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Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension.

A laser beam is passed through the sample chamber and the particles in suspension in the path of the beam scatter light in such a manner that they can be easily visualized via a long working distance, x20 magnification microscope onto which is mounted a video camera. The camera captures a video file of the particles moving under Brownian motion. The Nanoparticle Tracking Analysis (NTA) software tracks many particles individually and using the Stokes Einstein equation calculates their hydrodynamic diameters. The NanoSight range of instruments provides high resolution particle size, concentration and aggregation measurements while a fluorescence mode provides specific results for labelled particles. The range provides real time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses confirmed by visual validation.


The technique enables the simultaneous measurement of multiple characteristics saving on time and sample volumes while minimal sample preparation and instrument consumables reduces running costs on a day to day basis.

ASTM E2834-12(2012) Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA). Applications include:

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NanoSight range Visualize and measure nanoparticle size and concentration Image
Particle size, Particle concentration, Protein aggregation
Particle size range
10nm to 2000nm
Nanoparticle Tracking Analysis
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The spontaneous emulsion formation (louching) effect Webinar - Recorded (English)

High alcohol based aniseed drinks (e.g. Absinthe, Ouzo, Raki, Sambuca) form a milky emulsion when water is added to them - the so-called Ouzo (or louche) effect. Adding alcohol to this emulsion recovers the clear solution. The size distribution is in...

Date recorded:
February 18 2015

Keywords: English Dynamic Light Scattering Laser diffraction Food and drinks Drinks Webinar - Recorded Eastern Time Nanoparticle Tracking Analysis 

Particle Size Masterclass 2: which technique is best for me? Webinar - Live (English)

In the second webinar in the particle size masterclass series, we will follow on from why you need to measure particle size, covered in the first masterclass, by looking at how to measure particle size. We’ll start with considerations for choosing a ...

March 19 2015

Keywords: English Particle size Dynamic Light Scattering Image analysis Laser diffraction Webinar - Live Eastern Time Nanoparticle Tracking Analysis 

A virtual demonstration of Nanoparticle Tracking Analysis Webinar - Live (English)

This virtual demonstration will give you a brief overview of Nanoparticle Tracking Analysis (NTA). Using NS300 hardware and NTA3.0 software, the analysis of a sample will be covered from sample introduction through to data export and interpretation i...

NanoSight range
January 13 2015

Keywords: English Particle concentration Particle size Webinar - Live Eastern Time Protein aggregation NanoSight range Nanoparticle Tracking Analysis 

Design and formulation of nanomedicines including liposomes Whitepaper (English)

In this white paper the specific mechanisms by which nanoparticles are designed and formulated for therapeutic purposes and in which NTA has had a significant part to play is discussed.

Keywords: Nanoparticles English Whitepaper Particle concentration Particle size Liposomes and micelles Biological drug characterization and development NanoSight range Nanoparticle Tracking Analysis 

Studying nanoparticle-based drug delivery and targeting with Nanoparticle Tracking Analysis Whitepaper (English)

This white paper addresses some of the latest work reported in the literature in which Nanoparticle Tracking Analysis (NTA) has been proposed, used and assessed in the study of nanoparticle-based drug delivery and targeting

Keywords: Nanoparticles English Whitepaper Particle concentration Particle size Biological drug characterization and development NanoSight range Nanoparticle Tracking Analysis 
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Particle Size Masterclass 2: which technique is best for me?
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