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The Zetasizer Nano ZSP is the premium system in the Zetasizer range. It is used for the measurement of size, electrophoretic mobility of proteins, zeta potential of nanoparticles and surfaces, and optionally the microrheology of protein and polymer solutions.

The exceptional performance also enables the measurement of the molecular weight and second virial coefficient, A2, of macromolecules and kD, the DLS interaction parameter.

The system can also be used in a flow configuration to operate as a size detector for Size Exclusion Chromatography (SEC) or Field Flow Fractionation (FFF).


The Zetasizer Nano ZSP is the world’s highest performance system and particularly suitable for the characterization of proteins and nanoparticles where the highest sensitivity for size and zeta potential measurement is required. It includes a Protein Measurement option for protein mobility measurements.

The system incorporates a two angle particle and molecular size analyzer for the enhanced detection of aggregates and measurement of small or dilute samples, and samples at very low or high concentration using dynamic light scattering with ‘NIBS’ optics. The ZSP also incorporates a zeta potential analyzer that uses electrophoretic light scattering for particles, molecules and surfaces, and a molecular weight analyzer using static light scattering.

Using Non-Invasive Backscatter optics (NIBS) it has significantly better performance than systems using 90 degree scattering optics.

In addition, a microrheology option is available for measuring sample viscosity and viscoelastic properties.

The flow mode option enables the system to be connected to an SEC or an FFF system to use as a detector for the size of proteins or nanoparticles.

A choice of cuvettes are available, from disposable single-use to specific cells for viscous or high concentration samples or measuring the zeta potential of surfaces.

Parameters measured:
Particle and molecule size, translational diffusion, electrophoretic mobility, zeta potential of particles at high and low concentrations, viscosity and viscoelasticity of protein and polymer solutions, concentration, MW, A2, kD.

An optional accessory enables measurement of the zeta potential of solid surfaces.

  • Exceptional sensitivity for the zeta potential measurement of proteins and nanoparticles using patented M3-PALS.
  • Size measurement from 0.3nm (diameter) to 10 microns using patented NIBS (Non-Invasive Back Scatter) technology.
  • Zeta potential of surfaces using accessory cell.
  • Molecular weight measurement down to 980Da.
  • Microrheology option to measure viscosity and viscoelasticity.
  • Outstanding protein size measurement sensitivity, 0.1mg/mL (Lysozyme).
  • Sample concentrations from 0.1ppm to 40%w/v.
  • Built-in protein calculators, including protein charge, A2, kD, and molecular conformation.
  • A ‘Quality Factor’ and ‘Expert Advice System’ gives the confidence of having an expert at your shoulder.
  • 21CFR part 11 software option to enable compliance with ER/ES.
  • Research software option to give access to further features and analysis algorithms for the light scattering specialist.
  • Automation of measurements using an autotitrator option.
  • Chromatography detector capability to enable use as a size detector with GPC / SEC or FFF.
  • Optical filter option to improve measurements with fluorescent samples.

How it works

The Zetasizer Nano ZSP incorporates three techniques in a single compact unit, and has a range of options and accessories to optimize and simplify the measurement of different sample types.

Dynamic Light Scattering is used to measure particle and molecule size. DLS measures the diffusion of particles moving under Brownian motion, and converts this to size and a size distribution using the Stokes-Einstein relationship. Non-Invasive Back Scatter technology (NIBS) is incorporated to give the highest sensitivity simultaneously with the highest size and concentration range.

Measurement of size as a function of concentration enables the calculation of kD, the DLS interaction parameter.

The Microrheology option uses the DLS measurement of tracer particles to probe the structure of dilute polymer and protein solutions.

Laser Doppler Micro-electrophoresis is used to measure zeta potential. An electric field is applied to a solution of molecules or a dispersion of particles, which then move with a velocity related to their zeta potential. This velocity is measured using a patented laser interferometric technique called M3-PALS (Phase analysis Light Scattering). This enables the calculation of electrophoretic mobility and from this the zeta potential and zeta potential distribution.

A surface zeta potential accessory uses tracer particles to measure electro-osmosis close to a sample surface to calculate the zeta potential of the surface.

Static Light Scattering is used to determine the molecular weight of proteins and polymers. The scattering intensity of a number of concentrations of the sample is measured, and used to construct a Debye plot. From this the weight average molecular weight and second virial coefficient can be calculated, which provides a measure of protein solubility.

This technique is very demanding on the sensitivity and stability of the whole system, and requires that every element of the design is optimized to ensure accuracy and repeatability.

The software is designed to be versatile and rich in features without compromising ease of use. Standard Operating Procedures (SOP) simplify routine measurements, a quality factor gives a single place for confirmation of data quality, and an expert advice system is provided to assist with data interpretation.

The MPT-2 Autotitrator enables the study of the effect of changes in pH, conductivity, or any additive to be automated.

A range of disposable and reusable cells are available to optimize the measurement in terms of sample volume, concentration and flow measurement.

Other options include filters to improve the measurement of fluorescent samples and a viscometer to determine the sample viscosity to the accuracy required for the techniques used.


