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The dual capability Zetasizer µV is dedicated to the measurement of proteins, and uses dynamic light scattering (DLS) to measure size, and static light scattering (SLS) to measure molecular weight.
The Zetasizer µV is a highly sensitive dual function light scattering detector. It can be used as a RALS detector with any GPC/SEC system, including Viscotek, to give absolute size (by DLS) and absolute molecular weight (by SLS). Alternatively, in cuvette mode, it is suitable for the detection of aggregates and for monitoring aggregate formation using DLS. The Zetasizer µV can be connected to any GPC/SEC system and concentration detector using an 8µl quartz flow cell and standard chromatography tubing. This allows the measurement of absolute size, absolute molecular weight, aggregation, and conjugation (when used with a second concentration detector).
In cuvette mode, batch measurements of particle and molecular size (by DLS) can be made by simply removing the flow cell from the unit and inserting a standard cuvette containing the sample.
The Zetasizer µV incorporates two techniques that can be used in both chromatography detector mode and cuvette mode.
Dynamic Light Scattering is used to measure particle and molecule size. This technique measures the diffusion of protein molecules moving under Brownian motion, and converts this to size and a size distribution using the Stokes-Einstein relationship.
Static Light Scattering is used to determine molecular weight. When used as a SEC detector, the light scattering intensity in combination with the signal from a concentration detector enables the absolute molecular weight to be calculated. In cuvette mode, measurements from a few different concentrations are combined to draw a Debye plot. From this a molecular weight and second virial coefficient can be calculated.
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The stability of a protein sample and its tendency to aggregate is explored using a combination of SEC-MALS and DLS
This webinar discusses how Malvern’s protein SEC measurement capabilities within the Viscotek range have developed and improved in the last year. We also introduce the new features that are included in the latest release of OmniSEC, including:
This seminar discusses the total characterization of a number of model biomolecules using Dynamic Light Scattering, Electrophoretic Light Scattering and Multi-Angle Light Scattering combined with SEC. Data available from these techniques include size...
Biopharmaceutical impurities have long been associated with undesirable clinical reactions. It is essential, therefore, that the purity profiles of such drugs are characterized with the most sensitive analysis methods available. Dynamic light scatte...
This presentation introduces the main particle characterization tehniques currently used in industry and academia, the basic theoretical concepts of particle size analysis as well as aspects of its practical application.
(Webinar - Recorded)
The Zetasizer Nano is so easy to use. All my students can familiarise themselves with it very quickly. As a result, we are purchasing a second instrument to ensure we have enough capacity for our expanding research programme in this area.
Department of Chemistry, at The University of Sheffield
We very much value the customer service and training opportunities from Malvern. In particular, the rapid diagnosis of any issues through direct internet connection with the Malvern customer service team has prevented us from suffering production downtime.
Dr. Cordula Grüttner
micromod Partikeltechnologie GmbH
The Malvern systems have proved highly effective, allowing us to further demonstrate the stability and purity of our products and how this may be translated into improving liquid biopharmaceutical formulations.
We’ve always been really happy with Malvern and while we’ve tried other companies, the necessary expertise was not there.
the IN-CTSI (Indiana-Clinical Translational Sciences Institute)
Nanoparticle size is an important criterion for our formulations and we routinely measure this with the Zetasizer Nano.
My students have always had great things to say about the Zetasizer Nano. They say it’s very easy to use. The software is very user-friendly and it can accommodate particles of various different sizes, so I have never had reason to evaluate another particle sizing instrument.
Dr. Albena Ivanisevic
Department of Materials Science and Engineering at North Carolina State University
On-line zeta potential measurement is helping us to achieve a more cost-efficient water facility.
Here at IU’s Nanocharacterization facility, the Malvern Zetasizer Nano is the ‘go to’ instrument for characterization of assembled product.
Dr Stella Aniagyei
Indiana University’s (IU) Molecular and Cellular Biochemistry Department
The big plus of an on-line instrument is real-time monitoring, the value of which was really brought home to us during a recent treatment event. The on-line data we collected during the event gave us new insight into a modified operating scheme that we’re now capitalizing on.
The [Zetasizer Nano] instrument is robust, easy to use and data acquisition is straightforward. It is as close to ‘student proof’ as it is possible to be!
Dr Krystelle Mafina
Queen Mary University
The technical support that we’ve received has been a deciding factor in our decision to continue to work with Malvern. Malvern systems also provide the cutting-edge performance and reliability that we need.
Domino Printing Sciences plc
The Zetasizer Nano has reliably delivered a substantial portion of data and continues to help drive our research.
As part of the multi-disciplinary lab at QMUL, materials and biomaterials research groups throughout the university, from engineers and chemists through to biologists and physicists, are benefiting from access to analysis using the Zetasizer Nano.
We’ve been running the Zetasizer APS continuously since 2012 and the real beauty is that we simply load the samples in multiwell plates, leave it to run, even overnight, and go back and pick up the results.
Dr Maria Håkansson
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