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Electrophoretic Light Scattering (ELS) is a technique used to measure the electrophoretic mobility of particles in dispersion, or molecules in solution. This mobility is often converted to Zeta potential to enable comparison of materials under different experimental conditions.
The fundamental physical principle is that of electrophoresis. A dispersion is introduced into a cell containing two electrodes. An electrical field is applied to the electrodes, and particles or molecules that have a net charge, or more strictly a net zeta potential will migrate towards the oppositely charged electrode with a velocity, known as the mobility, that is related to their zeta potential.
The velocity is measured by the laser Doppler technique. There are two implementations of this. One to determine a frequency shift, which can give a full zeta potential distribution and a second known as PALS, where the phase shift is measured. PALS is a more sensitive method, but only gives an average zeta potential value.
The Zetasizer Nano uses both techniques to give the best of both worlds.
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This technical note describes the practical aspects to consider when making a microrheology measurement by dynamic light scattering, and how zeta potential can be used as an indicator of the interaction between tracer particle and sample.
Liposomes are fast becoming a preferred vehicle for delivering therapeutic drug molecules to target sites. This article discusses two techniques for the measurement of their size, concentration and zeta potential.
Overview of the simultaneous and complementary use of DLS combined with Raman spectroscopy to characterize protein size and structural changes and stability
Use of combined DLS and Raman spectroscopy to investigate the evolution of aggregation and the unfolding process of BSA under thermal stress.
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