Particle size and molecular size

Measurement range:
0.3nm – 10.0 microns* (diameter)
Measurement principle:
Dynamic Light Scattering
Minimum sample volume:
Better than +/-2% on NIST traceable latex standards
Precision / Repeatability:
Better than +/-2% on NIST traceable latex standards
0.1mg/mL (Lysozyme)

Zeta potential and Protein Mobility

Measurement range:
3.8nm – 100 microns (diameter)*
Measurement principle:
Electrophoretic Light Scattering
Minimum sample volume:
150µL (20µL using diffusion barrier method)
0.12µ for aqueous systems using NIST SRM1980 standard reference material
1mg/mL (Lysozyme)

Molecular weight

Measurement range:
980Da – 20M Da*
Measurement principle:
Static Light Scattering using Debye plot
Minimum sample volume:
12µL (3-5 sample concentrations required)
+/- 10% typical

Microrheology (option)

Measurement principle:
Dynamic Light Scattering
Minimum sample volume:
12µL (DLS measurement only)


Temperature control range:
0°C - 90°C +/-0.1**
Light source:
He-Ne laser 633nm, Max 10mW
Laser safety:
Class 1
Dimensions (W, D, H):
320mm, 600mm, 260mm

Operating environment

10°C – 35°C
35% - 80% non-condensing


maximum range, sample dependent
at 25ºC
The Zetasizer Nano range is protected by the following patents: Non-Invasive Back Scatter (NIBS) EP884580, US6016195, JP11051843 High and Low Frequency Electrophoreses (M3) EP1154266, US7217350, JP04727064 Light Scattering Measurements using Simultaneous Detection EP2235501, CN102066901, JP2011523451, US20090251696 Surface Potential Determination in a Dip Cell WO2012172330



NanoSampler Automated particle size measurement for the Zetasizer Nano Image The Zetasizer NanoSampler is a sample delivery system that ensures highly precise and reproducible automated loading of samples into your Zetasizer Nano. The NanoSampler adds automation and unattended operation in a versatile, compact package, maximising the productivity of your Zetasizer Nano. While the benefits of automated operation are useful for all users, the NanoSampler is ideally suited for laboratories where a large number of measurements are required and reproducibility or multi-variate studies are important. More information
MPT-2 Simplify pH, conductivity and additive titrations on your Zetasizer Nano Image A cost effective accessory designed to automate the measurement of size and zeta potential as a function of pH, conductivity or any soluble additive. More information
SV-10 viscometer Viscosity measurement with speed, simplicity and accuracy. Image The SV-10 measures viscosity with the accuracy required for dynamic light scattering, for the conversion of diffusion speed to size, and electrophoretic mobility to zeta potential, in an extremely simple and rapid system. More information

Cell and cuvette options

Disposable folded capillary cell Eliminate sample cross contamination between zeta potential measurements using a completely disposable cell. Image The zeta potential of colloids and molecules is extremely sensitive to contamination from components of a previous sample used in a measurement cell. The use of a disposable cell completely eliminates this potential contamination issue. The folded capillary cell provides the benefits of the accuracy of measurement of a capillary cell with the ease of use of a disposable cell. Although, disposable, including the electrodes, for low conductivity samples each cell can be used for hundreds of measurements if required. The cell can be used with the autotitrator, where multiple measurements are routinely done over a wide pH range.
Universal 'dip' cell kit Reusable solvent resistant cell. Image The dip cell is used for the measurement of zeta potential of samples in aqueous and non-aqueous dispersants, and is designed to be cleaned and reused. It is for batch measurements using standard polystyrene or glass cuvettes.
High concentration zeta potential cell kit Save time and effort by measuring zeta potential with little or no dilution. Image The high concentration cell enables the measurement of the zeta potential of samples with little or no dilution in any Zetasizer Nano system that measures zeta potential. The cell has a flow configuration so can be used for stop-flow size and zeta potential measurements using an MPT-2 autotitrator as well as batch measurements.
Surface zeta potential accessory Extend the application of the Zetasizer Nano to measure the zeta potential of material surfaces. Image The characterization of surfaces and interfaces is critical in a wide range of industries including consumer products, specialty chemicals, pharmaceuticals and life sciences. In each of these application areas, the interaction of molecules and particles with surfaces can influence the material properties of the end product. The surface zeta potential accessory enables the measurement of the zeta potential of flat surfaces such as filter paper, silica, polymers and bone, using a standard Zetasizer Nano system.
Quartz flow cell (ZEN0023) Add flow capability for size measurements to the Zetasizer. Measure size and intensity with MPT-2 autotitrator. Connect to an SEC system to use as a DLS detector.
12mm Glass cell (PCS1115) Glass cuvette for use with the dip cell. For size and zeta potential measurements using the ‘dip’ cell. Compatible with aqueous and non-aqueous solvents and dispersants.
12mm Square Polystyrene Cuvettes (DTS0012) Disposable polystyrene cuvettes. 100 disposable cells complete with 100 stoppers to avoid cross contamination. For size measurements using aqueous dispersants. Compatible with zeta potential measurements using the dip cell.
12mm square glass cell (PCS8501) Glass cuvette for batch measurements. For size and molecular weight measurements. Compatible with aqueous and non-aqueous solvents and dispersants.
Disposable Solvent Resistant Micro Cuvette (ZEN0040) Disposable plastic micro cuvette, for size measurement at 173° (NIBS) optics only. 100 x 40µL cuvettes with 100 caps to avoid cross contamination. Compatible with aqueous samples and a range of other solvents: Acetone, Benzaldehyde, Butanone, Dioxane, DMF, Ethyl acetate, Isopropanol, plus various acids and bases.
Low-volume quartz batch cuvette (ZEN2112) Low-volume quartz batch cuvette for size and molecular weight measurements. Compatible with size and molecular weight measurements with aqueous and non-aqueous solvents and dispersants. Minimum sample volume 12 microliters. Maximum volume 45 microliters.
ZEN0118 Disposable low volume cuvette for size measurement with 90° optics only. 100 x 50µL cuvettes with 100 caps to avoid cross contamination. Compatible with aqueous dispersants.

