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Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension.
A laser beam is passed through the sample chamber and the particles in suspension in the path of the beam scatter light in such a manner that they can be easily visualized via a long working distance, x20 magnification microscope onto which is mounted a video camera. The camera captures a video file of the particles moving under Brownian motion. The Nanoparticle Tracking Analysis (NTA) software tracks many particles individually and using the Stokes Einstein equation calculates their hydrodynamic diameters. The NanoSight range of instruments provides high resolution particle size, concentration and aggregation measurements while a fluorescence mode provides specific results for labelled particles. The range provides real time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses confirmed by visual validation.
The technique enables the simultaneous measurement of multiple characteristics saving on time and sample volumes while minimal sample preparation and instrument consumables reduces running costs on a day to day basis.
ASTM E2834-12(2012) Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA). Applications include:
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The need to characterize different properties of nanomaterials continues to grow rapidly. Since the commercialization of the technique in 2004, Nanoparticle tracking Analysis (NTA) has become increasingly prevalent in a wide variety of different rese...
Fluorescence is becoming an increasingly considered as a tool within nanoparticle characterisation. The ability to positively identify particular subsets of nanoparticles within a sample is especially important for applications in life science, drug ...
During this webinar, we will introduce the Nanoparticle Tracking Analysis (NTA) technique and discuss how it can provide a range of useful characterization information for protein corona and self-assembled albumin-drug nanoparticles. Reviews of the ...
The movement of particles in a colloidal suspension is the property we use in order to characterize the size distribution (via the Stokes-Einstein equation). We'll look at diffusion including the random Brownian motion that we require for valid appli...
In this webinar we discuss techniques for producing high quality size distribution and concentration measurements for extracellular vesicle preparations with NanoSight. For characterizing the size and concentration of EVs the focus will be on underst...
(Webinar - Recorded)
Optimizing Exosome and Extracellular Vesicle Measurements by NTA
(August 5 2015)
Malvern Instruments’ webinars, live chats and technical support are very useful and continue to help our researchers push the boundaries of materials and biomaterials science.
Dr Krystelle Mafina,
Queen Mary University,
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