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Dynamic light scattering (DLS), sometimes referred to as Quasi-Elastic Light Scattering (QELS), is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1nm.

Typical applications of dynamic light scattering are the characterization of particles, emulsions or molecules, which have been dispersed or dissolved in a liquid. The Brownian motion of particles or molecules in suspension causes laser light to be scattered at different intensities. Analysis of these intensity fluctuations yields the velocity of the Brownian motion and hence the particle size using the Stokes-Einstein relationship.

Dynamic light scattering technology from Malvern Instruments offers the following advantages:

  • Accurate, reliable and repeatable particle size analysis in one or two minutes.
  • Measurement in the native environment of the material.
  • Mean size only requires knowledge of the viscosity of the liquid.
  • Simple or no sample preparation, high concentration, turbid samples can be measured directly.
  • Simple set up and fully automated measurement.
  • Size measurement of sizes < 1nm.
  • Size measurement of molecules with MW < 1000Da.
  • Low volume requirement (as little as 2µL).
  • Compliance with regulatory standards ISO 13321, ISO 22412, 21 CFR Part 11.

Related products

Zetasizer range The worlds most widely used system for nanoparticle, colloid and protein size, zeta potential and molecular weight measurements. Image
Molecular size, Particle size, Molecular weight, Zeta potential
Temperature range
Particle size range
0.3nm to 10µm
Dispersion type
Dynamic Light Scattering, Static Light Scattering, Size Exclusion Chromatography, Electrophoretic Light Scattering
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Colloidal Gold: The Gold Standard for Drug Delivery? Article (English)

This article explains how dynamic light scattering (DLS) can be used to gather particle size distribution data for the successful use of nanosized colloidal gold in drug delivery applications.

Keywords: Nanoparticles English Article Particle size Dynamic Light Scattering Zetasizer Nano range Biological drug characterization and development Injectables Nanotoxicology 

Particle Size Masterclass 2: which technique is best for me? Webinar - Recorded (English)

In the second webinar in the particle size masterclass series, we follow on from why you need to measure particle size, covered in the first masterclass, by looking at how to measure particle size. We started with considerations for choosing a partic...

Date recorded:
March 19 2015

Keywords: English Particle size Dynamic Light Scattering Image analysis Laser diffraction Webinar - Recorded Eastern Time Nanoparticle Tracking Analysis 

A fantastic voyage through the arena of nanoparticle drug delivery Webinar - Recorded (English)

The use of nanoparticles as drug delivery vehicles has seen a large amount of research and investment over the last decade as they have the potential for great benefits in the targeting and efficacy of drug treatments. The interest in them is largely...

Zetasizer range,Archimedes,NanoSight range
Date recorded:
March 18 2015

Keywords: English Zetasizer range Dynamic Light Scattering Webinar - Recorded Eastern Time Resonant mass measurement Archimedes NanoSight range Nanoparticle Tracking Analysis 

Optimised Methodology for Dynamic Light Scattering (DLS) Microrheology Poster (English)

This poster presents an optimized methodology for making microrheology measurements on a Zetasizer Nano ZS/ZSP

Keywords: English Poster Dynamic Light Scattering Zetasizer Nano range Microrheology 

Challenges in the measurement of protein zeta potential Poster (English)

Research shows that aggregation of a protein sample that occurs during electrophoretic measurements is more likely to take place at the electrodes. A new measurement method, the Diffusion Barrier Technique, described in this poster protects the prote...

Keywords: English Poster Particle size Dynamic Light Scattering Electrophoretic Light Scattering Zetasizer Nano ZS Protein aggregation Proteins Zetasizer Nano ZSP Protein mobility Biopharmaceutical development 
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