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Measurements of protein electrophoretic mobility (zeta potential) are becoming more widespread in protein and biopharmaceutical research. It is now becoming recognized that as well as being associated with the charge on the molecule, electrophoretic mobility also relates to the stability and the behavior of the protein in solution.

The charge on a protein is one of the fundamental parameters that affects aspects of protein behavior such as:

  • Activity.
  • Aggregation.
  • Interaction with membranes and other surfaces.
  • Ligand binding affinity.
  • Filtration.
  • Long term storage.
  • Crystallization.
  • Processing.

Measurement of the charge can provide valuable information that assists with formulation development to control these interactions to predict stability and improve shelf life.

The Zetasizer Nano ZSP has the sensitivity, and associated protocols and software to reliably and accurately measure protein mobility in order to study and understand protein formulation behavior.

Related products

Zetasizer Nano ZSP Sensitivity, simplicity, versatility Image
Measurement
Molecular size, Particle size, Molecular weight, Zeta potential, Protein mobility, Microrheology
Particle size range
0.3nm to 10µm
Temperature range
90°C
Dispersion type
Wet
Technology
Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering
More information
Zetasizer Nano ZS Performance, simplicity, versatility. Image
Measurement
Molecular size, Molecular weight, Particle size, Zeta potential
Temperature range
90°C
Particle size range
0.3nm to 10µm
Dispersion type
Wet
Technology
Dynamic Light Scattering, Electrophoretic Light Scattering, Static Light Scattering
More information
 
 

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Characterization of hydrodynamic changes upon cation-binding to proteins Application Note (English)

Cations are specifically recognized by numerous proteins. Despite their small size, their specific interactions with highly charged residues allow them to induce significant conformational changes on their binding proteins. The protein conformational...


Keywords: English Application Note Molecular size Molecular weight Zeta potential Electrophoretic Light Scattering Size Exclusion Chromatography Viruses and vaccines Zetasizer Nano ZS Proteins Protein mobility Biological drug characterization and development Viscotek TDAmax Viscotek TDA 305 

Pushing the limits of Light Scattering Detection for Biotherapeutic Characterization Webinar - Recorded (English)

This seminar discusses the total characterization of a number of model biomolecules using Dynamic Light Scattering, Electrophoretic Light Scattering and Multi-Angle Light Scattering combined with SEC. Data available from these techniques include size...

Product:
Zetasizer Nano ZSP,Viscotek TDAmax,Viscotek SEC-MALS,Zetasizer µV
Date recorded:
April 24 2014
Language:
English

Keywords: English Molecular size Molecular weight Particle size Dynamic Light Scattering Electrophoretic Light Scattering Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Webinar - Recorded GMT Time Zetasizer µV Protein aggregation Proteins Zetasizer Nano ZSP Protein mobility Biological drug characterization and development Bioformulation development Antibodies and derivatives Viscotek TDAmax Viscotek SEC-MALS 

Investigating the effects of excipients on the earliest stages of aggregation - Aggregation inhibition by arginine Application Note (English)

Use of static and dynamic light scattering to characterize the stability of lysozyme in the presence and absence of arginine. SLS is used to assess agregation temperature, while DLS is used to measure the various particle sizes present in the two for...


Keywords: English Application Note Molecular size Particle size Zeta potential Dynamic Light Scattering Static Light Scattering Protein aggregation Proteins Zetasizer Nano ZSP Protein mobility Biological drug characterization and development Bioformulation development 


Determining protein isoelectric point: A method comparison Application Note (English)

This application note compares the isoelectric point determined using the diffusion barrier technique against that obtained from other charge-measuring techniques. The data shown here shows very good agreement.


Keywords: English Application Note Zeta potential Electrophoretic Light Scattering Proteins Zetasizer Nano ZSP Protein mobility Biological drug characterization and development 
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