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Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension.


A laser beam is passed through the sample chamber and the particles in suspension in the path of the beam scatter light in such a manner that they can be easily visualized via a long working distance, x20 magnification microscope onto which is mounted a video camera. The camera captures a video file of the particles moving under Brownian motion. The Nanoparticle Tracking Analysis (NTA) software tracks many particles individually and using the Stokes Einstein equation calculates their hydrodynamic diameters. The NanoSight range of instruments provides high resolution particle size, concentration and aggregation measurements while a fluorescence mode provides specific results for labelled particles. The range provides real time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses confirmed by visual validation.

Measurement of Nanoparticles using Nanoparticle Tracking Analysis

The technique enables the simultaneous measurement of multiple characteristics saving on time and sample volumes while minimal sample preparation and instrument consumables reduces running costs on a day to day basis.

ASTM E2834-12(2012) Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA). Applications include:

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NanoSight range Characterizing Nanoparticles: Visualizing, Sizing and Concentration NanoSight range - measurement of exosomes, nanoparticles and protein aggregation
Measurement
Particle size, Particle concentration, Protein aggregation
Particle size range
10nm to 2000nm
Technology
Nanoparticle Tracking Analysis
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How to purify and characterize extracellular vesicles Webinar - Recorded (English)

What this webinar covers: - Extracellular vesicles explained - A review of common purifying techniques - An introduction into how Nanoparticle Tracking Analysis (NTA) is used for characterization. - Insights into the latest technology dev...

Product:
NanoSight range
Date recorded:
February 2 2016
Language:
English


Characterizing the properties of liposomes and other drug delivery systems Webinar - Live (English)

Are you involved in researching and developing drug delivery systems? Do you want to characterize their size, charge and concentration? If so, this webinar will be of interest to you. We will discuss techniques capable of providing important informat...

Product:
NanoSight range
Date:
February 16 2016
Language:
English


Comparison of Statistical Measures Reported by NTA and DLS Techniques Technical Note (English)

As the Nanoparticle Tracking Analysis (NTA) technique has gained in popularity in recent years, comparison to Dynamic Light Scattering (DLS) results has become more frequent. When comparing results, it is important to understand the differences betwe...



NTA: Exosomes and Microvesicles - General Whitepaper (English)

The need to characterize different properties of nanomaterials continues to grow rapidly. Since the commercialization of the technique in 2004, Nanoparticle tracking Analysis (NTA) has become increasingly prevalent in a wide variety of different rese...



Nanoparticle Tracking Analysis (NTA) Application: Better Understanding Synthetic Nanoparticle Systems Webinar - Recorded (English)

This webinar introduces the Nanoparticle Tracking Analysis (NTA) technique and how it affords useful information in various synthetic nanoparticle systems. We will discuss how NTA was used to measure the effects of certain buffers on the aggregation...

Product:
NanoSight range
Date recorded:
November 17 2015
Language:
English

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Characterizing the properties of liposomes and other drug delivery systems
(February 16 2016)

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