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The dual capability Zetasizer µV is dedicated to the measurement of proteins, and uses dynamic light scattering (DLS) to measure size, and static light scattering (SLS) to measure molecular weight.

Overview

The Zetasizer µV is a highly sensitive dual function light scattering detector. It can be used as a RALS detector with any GPC/SEC system, including Viscotek, to give absolute size (by DLS) and absolute molecular weight (by SLS). Alternatively, in cuvette mode, it is suitable for the detection of aggregates and for monitoring aggregate formation using DLS. The Zetasizer µV can be connected to any GPC/SEC system and concentration detector using an 8µl quartz flow cell and standard chromatography tubing.  This allows the measurement of absolute size, absolute molecular weight, aggregation, and conjugation (when used with a second concentration detector).

In cuvette mode, batch measurements of particle and molecular size (by DLS) can be made by simply removing the flow cell from the unit and inserting a standard cuvette containing the sample.

  • Switch in seconds from chromatography detector to cuvette mode.
  • Accurate and repeatable results using precise temperature control.
  • Sensitivity – 0.1mg/mL 15kDa protein (Lysozyme).
  • Only 2µL sample required in cuvette mode to preserve sample.
  • 8µL flow cell for chromatography mode ensures minimal band broadening to preserve the resolution of the separation.
  • Connect to any Viscotek system for full software integration and truly automated operation
  • Connect to any third party SEC system using OmniSEC software and OmniFACE interface.
  • Automated temperature trends for aggregation temperature determination.

How it works

The Zetasizer µV incorporates two techniques that can be used in both chromatography detector mode and cuvette mode.
Dynamic Light Scattering is used to measure particle and molecule size. This technique measures the diffusion of protein molecules moving under Brownian motion, and converts this to size and a size distribution using the Stokes-Einstein relationship.

Static Light Scattering is used to determine molecular weight. When used as a SEC detector, the light scattering intensity in combination with the signal from a concentration detector enables the absolute molecular weight to be calculated. In cuvette mode, measurements from a few different concentrations are combined to draw a Debye plot. From this a molecular weight and second virial coefficient can be calculated.

Specification

Measurement type 1

Measurement type:
Particle size, molecular size.
Measurement range:
0.15nm – 1.0 microns* (radius)
Measurement principle:
Dynamic Light Scattering
Measurement angle:
90°
Minimum sample volume:
2µL
Accuracy:
Better than +/-2% on NIST traceable latex standards
Precision / Repeatability:
Better than +/-2% on NIST traceable latex standards

Measurement type 2

Measurement type:
Molecular weight as SEC detector
Measurement range:
<1kDa – 5MDa
Measurement principle:
Static Light Scattering
Accuracy:
+/- 5% typical
Flow cell volume:
8µl

Measurement type 3

Measurement type:
Molecular weight as batch detector
Measurement range:
<1kDa – 5MDa
Measurement principle:
Static Light Scattering using Debye plot
Minimum sample volume:
2µL (3-5 sample concentrations required)
Accuracy:
+/- 10% typical

General

Temperature control range:
2°C - 90°C +/-0.1.**
Light source:
Laser 830nm, Max 60mW
Laser safety:
Class 1
Power:
100VA

Weight and dimensions

Dimensions (W, D, H):
350mm x 410mm x 170mm
Weight:
14kg

Operating environment

Operating temperature (°C):
15°C – 40°C
Humidity:
35% - 80% non-condensing

Zetasizer µV

*:
Maximum range, sample dependent.
**:
At 25°C

Accessories

Cell and cuvette options

Quartz flow cell (ZMV1008) Add flow capability for size measurements to the Zetasizer. Measure size and intensity with MPT-2 autotitrator. Connect to an SEC system for use as a DLS detector, to measure protein size and molecular weight. Low 8µL internal volume.
Low-volume quartz batch cuvette (ZMV1002) Low-volume quartz batch cuvette for size and molecular weight measurements. Compatible with aqueous and non-aqueous solvents and dispersants. Absolute size measurement. Measure molecular weight and second virial coefficient. Minimum sample volume 2 microliters.
Quartz batch cuvette (ZMV1012) Quartz cuvette for batch measurements. Compatible with aqueous and non-aqueous solvents and dispersants. Absolute size measurement. Measure molecular weight and second virial coefficient. Minimum sample volume 12 microliters.
12mm Square Polystyrene Cuvettes (DTS0012) Disposable polystyrene cuvettes. 100 disposable cells complete with 100 stoppers to avoid cross contamination. For size measurements using aqueous dispersants. Compatible with zeta potential measurements using the dip cell. Visit store to buy now
 
 

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Controlling the dispersion or flocculation of colloidal suspensions through zeta potential Webinar - Live (English)

Many large scale industrial processes (paper production, water treatment and dewatering processes) require de-stabilization or flocculation of dispersed particulates. For other industries and products such as foods, cosmetics, paints and pharmaceutic...

Product:
Zetasizer range
Date:
October 20 2016
Language:
English


Applying complementary particle techniques for assessing comparability and biosimilarity of therapeutic products Webinar - Recorded (English)

Significant advances have been made in analytical technology for the characterization and identification of particles present in pharmaceutical products. With these advances comes a tremendous amount of new data with which to characterize biologics, ...

Product:
Archimedes,Zetasizer range,Viscotek SEC-MALS,Morphologi G3-ID,NanoSight range
Date recorded:
September 20 2016
Language:
English


Zeta potential - An important parameter for controlling formulation stability and product performance Webinar - Recorded (English)

Cosmetics, paints, inks, paper, drug delivery, personal care, food or intra-lipid emulsions are made up of multi component chemistries. Most products go through a stage where different phases co-exist such as emulsions, suspensions or dispersions and...

Product:
Zetasizer range
Date recorded:
September 8 2016
Language:
English


How multi-detector GPC/SEC can advance our understanding of drug delivery polymers Webinar - Recorded (English)

Correctly targeting a drug product’s delivery location and defining its release profile are critical challenges and the subject of a great deal of research. The role of polymers in drug delivery applications is growing, with particular emphasis on ta...

Product:
Viscotek range,OMNISEC,Zetasizer range
Date recorded:
September 6 2016
Language:
English


How Do I Verify My Zeta Potential Instrument? FAQ

Electrophoretic light scattering (ELS) or zeta potential instruments use first principles in their measurement protocol. They cannot therefore, be calibrated. However, they can be verified that they are operating correctly, by measuring a suitable zeta potential standard.


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Controlling the dispersion or flocculation of colloidal suspensions through zeta potential
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