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Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension.

A laser beam is passed through the sample chamber and the particles in suspension in the path of the beam scatter light in such a manner that they can be easily visualized via a long working distance, x20 magnification microscope onto which is mounted a video camera. The camera captures a video file of the particles moving under Brownian motion. The Nanoparticle Tracking Analysis (NTA) software tracks many particles individually and using the Stokes Einstein equation calculates their hydrodynamic diameters. The NanoSight range of instruments provides high resolution particle size, concentration and aggregation measurements while a fluorescence mode provides specific results for labelled particles. The range provides real time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses confirmed by visual validation.

Measurement of Nanoparticles using Nanoparticle Tracking Analysis

The technique enables the simultaneous measurement of multiple characteristics saving on time and sample volumes while minimal sample preparation and instrument consumables reduces running costs on a day to day basis.

ASTM E2834-12(2012) Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA). Applications include:

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NTA: Exosomes and Microvesicles - General Whitepaper (English)

The need to characterize different properties of nanomaterials continues to grow rapidly. Since the commercialization of the technique in 2004, Nanoparticle tracking Analysis (NTA) has become increasingly prevalent in a wide variety of different rese...

Nanoparticle Tracking Analysis (NTA) Application: Better Understanding Synthetic Nanoparticle Systems Webinar - Recorded (English)

This webinar introduces the Nanoparticle Tracking Analysis (NTA) technique and how it affords useful information in various synthetic nanoparticle systems. We will discuss how NTA was used to measure the effects of certain buffers on the aggregation...

NanoSight range
Date recorded:
November 17 2015

NTA Virtual Demonstration Webinar - Recorded (English)

Nanoparticle Tracking Analysis (NTA) has been widely adopted in a range of different research streams for the visualisation, sizing and counting of nanoscale materials in liquid suspension. Here the methodology behind the instrumentation will be disc...

NanoSight range
Date recorded:
September 22 2015

Measuring Zeta Potential Standards with NanoSight NTA Technical Note (English)

Unlike standards, many sample particles have a range of zeta potential values and Z-NTA measurements can provide high resolution particle-by particle zeta potential information. This is especially critical where small changes in the zeta potential di...

Measuring NIST Standards with NanoSight NTA Technical Note (English)

To ensure that your NanoSight instrument is performing within specification, it is best practice to regularly measure size-traceable standards. The size standard/s used should be similar to the size of the nanoparticles which are typically measured w...

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