Skip to left navigation

Skip to page content

Find us on...

Malvern’s OMNISEC is a Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) system for the measurement of absolute molecular weight, molecular size, intrinsic viscosity, branching and other parameters. It includes OMNISEC RESOLVE, the integrated GPC/SEC module, OMNISEC REVEAL, the integrated multi-detector module and OMNISEC software for the characterization of synthetic and natural polymers, and proteins.

Overview

Over 30 years of GPC/SEC experience has enabled the development of the most sensitive and accurate multi-detector system available. OMNISEC RESOLVE combines an integrated degasser, isocratic pump, autosampler and column oven to give reduced baseline noise, and maximum measurement sensitivity and accuracy. OMNISEC REVEAL’s optimized detectors include light scattering, refractive index (RI), UV/Vis photodiode array and viscometer enabling the measurement of samples with very low concentrations or refractive index increments.

With OMNISEC you can:

  • Understand and control product performance of polymers, polysaccharides and proteins by measuring absolute molecular weight, intrinsic viscosity, hydrodynamic radius, Mark-Houwink parameters, radius of gyration, branching, and co-polymer composition
  • Measure molecular weight as low as 200 Da or injection masses as low as 100 ng for characterization of samples which were previously unmeasurable using advanced detection. 
  • Reduce the cost of measurement of precious samples with low injection volumes; waste-free injections and by making injections from 96-well microtiter plates
  • Improve measurement accuracy and repeatability by reducing sample degradation in the temperature-controlled autosampler
  • Improve separations with the column oven and temperature-controlled integrated detector compartment that minimizes inter-detector tubing
  • Undertake a wide range of applications in different solvents thanks to Teflon and 316 stainless steel construction throughout
  • Reduce instrument downtime and service costs with the low-volume degasser and continuous pump seal back washing
  • Boost lab productivity with a novel self-balancing viscometer design with user-exchangeable capillaries.

How it works

GPC/SEC is a two-step analytical process that involves separating a sample on the basis of its size and then analyzing the resulting fractions. The technique delivers molecular weight, molecular size and structural information, all of which define the performance of many industrially important materials.  Coupling the efficient sample separation of OMNISEC RESOLVE with the fully optimized, multi-detector, information-rich analysis delivered by OMNISEC REVEAL, Malvern has produced a market-leading solution for researchers and formulators developing novel natural and synthetic polymers, pharmaceutical and biopharmaceutical products and foods.

The RI and UV detectors give information about the concentration of the components in the sample. The light scattering detectors respond to the intensity of light scattered by the sample which is related to molecular weight and also allows Rg of large molecules to be calculated. The viscometer measures the changing solution viscosity to calculate the intrinsic viscosity of the sample which is a measure of structure. By combining the molecular weight and intrinsic viscosity data, the hydrodynamic diameter of the sample can also be calculated along with structural information via the Mark-Houwink plot.

Specification

OMNISEC RESOLVE

Pump flow rate:
0.005 - 10 mL/min
Flow rate accuracy:
±1%
Pressure range:
0 - 5000 PSI (34.5 MPa)
Pulsation:
0.15% @ 1 mL/min in water
Precision:
0.25% RSD
Dimensions (W, D, H):
420, 640, 890 mm
Weight:
62 kg
Software:
OMNISEC software v10 (or later)
Data acquisition rate:
100 Hz
Patents:
US 14/599,033 "Continuous back seal wash"

AUTOSAMPLER

Capacity:
Up to 192
Sample container types:
HPLC vials or 96-well microtiter plates
Injection volume:
1 - 300 µL
Injection volume accuracy:
>99.5%
Injection volume precision:

<0.3% RSD in full loop mode

<0.5% RSD in partial loop mode

<1% RSD in µL pickup mode

Injection overhead volume:
0 µL in µL pickup mode
Syringe volume:
250 µL standard
Temperature control range:
4 - 60 °C

COLUMN OVEN

Column capacity:
6 x analytical (1 x Tricorn 10/300 GL)
Temperature control range:
20 - 65 °C

OMNISEC REVEAL

Dimensions (W, D, H):
420, 640, 600 mm
Weight:
40 kg
Detector temperature range:
20 - 65 °C
Software:
OMNISEC software v10 (or later)
Data acquisition rate:
100 Hz
Patents:

US20140060162A1 & EP2619543Ba "Modular capillary bridge viscometer"

US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"

Detector 1

Detector:
Light scattering
Measurement principle:
RALS 90° angle, LALS 7° angle
Light source:
50 mW, 640 nm laser
Cell volume:
18 µL
Dynamic range:
2500 mV
Baseline noise:
<0.1 mV
Baseline drift:
<0.2 mV/hr

Detector 2

Detector:
Differential refractive index
Measurement principle:
Deflection
Cell volume:
12 µL
Dynamic range:
±2.5 x 10-4 RIU
Baseline noise:
<10-7 RIU
Baseline drift:
<3 x 10-7 RIU/hr

Detector 3

Detector:
Viscometer
Measurement principle:
4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries
Transducers:
Firmware-based transducer overpressure protection
Detector volume:
17 µL/capillary
Differential pressure baseline noise:
0.3 Pa
Inlet pressure dynamic range:
100 kPa
Inlet pressure baseline noise:
0.01 kPa
Baseline drift:
<0.2 kPa/hr

Detector 4

Detector:
Diode-array-based UV/Vis spectrometer
Wavelength:
190 - 900 nm
Number of wavelengths:
1024
Wavelength accuracy:
<1 nm
Wavelength resolution:
0.6 nm
Cell volume:
7.5 µL
Path length:
10 mm
Baseline noise:
2 x 10-5 AU
Baseline drift:
5 x 10-4 AU/hr

