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Malvern’s OMNISEC is a complete Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) solution consisting of systems, detectors and software. It includes OMNISEC RESOLVE, the integrated separations module, OMNISEC REVEAL, the integrated multi-detector module and OMNISEC software for advanced GPC/SEC analysis.

Overview

In OMNISEC, Malvern's expertise has enabled the development of the most sensitive and accurate multi-detector GPC/SEC system available for the characterization of synthetic and natural polymers, and proteins.

OMNISEC RESOLVE combines an integrated pump, degasser, autosampler and column oven in a single advanced separations unit.

OMNISEC REVEAL is the most advanced integrated GPC/SEC multi-detector available, incorporating refractive index, UV/Vis absorbance, light scattering and intrinsic viscosity detectors.

  • Optimized isocratic pump and improved degassing efficiency in OMNISEC RESOLVE offers unrivalled flow rate stability to reduce baseline noise in all detectors.
  • Integrated column oven in OMNISEC RESOLVE maintains a stable separation temperature, all the way up to 65°C.
  • Autosampler with zero injection volume overhead mode prevents wastage of your most precious samples, and can cool fragile samples to 4°C to protect them from aggregation and degradation.
  • OMNISEC REVEAL's light scattering detector is the most sensitive available enabling measurements of molecular weights as low as 200 Da and injection masses as low as 100 ng.
  • The self-balancing differential viscometer in OMNISEC REVEAL has user exchangeable capillaries to make it more robust and usable than any other available.
  • Combine data from multiple detectors to calculate hydrodynamic radius (Rh), radius of gyration (Rg), and Mark-Houwink parameters.

How it works

Dissolved gasses are removed from the mobile phase as the pump draws it through the degasser before sending it onwards to the autosampler and column oven. The autosampler draws sample from either a vial or a microtiter plate and injects it into the mobile phase which then continues to the column. As the dissolved sample flows through the porous column matrix, the individual molecules diffuse in and out of the pores. Since larger molecules are excluded from more pores, they will travel through the column faster and elute earlier while the smaller molecules elute later. The result is a separation by hydrodynamic size for measurement by the detectors.

Specification

OMNISEC RESOLVE

Pump flow rate:
0.005 - 10 mL/min
Flow rate accuracy:
±1%
Pressure range:
0 - 5000 PSI (34.5 MPa)
Pulsation:
0.15% @ 1 mL/min in water
Precision:
0.25% RSD
Dimensions (W, D, H):
420, 640, 890 mm
Weight:
62 kg
Software:
OMNISEC software v10 (or later)
Data acquisition rate:
100 Hz
Patents:
US 14/599,033 "Continuous back seal wash"

AUTOSAMPLER

Capacity:
Up to 192
Sample container types:
HPLC vials or 96-well microtiter plates
Injection volume:
1 - 300 µL
Injection volume accuracy:
>99.5%
Injection volume precision:

<0.3% RSD in full loop mode

<0.5% RSD in partial loop mode

<1% RSD in µL pickup mode

Injection overhead volume:
0 µL in µL pickup mode
Syringe volume:
250 µL standard
Temperature control range:
4 - 60 °C

COLUMN OVEN

Column capacity:
6 x analytical (1 x Tricorn 10/300 GL)
Temperature control range:
20 - 65 °C

OMNISEC REVEAL

Dimensions (W, D, H):
420, 640, 600 mm
Weight:
40 kg
Detector temperature range:
20 - 65 °C
Software:
OMNISEC software v10 (or later)
Data acquisition rate:
100 Hz
Patents:

US20140060162A1 & EP2619543Ba "Modular capillary bridge viscometer"

US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"

Detector 1

Detector:
Light scattering
Measurement principle:
RALS 90° angle, LALS 7° angle
Light source:
50 mW, 640 nm laser
Cell volume:
18 µL
Dynamic range:
2500 mV
Baseline noise:
<0.1 mV
Baseline drift:
<0.2 mV/hr

Detector 2

Detector:
Differential refractive index
Measurement principle:
Deflection
Cell volume:
12 µL
Dynamic range:
±2.5 x 10-4 RIU
Baseline noise:
<10-7 RIU
Baseline drift:
<3 x 10-7 RIU/hr

Detector 3

Detector:
Viscometer
Measurement principle:
4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries
Transducers:
Firmware-based transducer overpressure protection
Detector volume:
17 µL/capillary
Differential pressure baseline noise:
0.3 Pa
Inlet pressure dynamic range:
100 kPa
Inlet pressure baseline noise:
0.01 kPa
Baseline drift:
<0.2 kPa/hr

Detector 4

Detector:
Diode-array-based UV/Vis spectrometer
Wavelength:
190 - 900 nm
Number of wavelengths:
1024
Wavelength accuracy:
<1 nm
Wavelength resolution:
0.6 nm
Cell volume:
7.5 µL
Path length:
10 mm
Baseline noise:
2 x 10-5 AU
Baseline drift:
5 x 10-4 AU/hr

Measurement type 1

Measurement type:
Absolute molecular weight
Measurement range:
200 - >107 g/mol
Minimum quantifiable mass:
100 ng of 100kDa molecular weight polystyrene in THF
Measurement principle:
Light scattering (LALS or RALS)

