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Dynamic light scattering (DLS), sometimes referred to as Quasi-Elastic Light Scattering (QELS), is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1nm.

Typical applications of dynamic light scattering are the characterization of particles, emulsions or molecules, which have been dispersed or dissolved in a liquid. The Brownian motion of particles or molecules in suspension causes laser light to be scattered at different intensities. Analysis of these intensity fluctuations yields the velocity of the Brownian motion and hence the particle size using the Stokes-Einstein relationship.

Dynamic light scattering technology from Malvern Instruments offers the following advantages:

  • Accurate, reliable and repeatable particle size analysis in one or two minutes.
  • Measurement in the native environment of the material.
  • Mean size only requires knowledge of the viscosity of the liquid.
  • Simple or no sample preparation, high concentration, turbid samples can be measured directly.
  • Simple set up and fully automated measurement.
  • Size measurement of sizes < 1nm.
  • Size measurement of molecules with MW < 1000Da.
  • Low volume requirement (as little as 2µL).
  • Compliance with regulatory standards ISO 13321, ISO 22412, 21 CFR Part 11.

Related products

Zetasizer range The world's most widely used system for nanoparticle, colloid and protein size, zeta potential and molecular weight measurements. Image
Molecular size, Particle size, Molecular weight, Zeta potential
Temperature range
Particle size range
0.3nm to 10µm
Dispersion type
Dynamic Light Scattering, Static Light Scattering, Size Exclusion Chromatography, Electrophoretic Light Scattering
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Optimizing Small Volume DLS-Microrheology Measurements of Block Co-Polymers Used for Enhanced Oil Recovery Applications Application Note (English)

This note discusses how DLS-based optical microrheology can be used to determine the rheological behavior of polymer solutions and the impact of tracer particle chemistry on the measured microrheological response.

Keywords: Polymer solutions English Application Note Dynamic Light Scattering Oil and petrochemicals Zetasizer Nano ZS Zetasizer Nano ZSP Microrheology 

Suspension properties Masterclass 4: Using rheology to assess suspension stability Webinar - Recorded (English)

This presentation discusses the importance of particle size, zeta potential and rheology for a functional suspension, and demonstrates how these properties can be manipulated to induce dispersion stability.

Kinexus range,Zetasizer Nano range,Mastersizer range
Date recorded:
April 10 2013

Keywords: Suspensions, slurries and pastes English Kinexus range Mastersizer range Particle size Rheology and viscosity Zeta potential Dynamic Light Scattering Electrophoretic Light Scattering Webinar - Recorded Greenwich Time Zetasizer Nano range 

Surface zeta potential of soft, high value and fragile surfaces Poster (English)

We demonstrate the surface zeta potential measurement of pig skin, a human skin analogue used in medicine, pharmaceutical and cosmetic development and cellulose filter paper, used primarily for sterile filtration

Keywords: Surfaces and interfaces English Poster Particle size Zeta potential Dynamic Light Scattering Electrophoretic Light Scattering Cosmetics Zetasizer Nano ZS Zetasizer Nano ZSP Pharmaceutical development 

Experimental progress in high conductivity electrophoresis using the Diffusion Barrier Technique Poster (English)

In this work we demonstrate latex measurements up to 4M KCL using the Diffusion Barrier Technique, thereby demonstrating sample-limited, rather than instrument-limited characterization of high conductivity samples.

Keywords: Nanoparticles English Poster Particle size Zeta potential Dynamic Light Scattering Electrophoretic Light Scattering Colloids Zetasizer Nano ZS Proteins Zetasizer Nano ZSP Protein mobility 
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