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Electrophoretic Light Scattering (ELS) is a technique used to measure the electrophoretic mobility of particles in dispersion, or molecules in solution. This mobility is often converted to Zeta potential to enable comparison of materials under different experimental conditions.

The fundamental physical principle is that of electrophoresis. A dispersion is introduced into a cell containing two electrodes. An electrical field is applied to the electrodes, and particles or molecules that have a net charge, or more strictly a net zeta potential will migrate towards the oppositely charged electrode with a velocity, known as the mobility, that is related to their zeta potential.

The velocity is measured by the laser Doppler technique. There are two implementations of this. One to determine a frequency shift, which can give a full zeta potential distribution and a second known as PALS, where the phase shift is measured. PALS is a more sensitive method, but only gives an average zeta potential value.

The Zetasizer Nano uses both techniques to give the best of both worlds.

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Zetasizer range The worlds most widely used system for nanoparticle, colloid and protein size, zeta potential and molecular weight measurements. Image
Measurement
Molecular size, Particle size, Molecular weight, Zeta potential
Temperature range
Particle size range
Dispersion type
Wet
Technology
Dynamic Light Scattering, Static Light Scattering, Size Exclusion Chromatography, Electrophoretic Light Scattering
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DLS Microrheology on the Zetasizer Nano ZSP/ZS: Use of a zeta potential pre-measurement step to assess tracer particle-sample interactions Application Note (English)

This technical note describes the practical aspects to consider when making a microrheology measurement by dynamic light scattering, and how zeta potential can be used as an indicator of the interaction between tracer particle and sample.


Keywords: English Application Note Zeta potential Electrophoretic Light Scattering Zetasizer Nano ZS Proteins Zetasizer Nano ZSP Microrheology 

Hit the Target Article (English)

Liposomes are fast becoming a preferred vehicle for delivering therapeutic drug molecules to target sites. This article discusses two techniques for the measurement of their size, concentration and zeta potential.


Keywords: Nanoparticles English Article Particle concentration Particle size Zeta potential Dynamic Light Scattering Electrophoretic Light Scattering Liposomes and micelles Zetasizer Nano range NanoSight range Nanoparticle Tracking Analysis 



Improved understanding of the thermal stability of a monoclonal antibody and its modified version by use of DLS/Raman spectroscopy Whitepaper (English)

Use of DLS combined with Raman spectroscopy simultaneously monitors both the colloidal and conformational stability of a template monoclonal antibody (mAb) and a modified monoclonal antibody (mmAb) and provides significant insights into the mechanist...


Keywords: English Whitepaper Molecular structure Molecular size Dynamic Light Scattering Electrophoretic Light Scattering Raman spectroscopy Static Light Scattering Protein aggregation Proteins Biological drug characterization and development Bioformulation development Zetasizer Helix 
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