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The dual capability Zetasizer µV is dedicated to the measurement of proteins, and uses dynamic light scattering (DLS) to measure size, and static light scattering (SLS) to measure molecular weight.

Overview

The Zetasizer µV is a highly sensitive dual function light scattering detector. It can be used as a RALS detector with any GPC/SEC system, including Viscotek, to give absolute size (by DLS) and absolute molecular weight (by SLS). Alternatively, in cuvette mode, it is suitable for the detection of aggregates and for monitoring aggregate formation using DLS. The Zetasizer µV can be connected to any GPC/SEC system and concentration detector using an 8µl quartz flow cell and standard chromatography tubing.  This allows the measurement of absolute size, absolute molecular weight, aggregation, and conjugation (when used with a second concentration detector).

In cuvette mode, batch measurements of particle and molecular size (by DLS) can be made by simply removing the flow cell from the unit and inserting a standard cuvette containing the sample.

  • Switch in seconds from chromatography detector to cuvette mode.
  • Accurate and repeatable results using precise temperature control.
  • Sensitivity – 0.1mg/mL 15kDa protein (Lysozyme).
  • Only 2µL sample required in cuvette mode to preserve sample.
  • 8µL flow cell for chromatography mode ensures minimal band broadening to preserve the resolution of the separation.
  • Connect to any Viscotek system for full software integration and truly automated operation
  • Connect to any third party SEC system using OmniSEC software and OmniFACE interface.
  • Automated temperature trends for aggregation temperature determination.

How it works

The Zetasizer µV incorporates two techniques that can be used in both chromatography detector mode and cuvette mode.
Dynamic Light Scattering is used to measure particle and molecule size. This technique measures the diffusion of protein molecules moving under Brownian motion, and converts this to size and a size distribution using the Stokes-Einstein relationship.

Static Light Scattering is used to determine molecular weight. When used as a SEC detector, the light scattering intensity in combination with the signal from a concentration detector enables the absolute molecular weight to be calculated. In cuvette mode, measurements from a few different concentrations are combined to draw a Debye plot. From this a molecular weight and second virial coefficient can be calculated.

Specification

Measurement type 1

Measurement type:
Particle size, molecular size.
Measurement range:
0.15nm – 1.0 microns* (radius)
Measurement principle:
Dynamic Light Scattering
Measurement angle:
90°
Minimum sample volume:
2µL
Accuracy:
Better than +/-2% on NIST traceable latex standards
Precision / Repeatability:
Better than +/-2% on NIST traceable latex standards

Measurement type 2

Measurement type:
Molecular weight as SEC detector
Measurement range:
<1kDa – 5MDa
Measurement principle:
Static Light Scattering
Accuracy:
+/- 5% typical
Flow cell volume:
8µl

Measurement type 3

Measurement type:
Molecular weight as batch detector
Measurement range:
<1kDa – 5MDa
Measurement principle:
Static Light Scattering using Debye plot
Minimum sample volume:
2µL (3-5 sample concentrations required)
Accuracy:
+/- 10% typical

General

Temperature control range:
2°C - 90°C +/-0.1.**
Light source:
Laser 830nm, Max 60mW
Laser safety:
Class 1
Power:
100VA

Weight and dimensions

Dimensions (W, D, H):
350mm x 410mm x 170mm
Weight:
14kg

Operating environment

Operating temperature (°C):
15°C – 40°C
Humidity:
35% - 80% non-condensing

Zetasizer µV

*:
Maximum range, sample dependent.
**:
At 25°C

Latest software update

7.11
(25/09/2014)
Download update

Accessories

Cell and cuvette options

Quartz flow cell (ZMV1008) Add flow capability for size measurements to the Zetasizer. Measure size and intensity with MPT-2 autotitrator. Connect to an SEC system for use as a DLS detector, to measure protein size and molecular weight. Low 8µL internal volume.
Low-volume quartz batch cuvette (ZMV1002) Low-volume quartz batch cuvette for size and molecular weight measurements. Compatible with aqueous and non-aqueous solvents and dispersants. Absolute size measurement. Measure molecular weight and second virial coefficient. Minimum sample volume 2 microliters.
Quartz batch cuvette (ZMV1012) Quartz cuvette for batch measurements. Compatible with aqueous and non-aqueous solvents and dispersants. Absolute size measurement. Measure molecular weight and second virial coefficient. Minimum sample volume 12 microliters.
12mm Square Polystyrene Cuvettes (DTS0012) Disposable polystyrene cuvettes. 100 disposable cells complete with 100 stoppers to avoid cross contamination. For size measurements using aqueous dispersants. Compatible with zeta potential measurements using the dip cell.
 
 

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Protein stability measurements amplified: Combining SEC-MALS and DLS to maximize data output Application Note (English)

The stability of a protein sample and its tendency to aggregate is explored using a combination of SEC-MALS and DLS



Powerful protein SEC made simple! Webinar - Recorded (English)

This webinar discusses how Malvern’s protein SEC measurement capabilities within the Viscotek range have developed and improved in the last year. We also introduce the new features that are included in the latest release of OmniSEC, including: Bio...

Product:
Viscotek SEC-MALS,Zetasizer µV
Date recorded:
September 30 2014
Language:
English

Keywords: English Gel Permeation Chromatography Size Exclusion Chromatography Webinar - Recorded Eastern Time Zetasizer µV Proteins Viscotek SEC-MALS 

Pushing the limits of Light Scattering Detection for Biotherapeutic Characterization Webinar - Recorded (English)

This seminar discusses the total characterization of a number of model biomolecules using Dynamic Light Scattering, Electrophoretic Light Scattering and Multi-Angle Light Scattering combined with SEC. Data available from these techniques include size...

Product:
Zetasizer Nano ZSP,Viscotek SEC-MALS,Zetasizer µV
Date recorded:
April 24 2014
Language:
English

Keywords: English Molecular size Molecular weight Particle size Dynamic Light Scattering Electrophoretic Light Scattering Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Webinar - Recorded GMT Time Zetasizer µV Protein aggregation Proteins Zetasizer Nano ZSP Protein mobility Biological drug characterization and development Viscotek SEC-MALS 

"Step into the Light" - how dynamic light scattering can improve the quality of your biopharmaceutical data Webinar - Recorded (English)

Biopharmaceutical impurities have long been associated with undesirable clinical reactions. It is essential, therefore, that the purity profiles of such drugs are characterized with the most sensitive analysis methods available. Dynamic light scatte...

Product:
Zetasizer µV,Zetasizer APS,Zetasizer Nano range
Date recorded:
January 21 2014
Language:
English

Keywords: English Molecular weight Particle size Dynamic Light Scattering Static Light Scattering Webinar - Recorded GMT Time Zetasizer APS Zetasizer Nano range Zetasizer µV Protein aggregation Proteins Biopharmaceutical development 

A basic guide to particle characterization Webinar - Recorded (English)

This presentation introduces the main particle characterization tehniques currently used in industry and academia, the basic theoretical concepts of particle size analysis as well as aspects of its practical application.

Product:
Mastersizer range,Morphologi G3,Sysmex FPIA-3000,Zetasizer APS,Zetasizer Nano range,Zetasizer µV
Date recorded:
August 19 2013
Language:
English

Keywords: English Mastersizer range Morphologi G3 Sysmex FPIA-3000 Particle shape Particle size Zeta potential Dynamic Light Scattering Electrophoretic Light Scattering Image analysis Laser diffraction Webinar - Recorded Greenwich Time Zetasizer APS Zetasizer Nano range Zetasizer µV 
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