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Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension.

A laser beam is passed through the sample chamber, the particles in suspension in the path of the beam scatter light in such a manner that they can be easily visualized via a long working distance, x20 magnification microscope onto which is mounted a video camera. The camera captures a video file of the particles moving under Brownian motion. The software tracks many particles individually and using the Stokes Einstein equation calculates their hydrodynamic diameters. The NanoSight range of instruments provides high resolution particle size, concentration and aggregation measurements while a fluorescence mode provides specific results for labelled particles. The range provides real time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses uniquely confirmed by visual validation.


The technique enables the simultaneous measurement of multiple characteristics saving on time and sample volumes while minimal sample preparation and instrument consumables reduces running costs on a day to day basis.

ASTM E2834-12(2012) Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA). Applications include:

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NanoSight range Visualize and measure nanoparticle size and concentration Image
Particle size, Particle concentration, Protein aggregation
Particle size range
10nm to 2000nm
Nanoparticle Tracking Analysis
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The roles of nanoparticles in therapeutic protein aggregation pathways Webinar - Live (English)

Presenters: John F. Carpenter, Ph.D., Center for Pharmaceutical Biotechnology, Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Center Pauline Carnell, Product Manager ...

NanoSight range
September 25 2014

Keywords: Nanoparticles English Particle concentration Particle size Viruses and vaccines Webinar - Live Eastern Time Protein aggregation Proteins Biopharmaceutical development NanoSight range Nanoparticle Tracking Analysis 

Exosomes and Microvesicles: An introduction Whitepaper (English)

The study of exosomes and other cell-derived microvesicles is an area of rapidly growing importance and the subject of intense interest and research. The previous lack of suitable methods for their detection, analysis, enumeration and phenoty...

Keywords: English Whitepaper Particle concentration Particle size NanoSight range Extracellular vesicles Nanoparticle Tracking Analysis 

Characterization of nanobubbles and other ultrafine bubbles by Nanoparticle Tracking Analysis (NTA) Whitepaper (English)

Acceptance of and interest in the special properties of nanobubbles and other ultrafine bubbles is growing rapidly, and their formation and characteristics are the subject of an increasing amount of study, particularly in Europe and Japan...

Keywords: English Whitepaper Particle concentration Particle size NanoSight range Nanobubbles Nanoparticle Tracking Analysis 

Extending the options for particle characterisation Article (English)

Particle size is a core performance defining characteristic throughout the metal powders and ceramics industries. As new materials and production methods are developed, an increasing range of techniques is needed to understand the materia...

Keywords: Nanoparticles English Article Particle concentration Particle size NanoSight range Nanoparticle Tracking Analysis 

Hit the Target Article (English)

Liposomes are fast becoming a preferred vehicle for delivering therapeutic drug molecules to target sites. This article discusses two techniques for the measurement of their size, concentration and zeta potential.

Keywords: Nanoparticles English Article Particle concentration Particle size Zeta potential Dynamic Light Scattering Electrophoretic Light Scattering Liposomes and micelles Zetasizer Nano range NanoSight range Nanoparticle Tracking Analysis 
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The roles of nanoparticles in therapeutic protein aggregation pathways
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