Skip to left navigation

Skip to page content

Find us on...

Malvern’s OMNISEC is a Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) system for the measurement of absolute molecular weight, molecular size, intrinsic viscosity, branching and other parameters. It includes OMNISEC RESOLVE, the integrated GPC/SEC module, OMNISEC REVEAL, the integrated multi-detector module and OMNISEC software for the characterization of synthetic and natural polymers, and proteins.

Overview

OMNISEC is a powerful tool for taking control of the performance, quality and value of your product, whether that’s a synthetic polymer in industry, a natural polymer or polysaccharide used in food or pharmaceutical products, or a protein or antibody used in biopharmaceuticals.

As a research tool, it accelerates research into these molecules by providing absolute information for a wide range of applications making it an ideal tool for universities. It reduces service expense and downtime and increases productivity with easy-to-use intuitive software.

With OMNISEC you can…Of…
Control strength, toughness, durability, brittleness, processability and branchingPolystyrene, nylon, PET, PVC, polyester, nitrocellulose, resins, epoxys, polyurethanes, PVA
Measure degradation and drug delivery ratesPLA, PLGA, PEG, polycaprolocatone
Grade natural polymers and polysaccharidesAlginates, xanthans, Gum Arabic, starch derivatives (e.g. maltodextrin), cellulose derivatives (e.g. HEC), carageenan, gelatin, guar gum, chitosan, hyaluronic acid
Control formulation viscosityAlkyds, acrylics, polyurethanes, polyesters, resins, epoxys, PVA,
Control food mouth-feel and gellingPectins, alginates, xanthans, Gum Arabic, guar gum, starch derivatives (e.g. maltodextrin), carageenans
Assess and predict activity by measuring molecular weightProteins, antibodies, mAbs
Predict and understand immunogenicity and efficacyAggregates of proteins, biopharmaceuticals, biologics and mAbs
Control half-life, cell permeability and understand crystallization of protein conjugatesPEGylated proteins, membrane proteins, antibody-drug-conjugates

How it works

In size-exclusion chromatography, molecules are separated according to their hydrodynamic radius as they enter and exit the pores of a porous gel packing matrix in the column. Like other HPLC-based techniques, this involves the use of a pump, column and at least one detector to measure the sample as it elutes. An OMNISEC GPC/SEC system includes the following components:

01_Degasser

Degasser

  • Rapid mobile-phase exchange thanks to low volume degasser
02_Pump

Pump

  • Rapid mobile-phase exchange thanks to self-priming pump
  • Reduced service requirement thanks to novel continuous back seal flushing mechanism
03_Autosampler

Autosampler

  • Protect samples from aggregation with autosampler temperature control
  • Use your preferred sample format; vials or 96-well microtiter plates
  • Reduce sample use with zero-waste mode
04_ColumnOven

Column Oven

  • Temperature control of up to 6 analytical columns
05_RI

Refractive Index

  • Measure the concentration of any solute with excellent sensitivity
06_UVvisPDA

UV/Vis PDA

  • Measure absorbance at any wavelength from 190-900 nm
  • Assess changes in absorbance spectra
  • Characterize copolymers
07_LightScattering

Light Scattering

  • Measure absolute molecular weight in a wider range of applications
  • Measure as little as 100 ng of material
  • Measure molecular weights down to 200 Da
08_Viscometer

Viscometer

  • Better intrinsic viscosity measurements thanks to automated balancing
  • Reduced service cost and time thanks to user-exchangeable capillaries

Specification

OMNISEC RESOLVE

Pump flow rate:
0.005 - 10 mL/min
Flow rate accuracy:
±1%
Pressure range:
0 - 5000 PSI (34.5 MPa)
Pulsation:
0.15% @ 1 mL/min in water
Precision:
0.25% RSD
Dimensions (W, D, H):
420, 640, 890 mm
Weight:
62 kg
Software:
OMNISEC software v10 (or later)
Data acquisition rate:
100 Hz
Patents:
US 14/599,033 "Continuous back seal wash"

AUTOSAMPLER

Capacity:
Up to 192
Sample container types:
HPLC vials or 96-well microtiter plates
Injection volume:
1 - 300 µL
Injection volume accuracy:
>99.5%
Injection volume precision:

