoelectric point determined using the diffusion barrier technique against that obtained from other charge-measuring techniques. The data shown here shows very good agreement.


Keywords: English Application Note Zeta potential Electrophoretic Light Scattering Proteins Zetasizer Nano ZSP Protein mobility 

The Zeta Potential and New Protein Studies Webinar - Recorded (English)

Measurements of the zeta potential of proteins were challenging due to their poor scattering and sensitivity to the measurement conditions. The Zetasizer Nano ZSP, and a novel measurement technique has made dramatic improvements in results

Product:
Zetasizer Nano ZSP
Date recorded:
April 25 2013
Language:
English

Keywords: English Molecular size Zeta potential Electrophoretic Light Scattering Webinar - Recorded Greenwich Time Zetasizer Nano ZSP Protein mobility 
Previous page Showing results 1 - 5 of 12 Next page

Glossary

Contact sales Register now