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Electrophoretic Light Scattering (ELS) is a technique used to measure the electrophoretic mobility of particles in dispersion, or molecules in solution. This mobility is often converted to Zeta potential to enable comparison of materials under different experimental conditions.

The fundamental physical principle is that of electrophoresis. A dispersion is introduced into a cell containing two electrodes. An electrical field is applied to the electrodes, and particles or molecules that have a net charge, or more strictly a net zeta potential will migrate towards the oppositely charged electrode with a velocity, known as the mobility, that is related to their zeta potential.

Malvern Instruments offer leading instrumentation for the measurement of electrophoretic mobility. The Zetasizer Nano provides a simple, fast and accurate way to measure zeta potential, and uses a unique disposable capillary cell to ensure that there is no cross contamination between samples. The NanoSight NS500 measures electrostatic potential particle-by-particle to produce number-weighted, not intensity-weighted, data, avoiding any bias towards larger particles.

Related products

Zetasizer range The world's most widely used system for nanoparticle, colloid and protein size, zeta potential and molecular weight measurements. Zetasizer range - particle size, dynamic light scattering, zeta potential, protein aggregation
Measurement
Molecular size, Particle size, Molecular weight, Zeta potential
Temperature range
90°C
Particle size range
0.3nm to 10µm
Dispersion type
Wet
Technology
Dynamic Light Scattering, Static Light Scattering, Size Exclusion Chromatography, Electrophoretic Light Scattering
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NanoSight NS500 Single particle zeta potential platform with integrated fluidics for automation. NanoSight NS500 single particle size distribution, fluorescence, zeta potential measurment
Measurement
Particle size, Particle concentration, Protein aggregation, Zeta potential
Particle size range
10nm to 2000nm
Technology
Nanoparticle Tracking Analysis, Electrophoretic Light Scattering
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How Do I Verify My Zeta Potential Instrument? FAQ

Electrophoretic light scattering (ELS) or zeta potential instruments use first principles in their measurement protocol. They cannot therefore, be calibrated. However, they can be verified that they are operating correctly, by measuring a suitable zeta potential standard.



How Do I Dilute My Samples For Zeta Potential Measurements? FAQ

Samples for zeta potential measurements need to be optically clear and may require dilution. The way in which the dilution is performed is absolutely critical in determining the zeta potential value measured.



What is Electroosmosis? FAQ

Electroosmosis is the movement of a liquid relative to a stationary charged surface under the influence of an applied voltage



What is Electrophoresis? FAQ

Electrophoresis is the movement of charged particles under the influence of an applied electric field



What is the Derived Count Rate? FAQ

The derived count rate is the normalized count rate taking into account the attenuation factor used


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