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The dual capability Zetasizer µV is dedicated to the measurement of proteins, and uses dynamic light scattering (DLS) to measure size, and static light scattering (SLS) to measure molecular weight.
The Zetasizer µV is a highly sensitive dual function light scattering detector. It can be used as a RALS detector with any GPC/SEC system, including Viscotek, to give absolute size (by DLS) and absolute molecular weight (by SLS). Alternatively, in cuvette mode, it is suitable for the detection of aggregates and for monitoring aggregate formation using DLS. The Zetasizer µV can be connected to any GPC/SEC system and concentration detector using an 8µl quartz flow cell and standard chromatography tubing. This allows the measurement of absolute size, absolute molecular weight, aggregation, and conjugation (when used with a second concentration detector).
In cuvette mode, batch measurements of particle and molecular size (by DLS) can be made by simply removing the flow cell from the unit and inserting a standard cuvette containing the sample.
The Zetasizer µV incorporates two techniques that can be used in both chromatography detector mode and cuvette mode.
Dynamic Light Scattering is used to measure particle and molecule size. This technique measures the diffusion of protein molecules moving under Brownian motion, and converts this to size and a size distribution using the Stokes-Einstein relationship.
Static Light Scattering is used to determine molecular weight. When used as a SEC detector, the light scattering intensity in combination with the signal from a concentration detector enables the absolute molecular weight to be calculated. In cuvette mode, measurements from a few different concentrations are combined to draw a Debye plot. From this a molecular weight and second virial coefficient can be calculated.
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The stability of a protein sample and its tendency to aggregate is explored using a combination of SEC-MALS and DLS
This webinar discusses how Malvern’s protein SEC measurement capabilities within the Viscotek range have developed and improved in the last year. We also introduce the new features that are included in the latest release of OmniSEC, including:
This seminar discusses the total characterization of a number of model biomolecules using Dynamic Light Scattering, Electrophoretic Light Scattering and Multi-Angle Light Scattering combined with SEC. Data available from these techniques include size...
Biopharmaceutical impurities have long been associated with undesirable clinical reactions. It is essential, therefore, that the purity profiles of such drugs are characterized with the most sensitive analysis methods available. Dynamic light scatte...
This presentation introduces the main particle characterization tehniques currently used in industry and academia, the basic theoretical concepts of particle size analysis as well as aspects of its practical application.
(Webinar - Recorded)
As part of the multi-disciplinary lab at QMUL, materials and biomaterials research groups throughout the university, from engineers and chemists through to biologists and physicists, are benefiting from access to analysis using the Zetasizer Nano.
Dr Krystelle Mafina,
Queen Mary University,
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Malvern Instruments pursues a policy of continual improvement due to technical development. We therefore reserve the right to deviate from information, descriptions, and specifications in this publication without notice. Malvern Instruments shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance or use of this material.
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