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Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension.


A laser beam is passed through the sample chamber and the particles in suspension in the path of the beam scatter light in such a manner that they can be easily visualized via a long working distance, x20 magnification microscope onto which is mounted a video camera. The camera captures a video file of the particles moving under Brownian motion. The Nanoparticle Tracking Analysis (NTA) software tracks many particles individually and using the Stokes Einstein equation calculates their hydrodynamic diameters. The NanoSight range of instruments provides high resolution particle size, concentration and aggregation measurements while a fluorescence mode provides specific results for labelled particles. The range provides real time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses confirmed by visual validation.

Measurement of Nanoparticles using Nanoparticle Tracking Analysis

The technique enables the simultaneous measurement of multiple characteristics saving on time and sample volumes while minimal sample preparation and instrument consumables reduces running costs on a day to day basis.

ASTM E2834-12(2012) Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA). Applications include:

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Measurement
Particle size, Particle concentration, Protein aggregation
Particle size range
10nm to 2000nm
Technology
Nanoparticle Tracking Analysis
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Adeno-Associated Viral Titer in minutes, using Nanoparticle Tracking Analysis (NTA) Webinar - Live (English)

• Nanoparticle Tracking Analysis (NTA) Characterization of Gold-Labeled Adeno Associated Virus (AAV) and Other Small Viruses • NTA is an analytical tool that measures the size and concentration of virus particles. • NTA can measure virus titer in abo...

Product:
NanoSight range
Date:
June 7 2016
Language:
English


Case study: Using NTA to study nanoparticle diffusion Webinar - Recorded (English)

• Nanoparticle Tracking Analysis (NTA) was used to study the diffusion of silica nanoparticles functionalized with different polymers in aqueous solutions of water-soluble polymers and in dispersions of porcine gastric mucin • It was found that th...

Product:
NanoSight range
Date recorded:
May 24 2016
Language:
English


Nanobubbles: What are they, what are their uses and how can I measure them? Webinar - Recorded (English)

The area of fine and ultrafine bubble technology has seen great advancements over the last few years. Ultrafine bubble water has become used in a great deal of cleaning processes (salt, oil, dirt) and also finds uses in hydroponics, ozonation of wate...

Product:
NanoSight range
Date recorded:
May 19 2016
Language:
English


Applying Quality by Design to the manufacture of drug product for biopharmaceuticals Webinar - Recorded (English)

QBD is a term that is often spoken with respect to the production of biopharmaceuticals.  However, despite the issuance of guidance documents on this topic, it is still relatively poorly understood.  This discussion seeks to demystify the concept ...

Product:
NanoSight range,Archimedes
Date recorded:
April 19 2016
Language:
English


Applying Nanoparticle Tracking Analysis to the Study of Drug Delivery Vectors Webinar - Recorded (English)

This webinar covers:• Characterizing size and concentration – the importance for delivery vectors • The challenge of relating in vitro characteristics to in vivo characteristics • Basics of Nanoparticle Tracking Analysis (NTA) • How NTA can ...

Product:
NanoSight range
Date recorded:
April 12 2016
Language:
English

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Adeno-Associated Viral Titer in minutes, using Nanoparticle Tracking Analysis (NTA)
(June 7 2016)

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