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Dynamic light scattering (DLS), sometimes referred to as Quasi-Elastic Light Scattering (QELS), is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1nm.

Typical applications of dynamic light scattering are the characterization of particles, emulsions or molecules, which have been dispersed or dissolved in a liquid. The Brownian motion of particles or molecules in suspension causes laser light to be scattered at different intensities. Analysis of these intensity fluctuations yields the velocity of the Brownian motion and hence the particle size using the Stokes-Einstein relationship.

Dynamic light scattering technology from Malvern Instruments offers the following advantages:

  • Accurate, reliable and repeatable particle size analysis in one or two minutes.
  • Measurement in the native environment of the material.
  • Mean size only requires knowledge of the viscosity of the liquid.
  • Simple or no sample preparation, high concentration, turbid samples can be measured directly.
  • Simple set up and fully automated measurement.
  • Size measurement of sizes < 1nm.
  • Size measurement of molecules with MW < 1000Da.
  • Low volume requirement (as little as 2µL).
  • Compliance with regulatory standards ISO 13321, ISO 22412, 21 CFR Part 11.

Related products

Zetasizer range The world's most widely used system for nanoparticle, colloid and protein size, zeta potential and molecular weight measurements. Zetasizer range - particle size, dynamic light scattering, zeta potential, protein aggregation
Molecular size, Particle size, Molecular weight, Zeta potential
Temperature range
Particle size range
0.3nm to 10µm
Dispersion type
Dynamic Light Scattering, Static Light Scattering, Size Exclusion Chromatography, Electrophoretic Light Scattering
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Silver colloids and invisible ink Webinar - Recorded (English)

In the early history of cryptography and ciphers then invisible inks using silver salts and other chemicals were used. Silver colloids were regularly used and abused in this area. Silver colloids are now finding application (and legislation) as bater...

Date recorded:
September 2 2015

Colloidal Stability & Conformational Changes in β-Lactoglobulin: Unfolding Unfolding to Self-Assembly Article (English)

A detailed understanding of the mechanism of unfolding, aggregation, and associated rheological changes is developed in this study for β-Lactoglobulin at different pH values through concomitant measurements utilizing dynamic light scattering (DLS), o...

Combined DLS/Raman provides New Molecular Insights of Complex Fluids Article (English)

In particular, this article demonstrates the correlation between molecular changes (Raman Spectroscopy) and microstructural evolution of rheological properties (DLS, DLS-optical microrheology) for the first time for surfactant-based wormlike micellar...

Non-aqueous zeta potential measurements of carbon black powders Application Note (English)

The measurement of the zeta potential of non-aqueous suspensions is challenging as the electrophoretic mobility of the particles is very small. Here we describe how the Zetasizer Nano in combination with a universal dip cell accessory gives excellent...

Optimizing Small Volume DLS-Microrheology Measurements of Block Co-Polymers Used for Enhanced Oil Recovery Applications Application Note (English)

This note discusses how DLS-based optical microrheology can be used to determine the rheological behavior of polymer solutions and the impact of tracer particle chemistry on the measured microrheological response.

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