<0.3% RSD in full loop mode

<0.5% RSD in partial loop mode

<1% RSD in µL pickup mode

Injection overhead volume:
0 µL in µL pickup mode
Syringe volume:
250 µL standard
Temperature control range:
4 - 60 °C

COLUMN OVEN

Column capacity:
6 x analytical (1 x Tricorn 10/300 GL)
Temperature control range:
20 - 65 °C

OMNISEC REVEAL

Dimensions (W, D, H):
420, 640, 600 mm
Weight:
40 kg
Detector temperature range:
20 - 65 °C
Software:
OMNISEC software v10 (or later)
Data acquisition rate:
100 Hz
Patents:

US20140060162A1 & EP2619543Ba "Modular capillary bridge viscometer"

US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"

Detector 1

Detector:
Light scattering
Measurement principle:
RALS 90° angle, LALS 7° angle
Light source:
50 mW, 640 nm laser
Cell volume:
18 µL
Dynamic range:
2500 mV
Baseline noise:
<0.1 mV
Baseline drift:
<0.2 mV/hr

Detector 2

Detector:
Differential refractive index
Measurement principle:
Deflection
Cell volume:
12 µL
Dynamic range:
±2.5 x 10-4 RIU
Baseline noise:
<10-7 RIU
Baseline drift:
<3 x 10-7 RIU/hr

Detector 3

Detector:
Viscometer
Measurement principle:
4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries
Transducers:
Firmware-based transducer overpressure protection
Detector volume:
17 µL/capillary
Differential pressure baseline noise:
0.3 Pa
Inlet pressure dynamic range:
100 kPa
Inlet pressure baseline noise:
0.01 kPa
Baseline drift:
<0.2 kPa/hr

Detector 4

Detector:
Diode-array-based UV/Vis spectrometer
Wavelength:
190 - 900 nm
Number of wavelengths:
1024
Wavelength accuracy:
<1 nm
Wavelength resolution:
0.6 nm
Cell volume:
7.5 µL
Path length:
10 mm
Baseline noise:
2 x 10-5 AU
Baseline drift:
5 x 10-4 AU/hr

Measurement type 1

Measurement type:
Absolute molecular weight
Measurement range:
200 - >107 g/mol
Minimum quantifiable mass:
100 ng of 100kDa molecular weight polystyrene in THF
Measurement principle:
Light scattering (LALS or RALS)

Measurement type 2

Measurement type:
Intrinsic viscosity
Minimum quantifiable mass:
1 µg of 100kDa molecular weight polystyrene in THF
Measurement principle:
4-capillary Wheatstone bridge

Measurement type 3

Measurement type:
Concentration
Minimum quantifiable mass:
100 ng of 100kDa molecular weight polystyrene in THF
Measurement principle:
Differential refractive index detection

Operating environment

Temperature:
15 - 30 °C

Software

Minimum computer specification:
Windows 7 Professional 64 bit, 4th Gen Intel(R) Core I7 Processor (Quad Core HT, 3.4 GHz Turbo, w/ HD Graphics), 8 GB 1600 MHz DDR3 memory, 500 GB 3.5 inch SATA (7200 RPM) HDD, Full-HD monitor

Accessories

OMNISEC system

OMNISEC RESOLVE Combined pump, degasser, autosampler and column oven, for high quality GPC/SEC and accurate molecular weight measurement. Image OMNISEC RESOLVE combines with OMNISEC REVEAL to form a complete OMNISEC GPC/SEC solution for the measurement of absolute molecular weight, intrinsic viscosity and other parameters. It combines an integrated pump, degasser, autosampler and column oven in a single advanced separations unit. More information
OMNISEC REVEAL The ultimate in integrated advanced detection for GPC/SEC Image Molecular weight, intrinsic viscosity and numerous other parameters can be measured with OMNISEC REVEAL, the world’s most advanced integrated GPC/SEC multi-detector available. It can be added to any existing conventional GPC/SEC system or used in conjunction with OMNISEC RESOLVE to form a complete GPC/SEC solution, to offer comprehensive molecular characterization of synthetic and natural polymers, as well as proteins. More information
OMNISEC software Powerful, agile, easy to use GPC/SEC software for OMNISEC instrument control, data acquisition, analysis and reporting Image OMNISEC software is an integrated solution for the advanced analysis of proteins and polymers by GPC/SEC, and is specifically designed for control of OMNISEC RESOLVE and OMNISEC REVEAL. Simplified workflows improve productivity and help you to maximize the information and efficiency of your experiments. Simply load your samples and the software controls all the sequencing, enabling truly automatic unattended operation. More information
GPC and SEC Columns GPC/SEC columns for all aqueous and organic applications Image A wide range of columns for analytical GPC/SEC of synthetic and natural polymers and proteins. More information
GPC and SEC Standards A range of molecular weight standards for different applications of GPC/SEC Image Malvern offers a wide range of molecular weight standards for GPC/SEC for conventional and universal calibration as well as light scattering. More information

