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Electrophoretic Light Scattering (ELS) is a technique used to measure the electrophoretic mobility of particles in dispersion, or molecules in solution. This mobility is often converted to Zeta potential to enable comparison of materials under different experimental conditions.
The fundamental physical principle is that of electrophoresis. A dispersion is introduced into a cell containing two electrodes. An electrical field is applied to the electrodes, and particles or molecules that have a net charge, or more strictly a net zeta potential will migrate towards the oppositely charged electrode with a velocity, known as the mobility, that is related to their zeta potential.
The velocity is measured by the laser Doppler technique. There are two implementations of this. One to determine a frequency shift, which can give a full zeta potential distribution and a second known as PALS, where the phase shift is measured. PALS is a more sensitive method, but only gives an average zeta potential value.
The Zetasizer Nano uses both techniques to give the best of both worlds.
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Use of the Zetasizer Nano ZSP to assess the structural stability of carbonic anhydrase in the presence and absence of zinc ions by measuring hydrodynamic radius and electrophoretic mobility.
This application note compares the isoelectric point determined using the diffusion barrier technique against that obtained from other charge-measuring techniques. The data shown here shows very good agreement.
Food Science researchers from the University of Massachusetts investigate the efficacy of different production techniques, and the subsequent influence of environmental stresses such as pH, ionic strength and temperature, on emulsion stability.
The Folded Capillary cell (DTS1070) is a maintenance-free, disposable capillary cell for size and zeta potential measurements in the Zetasizer Nano series of instruments. It is a replacement for DTS1060.
An introduction to the principles of froth flotation and the importance of the measurement of zeta potential
Sample measurements performed to demonstrate Zetasizer performance
Introduction to the calculators in the Zetasizer software
Zetasizer Nano Z/ZS/ZS90 Specifications Zeta Potential Maximum Concentration Limit 40% w/v
DTS1070 folded capillary cell - (replacement for DTS1060/DTS1061)
Zetasizer Cells and Cuvettes: Descriptions and Uses
(Webinar - Recorded)
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