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Gel Permeation Chromatography (GPC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

When GPC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size and intrinsic viscosity, and generate information on macromolecular structure, conformation, aggregation and branching.

By using GPC to measure molecular weight and these other properties, scientists can characterize molecules such as synthetic polymers, as well as natural polymers such as polysaccharides.

For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:

  • Polymer chemists can control polymer strength, toughness and performance.
  • Pharmaceutical producers can tailor the behavior of products such as eye-drops.
  • Food producers can better understand product performance and quality.

Related products

OMNISEC The world’s most advanced multi-detector GPC/SEC system Image
Measurement
Molecular weight, Molecular size, Molecular structure
Temperature range
20°C to 65°C
Technology
Size Exclusion Chromatography, Static Light Scattering, Gel Permeation Chromatography
More information
Viscotek range The leading multi-detector GPC/SEC systems and detectors. Image
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Temperature range
25°C to 160°C
Technology
Dilute Solution Viscometry, Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
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Zetasizer µV Modular size and molecular weight detector that can be added to any SEC system. Image
Measurement
Molecular size
Temperature range
2°C to 90°C
Particle size range
0.3nm to 2µm
Dispersion type
Wet
Technology
Size Exclusion Chromatography, Dynamic Light Scattering
More information
 
 

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Anaysis of PLGA Molecular Weight and Structure by the latest Adavanced Multi-Detector GPC Systems Poster (English)

Different samples of commercially available PLGA were analyzed to compare their absolute molecular weight from light scattering to those quoted with the product. Additionally, the Mark-Houwink plots of different examples containing different ratios o...


Keywords: Polymer solutions English Poster Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography OMNISEC 

Characterizing polymers: Techniques for the measurement of molecular weight, molecular size and branching. Whitepaper (English)

A review of the methods available for measuring the key characteristics of polymers focusing on the benefits and value of gel permeation/size exclusion chromatography (GPC/SEC). Much of the paper talks exclusively about polymers, however many of the ...


Keywords: Polymer solutions English Whitepaper Zetasizer range Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers, plastics and rubbers Adhesives and sealants Paints, inks and coatings Food additives Polysaccharides OMNISEC 

Compositional insights for protein mixtures: a case study of a semi-pure β-amylase extract Application Note (English)

In this application note, we describe the analysis of a semi-purified protein mixture containing β-amylase.  Step-by-step, we show how different properties of the proteins elicit a specific response in each detector and how the coupling this data can...


Keywords: English Application Note Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Protein aggregation Proteins Biopharmaceutical development OMNISEC 

Unsurpassed protein SEC using the latest innovation in advanced detection, OMNISEC Webinar - Recorded (English)

During this webinar, we introduces the latest innovations in SEC technology, and discuss how the new OMNISEC system will assist your protein characterization challenges. Focusing on applications, the webinar compares the capabilities of the system wi...

Product:
OMNISEC
Date recorded:
April 22 2015
Language:
English

Keywords: English Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Webinar - Recorded Eastern Time Protein aggregation Proteins Protein mobility OMNISEC Protein stability 

Structural elucidation of dextrans and other polysaccharides using OMNISEC Multi-Detection GPC Application Note (English)

A series of dextran samples ranging in molecular weight from about 1– 650 kDa are characterized using OMNISEC. Structural comparisons to other polysaccharides, gum arabic and pectin are presented


Keywords: Polymer solutions English Application Note Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers from renewable sources Polysaccharides OMNISEC 
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