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Measurements of protein electrophoretic mobility (zeta potential) are becoming more widespread in protein and biopharmaceutical research. It is now becoming recognized that as well as being associated with the charge on the molecule, electrophoretic mobility also relates to the stability and the behavior of the protein in solution.

The charge on a protein is one of the fundamental parameters that affects aspects of protein behavior such as:

  • Activity.
  • Aggregation.
  • Interaction with membranes and other surfaces.
  • Ligand binding affinity.
  • Filtration.
  • Long term storage.
  • Crystallization.
  • Processing.

Measurement of the charge can provide valuable information that assists with formulation development to control these interactions to predict stability and improve shelf life.

The Zetasizer Nano ZSP has the sensitivity, and associated protocols and software to reliably and accurately measure protein mobility in order to study and understand protein formulation behavior.

Related products

Zetasizer Nano ZSP Sensitivity, simplicity, versatility Image
Measurement
Molecular size, Particle size, Molecular weight, Zeta potential, Protein mobility, Microrheology
Particle size range
0.3nm to 10µm
Temperature range
0°C to 90°C
Dispersion type
Wet
Technology
Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering
More information
Zetasizer Nano ZS Performance, simplicity, versatility. Image
Measurement
Molecular size, Molecular weight, Particle size, Zeta potential
Temperature range
0°C to 90°C
Particle size range
0.3nm to 10µm
Dispersion type
Wet
Technology
Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering
More information
 
 

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Measuring Zeta Potential - Laser Doppler Electrophoresis Explained Technical Note (English)

This technical note describes the technique used to measure zeta potential and discusses the technology used in the market leading range of Zetasizer instruments, the Nano series.



Experimental progress in high conductivity electrophoresis using the Diffusion Barrier Technique Poster (English)

In this work we demonstrate latex measurements up to 4M KCL using the Diffusion Barrier Technique, thereby demonstrating sample-limited, rather than instrument-limited characterization of high conductivity samples.


Keywords: Nanoparticles English Poster Particle size Zeta potential Dynamic Light Scattering Electrophoretic Light Scattering Colloids Zetasizer Nano ZS Proteins Zetasizer Nano ZSP Protein mobility 

Unsurpassed protein SEC using the latest innovation in advanced detection, OMNISEC Webinar - Recorded (English)

During this webinar, we introduces the latest innovations in SEC technology, and discuss how the new OMNISEC system will assist your protein characterization challenges. Focusing on applications, the webinar compares the capabilities of the system wi...

Product:
OMNISEC
Date recorded:
April 22 2015
Language:
English

Keywords: English Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Webinar - Recorded Eastern Time Protein aggregation Proteins Protein mobility OMNISEC Protein stability 

Challenges in the measurement of protein zeta potential Poster (English)

Research shows that aggregation of a protein sample that occurs during electrophoretic measurements is more likely to take place at the electrodes. A new measurement method, the Diffusion Barrier Technique, described in this poster protects the prote...


Keywords: English Poster Particle size Dynamic Light Scattering Electrophoretic Light Scattering Zetasizer Nano ZS Protein aggregation Proteins Zetasizer Nano ZSP Protein mobility Biopharmaceutical development 

Characterization of hydrodynamic changes upon cation-binding to proteins Application Note (English)

Cations are specifically recognized by numerous proteins. Despite their small size, their specific interactions with highly charged residues allow them to induce significant conformational changes on their binding proteins. The protein conformational...


Keywords: English Application Note Zeta potential Electrophoretic Light Scattering Size Exclusion Chromatography Zetasizer Nano range Proteins Protein mobility Biological drug characterization and development 
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