Skip to left navigation
Skip to page content
Laser diffraction is a widely used particle sizing technique for materials ranging from hundreds of nanometers up to several millimeters in size. The main reasons for its success are:
Laser diffraction measures particle size distributions by measuring the angular variation in intensity of light scattered as a laser beam passes through a dispersed particulate sample. Large particles scatter light at small angles relative to the laser beam and small particles scatter light at large angles, as illustrated below. The angular scattering intensity data is then analyzed to calculate the size of the particles responsible for creating the scattering pattern, using the Mie theory of light scattering. The particle size is reported as a volume equivalent sphere diameter.
Laser diffraction uses Mie theory of light scattering to calculate the particle size distribution, assuming a volume equivalent sphere model.
Mie theory requires knowledge of the optical properties (refractive index and imaginary component) of both the sample being measured, along with the refractive index of the dispersant. Usually the optical properties of the dispersant are relatively easy to find from published data, and many modern instruments will have in-built databases that include common dispersants. For samples where the optical properties are not known, the user can either measure them or estimate them using an iterative approach based upon the goodness of fit between the modeled data and the actual data collected for the sample.
A simplified approach is to use the Fraunhofer approximation, which does not require knowledge of the optical properties of the sample. This can provide accurate results for large particles. However it should be used with caution whenever working with samples which might have particles below 50µm or where the particles are relatively transparent.
Free access to exclusive content including webinars, presentations, application notes, technical notes, whitepapers and more. Enter your search term in the box and use the drop down boxes to filter the results.
The particle size and size distribution of the Active Pharmaceutical Ingredients (APIs) and excipients present in Oral Solid Dose (OSD) products are often critical to defining product performance. This is recognized by ICH Q6A, which recommends that ...
Suspensions or dispersions of particles or droplets in a liquid medium are encountered in a variety of industries and find use in a diverse range of applications. These include liquid abrasives, ceramics, medicines, foodstuffs and inks to name a few....
This live online demo is designed to provide you with an introduction to the Mastersizer 3000. You will be able to take a look at the Mastersizer 3000 and its accessories via the webcam in our lab, and we will share the Mastersizer 3000 software with...
This webinar covers the basics of particle sizing, including what particles are and what properties of particles are important. We look at definitions of particle size and what the results of a particle size measurement might look like. Several case ...
The particle size of the fat droplets present in dairy and other food emulsions is important in defining properties such as flavor release, mouth feel and emulsion stability. Thus, a knowledge of the particle size is critical in defining the function...
(Webinar - Recorded)
Suspension stability: Why particle size, zeta potential and rheology are important
(February 11 2016)
The software really is the shining star of the Mastersizer 3000. It makes it feel radically different from other systems on the market and a really great instrument to use. I especially like the live trends which make it so quick and easy to compare data sets.
NSL Analytical Services
Throughout the research and purchasing process, we assessed at least five to six different systems before selecting the Mastersizer 3000.
Malvern came recommended by colleagues in the industry but it was the features of the Mastersizer 3000 itself that sold us on the instrument. We’ve been running for a year now without any reliability issues and with minimal running costs. Most importantly we produce data that can be trusted.
All information supplied within is correct at time of publication.
Malvern Instruments pursues a policy of continual improvement due to technical development. We therefore reserve the right to deviate from information, descriptions, and specifications in this publication without notice. Malvern Instruments shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance or use of this material.
Malvern and the 'hills' logo, Bohlin, Gemini, Insitec, ISys, Kinexus, Mastersizer, Morphologi, Rosand, SyNIRgi, Viscotek and Zetasizer, are International Trade Marks owned by Malvern Instruments Ltd.