The MicroCal VP-ITC delivers affinity data that provide insight into biomolecular interactions and can be used to investigate any such interaction. Isothermal Titration Calorimetry is label free, and in solution, and is fast, convenient and offers a free choice of buffers and no limitations with respect to molecular weight.

The MicroCal VP-ITC is complete system that requires no additional accessories, reagents or consumables. Once the samples are loaded it operates unattended, freeing the user for other tasks.  

Isothermal Titration Calorimetry (ITC) measures the binding affinity and thermodynamics between any two biomolecules. When substances bind, heat is either released or absorbed. Measurement of this heat change in an ITC experiment enables accurate determination of binding constants (KD), interaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), providing a complete thermodynamic profile of the molecular interaction in a single experiment.

MicroCal VP-ITC can also be used to determine heat capacity changes upon binding by performing experiments over a range of temperatures.

  • Directly measures millimolar to nanomolar affinities (KS) (10-­2 to 10-9 M).
  • Measures nanomolar to picomolar disassociation constants using competitive binding techniques (109 to 1012 M).
  • Nonreactive Hastelloy cells for excellent chemical resistance and applicability with biological samples.
  • Fixed-in-place cells for reproducible, ultrasensitive performance with low maintenance.
  • Precision liquid delivery system for accurate and reproducible injections.
  • Coin shaped cell optimizes sample mixing.
  • Three user-selectable response times for application versatility.
  • User-selectable mixing speeds to match sample conditions.
  • Peltier controlled for rapid temperature equilibration.
  • Includes ThermoVac™ sample preparation and cleaning device.
  • Controlled by VPViewer™ software and data analysis performed with Origin™.
  • True in-solution technique: No labeling or immobilization required. No buffer restrictions. Easily handles turbid solutions.