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Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension.
A laser beam is passed through the sample chamber and the particles in suspension in the path of the beam scatter light in such a manner that they can be easily visualized via a long working distance, x20 magnification microscope onto which is mounted a video camera. The camera captures a video file of the particles moving under Brownian motion. The Nanoparticle Tracking Analysis (NTA) software tracks many particles individually and using the Stokes Einstein equation calculates their hydrodynamic diameters. The NanoSight range of instruments provides high resolution particle size, concentration and aggregation measurements while a fluorescence mode provides specific results for labelled particles. The range provides real time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses confirmed by visual validation.
The technique enables the simultaneous measurement of multiple characteristics saving on time and sample volumes while minimal sample preparation and instrument consumables reduces running costs on a day to day basis.
ASTM E2834-12(2012) Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA). Applications include:
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Significant advances have been made in analytical technology for the characterization and identification of particles present in pharmaceutical products. With these advances comes a tremendous amount of new data with which to characterize biologics, ...
• Nanoparticle Tracking Analysis (NTA) Characterization of Gold-Labeled Adeno Associated Virus (AAV) and Other Small Viruses • NTA is an analytical tool that measures the size and concentration of virus particles. • NTA can measure virus titer in abo...
• Nanoparticle Tracking Analysis (NTA) was used to study the diffusion of silica nanoparticles functionalized with different polymers in aqueous solutions of water-soluble polymers and in dispersions of porcine gastric mucin
• It was found that th...
The area of fine and ultrafine bubble technology has seen great advancements over the last few years. Ultrafine bubble water has become used in a great deal of cleaning processes (salt, oil, dirt) and also finds uses in hydroponics, ozonation of wate...
Aggregation of both biological and synthetic materials is a serious cause for concern, posing problems at various stages of the discovery and formulation processes. Liposomes have been used in drug discovery and drug delivery for some time, and the b...
(Webinar - Recorded)
Applying complementary particle techniques for assessing comparability and biosimilarity of therapeutic products
(September 20 2016)
The Malvern NanoSight NTA system allows us to directly and reliably quantify extracellular vesicle yields from the cell lines under investigation, which is critical to this project.
Ohio State University
With NTA we are able to sensitively monitor the behavior of a nanoparticle over time in a variety of different media.
Département Environnement et Agro-biotechnologies at the CRP - Gabriel Lippmann.
NTA is a relatively new addition to the lab but is already bringing value to several areas of research.
Dr Krystelle Mafina
Queen Mary University
Malvern Instruments’ webinars, live chats and technical support are very useful and continue to help our researchers push the boundaries of materials and biomaterials science.
One of the great things about the Nanoparticle Tracking Analysis system is the unique visualization capability which enables real time empirical insight into key particle behavior, such as the propensity and rate of aggregation, as opposed to alternative techniques with often lengthy procedures.
University of Wisconsin-Milwaukee
Nanoparticle Tracking Analysis has provided us with an instantaneous way of looking at the behavior of the nanoparticles within a biological medium, providing insight unachievable with alternative analysis techniques.
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