Measurement type 2

Measurement type:
Intrinsic viscosity
Minimum quantifiable mass:
1 µg of 100kDa molecular weight polystyrene in THF
Measurement principle:
4-capillary Wheatstone bridge

Measurement type 3

Measurement type:
Concentration
Minimum quantifiable mass:
100 ng of 100kDa molecular weight polystyrene in THF
Measurement principle:
Differential refractive index detection

Operating environment

Temperature:
15 - 30 °C

Software

Minimum computer specification:
Windows 7 Professional 64 bit, 4th Gen Intel(R) Core I7 Processor (Quad Core HT, 3.4 GHz Turbo, w/ HD Graphics), 8 GB 1600 MHz DDR3 memory, 500 GB 3.5 inch SATA (7200 RPM) HDD, Full-HD monitor

Accessories

OMNISEC system

OMNISEC RESOLVE Combined pump, degasser, autosampler and column oven, for high quality GPC/SEC and accurate molecular weight measurement. Image OMNISEC RESOLVE forms part of a complete OMNISEC GPC/SEC solution. It combines an integrated pump, degasser, autosampler and column oven in a single advanced separations unit. More information
OMNISEC REVEAL The ultimate in integrated advanced detection for GPC/SEC Image Incorporating refractive index, UV/Vis absorbance, light scattering and intrinsic viscosity detectors, OMNISEC REVEAL is the most advanced integrated GPC/SEC multi-detector available. It offers comprehensive molecular characterization of synthetic and natural polymers as well as proteins. More information
OMNISEC software Powerful, agile, easy to use GPC/SEC software for OMNISEC instrument control, data acquisition, analysis and reporting Image OMNISEC software is an integrated solution for the advanced analysis of proteins and polymers by GPC/SEC, and is specifically designed for control of OMNISEC RESOLVE and OMNISEC REVEAL. Simplified workflows improve productivity and help you to maximize the information and efficiency of your experiments. Simply load your samples and the software controls all the sequencing, enabling truly automatic unattended operation. More information
GPC and SEC Columns GPC/SEC columns for all aqueous and organic applications Image A wide range of columns for analytical GPC/SEC of synthetic and natural polymers and proteins. More information
GPC and SEC Standards A range of molecular weight standards for different applications of GPC/SEC Image Malvern offers a wide range of molecular weight standards for GPC/SEC for conventional and universal calibration as well as light scattering. More information

Support

When you purchase a Malvern product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Some customers just need to ask an occasional question or to download a software upgrade, whilst others require a comprehensive support contract with priority call out and fixed cost of ownership. Whatever your situation Malvern will provide the right support for you.

Help Desk

  • Telephone and email support.
  • Operators who take responsibility for your call and ensure your query is answered. 

Training

  • On Site training courses
  • Classroom training
  • E-learning

Resource center

  • Application notes
  • Technical notes
  • On demand presentations
  • Live webinars

Software downloads

  • A simple mechanism for receiving updates and new features

Platinum and Gold Service Contracts

  • Protection from the cost of downtime
  • No time lost in obtaining help when you need it
  • Fixed cost of ownership

Bronze Service Agreements

  • Protect the value of your investment
  • Reduce the risk of downtime
  • Protect the validity of your data
 
 

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The use of triple detection GPC to determine absolute molecular and structural information about low molecular weight heparins Poster (English)

In this work three commercially available Low Molecular Weight Heparin samples (Enoxaparin, Tinzaparin and Dalteparin) are measured by triple-detection GPC and any differences identified. In addition, structural comparisons will be made with a commer...


Keywords: English Poster Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Polysaccharides Pharmaceutical development OMNISEC 

Understanding the effect of molecular weight and structure on the performance characteristics of cellulose derivatives using OMNISEC Application Note (English)

A variety of aqueous (water-soluble) cellulose derivatives were characterized using the OMNISEC triple detection GPC/SEC system which revealed subtle, yet distinct differences between them. The differences described in this application note could be ...


Keywords: Polymer solutions English Application Note Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers from renewable sources Pharmaceutical development Excipients and coatings OMNISEC 

Characterization of IgG monomers & their aggregates Application Note (English)

Two antibody samples were characterized using column calibration and multi-detection SEC, and the results compared to show how multi-detection SEC provides more accurate data and a more complete characterization of the protein mixtures under study.


Keywords: English Application Note Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Protein aggregation Proteins Biopharmaceutical development Antibodies and derivatives OMNISEC 

The Mark–Houwink plot – Differentiation of Branching and Composition Application Note (English)

Using OMNISEC, we will show how to separate the effects of a structural difference induced by a compositional change to a polymer (e.g. substitution) and the structural change induced by polymer chain branching.


Keywords: English Application Note Molecular structure Molecular size Molecular weight OMNISEC 

GPC/SEC analysis of polylactic acid (PLA) and poly(lactic-co-glycolic acid) (PLGA) using OMNISEC Application Note (English)

In this application note, a selection of different PLA and PLGA polymer samples were analyzed on Malvern's OMNISEC GPC/SEC system, which combines multiple detectors to provide information about structure and solution properties.


Keywords: Polymer solutions English Application Note Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers from renewable sources Specialty and novel polymers Polymer recycling OMNISEC 
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