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Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension.


A laser beam is passed through the sample chamber and the particles in suspension in the path of the beam scatter light in such a manner that they can be easily visualized via a long working distance, x20 magnification microscope onto which is mounted a video camera. The camera captures a video file of the particles moving under Brownian motion. The Nanoparticle Tracking Analysis (NTA) software tracks many particles individually and using the Stokes Einstein equation calculates their hydrodynamic diameters. The NanoSight range of instruments provides high resolution particle size, concentration and aggregation measurements while a fluorescence mode provides specific results for labelled particles. The range provides real time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses confirmed by visual validation.

Measurement of Nanoparticles using Nanoparticle Tracking Analysis

The technique enables the simultaneous measurement of multiple characteristics saving on time and sample volumes while minimal sample preparation and instrument consumables reduces running costs on a day to day basis.

ASTM E2834-12(2012) Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA). Applications include:

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Measurement
Particle size, Particle concentration, Protein aggregation
Particle size range
10nm to 2000nm
Technology
Nanoparticle Tracking Analysis
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Applying complementary particle techniques for assessing comparability and biosimilarity of therapeutic products Webinar - Live (English)

Significant advances have been made in analytical technology for the characterization and identification of particles present in pharmaceutical products. With these advances comes a tremendous amount of new data with which to characterize biologics, ...

Product:
Archimedes,Zetasizer range,Viscotek SEC-MALS,Morphologi G3-ID,NanoSight range
Date:
September 20 2016
Language:
English


NanoSight NS500 Zeta Potential Analysis Technical Note (English)

NanoSight NS500 Zeta Potential Analysis Flow Chart for Use with NTA Software Versions 3.1 and 3.2



Monitoring Microfiltration Processes for Water Treatment Application Note (English)

With the increasing demands on water supplies and tighter regulations, much research is being done on water treatment processes. Subjects of study include improving processes to allow treated water to be used in secondary applications, optimizing fil...



Adeno-Associated Viral Titer in minutes, using Nanoparticle Tracking Analysis (NTA) Webinar - Recorded (English)

• Nanoparticle Tracking Analysis (NTA) Characterization of Gold-Labeled Adeno Associated Virus (AAV) and Other Small Viruses • NTA is an analytical tool that measures the size and concentration of virus particles. • NTA can measure virus titer in abo...

Product:
NanoSight range
Date recorded:
June 7 2016
Language:
English


Case study: Using NTA to study nanoparticle diffusion Webinar - Recorded (English)

• Nanoparticle Tracking Analysis (NTA) was used to study the diffusion of silica nanoparticles functionalized with different polymers in aqueous solutions of water-soluble polymers and in dispersions of porcine gastric mucin • It was found that th...

Product:
NanoSight range
Date recorded:
May 24 2016
Language:
English

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Applying complementary particle techniques for assessing comparability and biosimilarity of therapeutic products
(September 20 2016)

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