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Gel Permeation Chromatography (GPC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

When GPC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size and intrinsic viscosity, and generate information on macromolecular structure, conformation, aggregation and branching.

By using GPC to measure molecular weight and these other properties, scientists can characterize molecules such as synthetic polymers, as well as natural polymers such as polysaccharides.

For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:

  • Polymer chemists can control polymer strength, toughness and performance.
  • Pharmaceutical producers can tailor the behavior of products such as eye-drops.
  • Food producers can better understand product performance and quality.

Related products

OMNISEC The world’s most advanced multi-detector GPC/SEC system Image
Measurement
Molecular weight, Molecular size, Molecular structure
Temperature range
20°C to 65°C
Technology
Size Exclusion Chromatography, Static Light Scattering, Gel Permeation Chromatography
More information
Viscotek range The leading multi-detector GPC/SEC systems and detectors. Image
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Temperature range
25°C to 160°C
Technology
Dilute Solution Viscometry, Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
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Zetasizer µV Modular size and molecular weight detector that can be added to any SEC system. Image
Measurement
Molecular size
Temperature range
2°C to 90°C
Particle size range
0.3nm to 2µm
Dispersion type
Wet
Technology
Size Exclusion Chromatography, Dynamic Light Scattering
More information
 
 

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Optimising food additive choice: Exploring the value of recent advances in SEC technology Article (English)

State-of-the-art GPC/SEC systems, such as OMNISEC, offer the sensitivity required to precisely evaluate different grades of polymer-based food additive and to make an optimal choice in formulation


Keywords: English Article Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Food additives Polysaccharides OMNISEC 

The Benefits of Multi-Detector SEC for Protein Analysis Article (English)

Using a light scattering detector as part of a multi-detector Size Exclusion Chromatography system provides the accurate molecular weight (MW) data needed to assess the activity, safety and clinical efficacy of therapeutic proteins.


Keywords: English Article Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Protein aggregation Proteins Biopharmaceutical development Viscotek TDAmax Viscotek TDA 305 

Measuring the 2nd Virial Coeffient and Molecular Density of Proteins to Improve Crystallization Poster (English)

The second virial coefficient of Human Serum Albumin (HSA) was measured using a SEC-MALS 20 detector connected to a Refractive Index concentration detector and an online differential viscometer. Two different buffer conditions, Phosphate buffer pH 7....


Keywords: English Poster Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Proteins Biopharmaceutical development Viscotek SEC-MALS Viscotek TDA 305 

Combined Use of Rheometry and GPC/SEC for Characterising Ionic Polysaccharides Poster (English)

This poster shows how Rheometry and GPC can be complementary tools for characterizing the behaviour of polysaccharides in solution


Keywords: Polymer solutions English Poster Viscotek range Molecular weight Rheology and viscosity Gel Permeation Chromatography Rheometry - rotational Polysaccharides 

An investigation into the effects of aging on automotive bumper samples by xylene soluble flow injection polymer analysis (XS-FIPA) Poster (English)

This poster describes the use of flow injection polymer analysis (FIPA) as an analytical tool for xylene soluble (XS) determination with quick and easy simultaneous measurements of molecular weight (MW), intrinsic viscosity (IV) and XS%. The techniqu...


Keywords: Polymer solutions English Poster Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Automotive and aerospace Viscotek TDAmax 
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