Measurement type 1

Measurement type:
Absolute molecular weight
Measurement range:
200 - >107 g/mol
Minimum quantifiable mass:
100 ng of 100kDa molecular weight polystyrene in THF
Measurement principle:
Light scattering (LALS or RALS)

Measurement type 2

Measurement type:
Intrinsic viscosity
Minimum quantifiable mass:
1 µg of 100kDa molecular weight polystyrene in THF
Measurement principle:
4-capillary Wheatstone bridge

Measurement type 3

Measurement type:
Concentration
Minimum quantifiable mass:
100 ng of 100kDa molecular weight polystyrene in THF
Measurement principle:
Differential refractive index detection

Operating environment

Temperature:
15 - 30 °C

Software

Minimum computer specification:
Windows 7 Professional 64 bit, 4th Gen Intel(R) Core I7 Processor (Quad Core HT, 3.4 GHz Turbo, w/ HD Graphics), 8 GB 1600 MHz DDR3 memory, 500 GB 3.5 inch SATA (7200 RPM) HDD, Full-HD monitor

Accessories

OMNISEC system

OMNISEC RESOLVE Combined pump, degasser, autosampler and column oven, for high quality GPC/SEC and accurate molecular weight measurement. Image OMNISEC RESOLVE combines with OMNISEC REVEAL to form a complete OMNISEC GPC/SEC solution for the measurement of absolute molecular weight, intrinsic viscosity and other parameters. It combines an integrated pump, degasser, autosampler and column oven in a single advanced separations unit. More information
OMNISEC REVEAL The ultimate in integrated advanced detection for GPC/SEC Image Molecular weight, intrinsic viscosity and numerous other parameters can be measured with OMNISEC REVEAL, the world’s most advanced integrated GPC/SEC multi-detector available. It can be added to any existing conventional GPC/SEC system or used in conjunction with OMNISEC RESOLVE to form a complete GPC/SEC solution, to offer comprehensive molecular characterization of synthetic and natural polymers, as well as proteins. More information
OMNISEC software Powerful, agile, easy to use GPC/SEC software for OMNISEC instrument control, data acquisition, analysis and reporting Image OMNISEC software is an integrated solution for the advanced analysis of proteins and polymers by GPC/SEC, and is specifically designed for control of OMNISEC RESOLVE and OMNISEC REVEAL. Simplified workflows improve productivity and help you to maximize the information and efficiency of your experiments. Simply load your samples and the software controls all the sequencing, enabling truly automatic unattended operation. More information
GPC and SEC Columns GPC/SEC columns for all aqueous and organic applications Image A wide range of columns for analytical GPC/SEC of synthetic and natural polymers and proteins. More information
GPC and SEC Standards A range of molecular weight standards for different applications of GPC/SEC Image Malvern offers a wide range of molecular weight standards for GPC/SEC for conventional and universal calibration as well as light scattering. More information

Support

When you purchase a Malvern product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Some customers just need to ask an occasional question or to download a software upgrade, whilst others require a comprehensive support contract with priority call out and fixed cost of ownership. Whatever your situation Malvern will provide the right support for you.

Help Desk

  • Telephone and email support.
  • Operators who take responsibility for your call and ensure your query is answered. 

Training

  • On Site training courses
  • Classroom training
  • E-learning

Resource center

  • Application notes
  • Technical notes
  • On demand presentations
  • Live webinars

Software downloads

  • A simple mechanism for receiving updates and new features

Platinum and Gold Service Contracts

  • Protection from the cost of downtime
  • No time lost in obtaining help when you need it
  • Fixed cost of ownership

Bronze Service Agreements

  • Protect the value of your investment
  • Reduce the risk of downtime
  • Protect the validity of your data
 
 

Resource center search

Free access to exclusive content including webinars, presentations, application notes, technical notes, whitepapers and more. Enter your search term in the box and use the drop down boxes to filter the results.

Reset filters
Items shown: 5, 10, 20, ALL
Sort by: Date or Alphabetical
Previous page Showing results 1 - 5 of 23 Next page
Anaysis of PLGA Molecular Weight and Structure by the latest Adavanced Multi-Detector GPC Systems Poster (English)

Different samples of commercially available PLGA were analyzed to compare their absolute molecular weight from light scattering to those quoted with the product. Additionally, the Mark-Houwink plots of different examples containing different ratios o...


Keywords: Polymer solutions English Poster Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography OMNISEC 

Characterizing polymers: Techniques for the measurement of molecular weight, molecular size and branching. Whitepaper (English)

A review of the methods available for measuring the key characteristics of polymers focusing on the benefits and value of gel permeation/size exclusion chromatography (GPC/SEC). Much of the paper talks exclusively about polymers, however many of the ...


Keywords: Polymer solutions English Whitepaper Zetasizer range Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers, plastics and rubbers Adhesives and sealants Paints, inks and coatings Food additives Polysaccharides OMNISEC 

Compositional insights for protein mixtures: a case study of a semi-pure β-amylase extract Application Note (English)

In this application note, we describe the analysis of a semi-purified protein mixture containing β-amylase.  Step-by-step, we show how different properties of the proteins elicit a specific response in each detector and how the coupling this data can...


Keywords: English Application Note Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Protein aggregation Proteins Biopharmaceutical development OMNISEC 

OMNISEC system user manual (English) User Manual (English)

User manual for the OMNISEC system


Keywords: English User Manual OMNISEC 

OMNISEC system basic guide (English) User Manual (English)

Basic user guide for the OMNISEC system. This manual includes a system overview, instrument specification, regulatory statements, site requirements, health and safety information and maintenance procedures


Keywords: English User Manual OMNISEC 
Previous page Showing results 1 - 5 of 23 Next page

Glossary

Request a demo Request a quote Contact sales Register now
 
Download brochure