Support

When you purchase a Malvern product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Some customers just need to ask an occasional question or to download a software upgrade, whilst others require a comprehensive support contract with priority call out and fixed cost of ownership. Whatever your situation Malvern will provide the right support for you.

Help Desk

  • Telephone and email support.
  • Operators who take responsibility for your call and ensure your query is answered. 

Training

  • On Site training courses
  • Classroom training
  • E-learning

Resource center

  • Application notes
  • Technical notes
  • On demand presentations
  • Live webinars

Software downloads

  • A simple mechanism for receiving updates and new features

Platinum and Gold Service Contracts

  • Protection from the cost of downtime
  • No time lost in obtaining help when you need it
  • Fixed cost of ownership

Bronze Service Agreements

  • Protect the value of your investment
  • Reduce the risk of downtime
  • Protect the validity of your data
 
 

Resource center search

Free access to exclusive content including webinars, presentations, application notes, technical notes, whitepapers and more. Enter your search term in the box and use the drop down boxes to filter the results.

Reset filters
Items shown: 5, 10, 20, ALL
Sort by: Date or Alphabetical
Previous page Showing results 1 - 5 of 27 Next page
Structure-molecular weight relationships of synthetic polymers Application Note (English)

In this application note, the structural distribution of some common polymers are compared using the Mark-Houwink plot. The data are all generated using the advanced OMNISEC multi-detector GPC/SEC system


Keywords: English Application Note Viscotek range Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography OMNISEC 

Improve your understanding of the effect of polysaccharide molecular weight & structure on final product performance Webinar - Live (English)

From simple food additives to complex material used in high-tech eye surgery and targeted drug delivery systems, polysaccharides have earned unparalleled attention when it comes to macromolecular characterization. With more emphasis on product perfor...

Product:
OMNISEC
Date:
July 8 2015
Language:
English

Keywords: English Molecular structure Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Webinar - Live Eastern Time OMNISEC 

Ten reasons protein characterization scientists should upgrade from single to multi-detector SEC Whitepaper (English)

The top 10 reasons to consider an advanced multi-detector SEC system, which incorporates light scattering detectors for the measurement of absolute molecular weight and molecular weight distribution with excellent accuracy.


Keywords: English Whitepaper Viscotek range Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Membrane proteins Viruses and vaccines Protein aggregation Proteins Biopharmaceutical development OMNISEC 

Malvern polyacrylonitrile characterization package Webinar - Recorded (English)

Gel Permeation Chromatography (GPC) also known as Size Exclusion Chromatography (SEC), is a very popular analytical tool for characterizing natural and synthetic polymers. This seminar will show excellent results possible with our Triple Detection G...

Product:
OMNISEC
Date recorded:
June 24 2015
Language:
English

Keywords: English Gel Permeation Chromatography Size Exclusion Chromatography Webinar - Recorded Eastern Time OMNISEC 

Ten reasons polymer characterization scientists should upgrade from single to multi-detector GPC Whitepaper (English)

The top ten reasons to consider an advanced multi-detector GPC system, which incorporates light scattering detectors for the measurement of absolute molecular weight and molecular weight distribution with excellent accuracy.


Keywords: Polymer solutions English Whitepaper Viscotek range Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers, plastics and rubbers Food additives Polysaccharides OMNISEC 
Previous page Showing results 1 - 5 of 27 Next page
Request a demo Request a quote Contact sales Register now
 

Upcoming webinar

Improve your understanding of the effect of polysaccharide molecular weight & structure on final product performance
(July 8 2015)

More information
Download brochure