Factory fitted options

Narrow band filters Useful where the sample fluoresces. Eliminates scattering due to fluorescence to improve the signal to background ratio.
Degasser for MPT-2 autotitrator Titrants used by the MPT-2 titrator need regular degassing, ideally before each titration. Degassing is required to remove bubbles that can affect the volume of titrant dispensed. This is particularly important close to pH 7 where low volumes of titrant are required. The degasser is a highly recommended ‘Fit and forget’ accessory that continuously degasses titrants which improves the speed of titrations and the accuracy of reaching the target pH. This gives more evenly spaced pH points.


When you purchase a Malvern product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Some customers just need to ask an occasional question or to download a software upgrade, whilst others require a comprehensive support contract with priority call out and fixed cost of ownership. Whatever your situation Malvern will provide the right support for you.

Help Desk

  • Telephone and email support.
  • Operators who take responsibility for your call and ensure your query is answered. 


  • On Site training courses
  • Classroom training
  • E-learning

Resource center

  • Application notes
  • Technical notes
  • On demand presentations
  • Live webinars

Software downloads

  • A simple mechanism for receiving updates and new features

Platinum and Gold Service Contracts

  • Protection from the cost of downtime
  • No time lost in obtaining help when you need it
  • Fixed cost of ownership

Bronze Service Agreements

  • Protect the value of your investment
  • Reduce the risk of downtime
  • Protect the validity of your data

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Using Automated Light Scattering Measurements to Support Predictive Protein Formulation Screening Article (English)

High concentration, low-viscosity, stable therapeutic protein formulations are needed to enable low-volume parental administration. This article explores how a fully automated light scattering system supports the rapid screening of small volume sampl...

Keywords: Emulsions and creams English Article Bohlin Gemini 2 Kinexus pro+ Particle size Rheology and viscosity Zeta potential Dynamic Light Scattering Electrophoretic Light Scattering Rheometry - rotational Personal care Zetasizer Nano S Zetasizer Nano ZS Zetasizer Nano ZSP 

Analytical strategies for ink formulation Whitepaper (English)

This white paper offers practical guidance on using a range of analytical techniques, including rheology, particle size and zeta potential measurement to assist in the formulation of Inkjet inks. 

Keywords: Films and coatings English Whitepaper Kinexus range Mastersizer range Particle size Rheology and viscosity Dynamic Light Scattering Laser diffraction Rheometry - rotational Pigments and toners Paints, inks and coatings Zetasizer Nano range m-VROCi Rheometry - microfluidic flow 

Nanoparticle size characterization: Tips and Tricks Webinar - Recorded (English)

Nanoparticles are defined by their size. In this presentation different sizing techniques are compared, with a focus on experimental tips and tricks to get maximum value from sizing techniques such as dymamic light scattering with the Malvern Zetasiz...

Zetasizer Nano range,Viscotek systems
Date recorded:
November 11 2014

Keywords: English Dynamic Light Scattering Webinar - Recorded Eastern Time Zetasizer Nano range Viscotek systems 

Nanobubbles - separating the fact from fiction Webinar - Recorded (English)

Ultra-Fine (nano) bubbles have garnered increasing interest over the last few years as they open up the potential for improvements in a variety of applications from cleaning to agriculture. However they are still somewhat a mystery with respect to th...

Archimedes,Zetasizer Nano range,NanoSight range
Date recorded:
October 30 2014

Keywords: English Particle concentration Particle size Dynamic Light Scattering Webinar - Recorded Eastern Time Zetasizer Nano range Resonant mass measurement Archimedes NanoSight range Nanobubbles Nanoparticle Tracking Analysis 

How to characterize and optimize inkjet inks Part 2 Webinar - Recorded (English)

Achieving product stability is a critical aspect of ink formulation. Modern inks are typically colloidal suspensions of multiple components in an organic or aqueous continuous phase. The functionality of the finished product relies on maintaining sus...

Zetasizer Nano range,Mastersizer range
Date recorded:
October 21 2014

Keywords: Suspensions, slurries and pastes English Mastersizer range Particle size Rheology and viscosity Zeta potential Laser diffraction Pigments and toners Paints, inks and coatings Webinar - Recorded Eastern Time Zetasizer Nano range 
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