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Gel Permeation Chromatography (GPC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.
When GPC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size and intrinsic viscosity, and generate information on macromolecular structure, conformation, aggregation and branching.
By using GPC to measure molecular weight and these other properties, scientists can characterize molecules such as synthetic polymers, as well as natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:
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State-of-the-art GPC/SEC systems, such as OMNISEC, offer the sensitivity required to precisely evaluate different grades of polymer-based food additive and to make an optimal choice in formulation
Using a light scattering detector as part of a multi-detector Size Exclusion Chromatography system provides the accurate molecular weight (MW) data needed to assess the activity, safety and clinical efficacy of therapeutic proteins.
The second virial coefficient of Human Serum Albumin (HSA) was measured using a SEC-MALS 20 detector connected to a Refractive Index concentration detector and an online differential viscometer. Two different buffer conditions, Phosphate buffer pH 7....
This poster shows how Rheometry and GPC can be complementary tools for characterizing the behaviour of polysaccharides in solution
This poster describes the use of flow injection polymer analysis (FIPA) as an analytical tool for xylene soluble (XS) determination with quick and easy simultaneous measurements of molecular weight (MW), intrinsic viscosity (IV) and XS%. The techniqu...
(Webinar - Recorded)
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