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Dynamic light scattering (DLS), sometimes referred to as Quasi-Elastic Light Scattering (QELS), is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1nm.

Typical applications of dynamic light scattering are the characterization of particles, emulsions or molecules, which have been dispersed or dissolved in a liquid. The Brownian motion of particles or molecules in suspension causes laser light to be scattered at different intensities. Analysis of these intensity fluctuations yields the velocity of the Brownian motion and hence the particle size using the Stokes-Einstein relationship.

Dynamic light scattering technology from Malvern Instruments offers the following advantages:

  • Accurate, reliable and repeatable particle size analysis in one or two minutes.
  • Measurement in the native environment of the material.
  • Mean size only requires knowledge of the viscosity of the liquid.
  • Simple or no sample preparation, high concentration, turbid samples can be measured directly.
  • Simple set up and fully automated measurement.
  • Size measurement of sizes < 1nm.
  • Size measurement of molecules with MW < 1000Da.
  • Low volume requirement (as little as 2µL).
  • Compliance with regulatory standards ISO 13321, ISO 22412, 21 CFR Part 11.

Related products

Zetasizer range The world's most widely used system for nanoparticle, colloid and protein size, zeta potential and molecular weight measurements. Zetasizer range - particle size, dynamic light scattering, zeta potential, protein aggregation
Molecular size, Particle size, Molecular weight, Zeta potential
Temperature range
Particle size range
0.3nm to 10µm
Dispersion type
Dynamic Light Scattering, Static Light Scattering, Size Exclusion Chromatography, Electrophoretic Light Scattering
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Characterizing the size, aggregation number and CMC of surfactant micelles using Dynamic Light Scattering Webinar - Live (English)

Surfactant micelles are used in a wide variety of applications from personal care products to pharmaceutical formulations. Dynamic light scattering can be used to characterise micelle size and charge, determine the critical micelle concentration and ...

Zetasizer range
June 23 2016

Combining Dynamic Light Scattering and Raman Spectroscopy for the analysis of protein structure and stability Webinar - Recorded (English)

There are many technologies available for the increased understanding of protein unfolding and stability, including calorimetry, spectroscopy, and light scattering techniques. In recent years, Raman spectroscopy has proven to be useful for the charac...

Date recorded:
March 15 2016

Demo at your desk: Zetasizer Webinar - Recorded (English)

This online demo is designed to provide you with a virtual hands-on introduction to the Zetasizer Nano. You will be able to take a look at the Zetasizer Nano and some of its accessories via webcam. We will screen-share the software with you so that y...

Zetasizer Nano range
Date recorded:
February 25 2016

Suspension stability: Why particle size, zeta potential and rheology are important Webinar - Recorded (English)

Suspensions or dispersions of particles or droplets in a liquid medium are encountered in a variety of industries and find use in a diverse range of applications. These include liquid abrasives, ceramics, medicines, foodstuffs and inks to name a few....

Mastersizer range,Kinexus range,Zetasizer range
Date recorded:
February 11 2016

Size: What is the z-average? FAQ

The z-average diameter is the mean intensity diameter i.e. it is a diameter based upon the intensity of scattered light of a material in its native state (including electrical double layers and surface structure). The z-average is derived from a Cumulants analysis of the measured correlation curve, wherein a single particle size is assumed and a single exponential fit is applied to the autocorrelation function. It is unique to dynamic light scattering and it is sensitive to the presence of aggregates/dust due to the intensity of light scattered by any larger particles present.

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Characterizing the size, aggregation number and CMC of surfactant micelles using Dynamic Light Scattering
(June 23 2016)

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