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Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension.


A laser beam is passed through the sample chamber and the particles in suspension in the path of the beam scatter light in such a manner that they can be easily visualized via a long working distance, x20 magnification microscope onto which is mounted a video camera. The camera captures a video file of the particles moving under Brownian motion. The Nanoparticle Tracking Analysis (NTA) software tracks many particles individually and using the Stokes Einstein equation calculates their hydrodynamic diameters. The NanoSight range of instruments provides high resolution particle size, concentration and aggregation measurements while a fluorescence mode provides specific results for labelled particles. The range provides real time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses confirmed by visual validation.

Measurement of Nanoparticles using Nanoparticle Tracking Analysis

The technique enables the simultaneous measurement of multiple characteristics saving on time and sample volumes while minimal sample preparation and instrument consumables reduces running costs on a day to day basis.

ASTM E2834-12(2012) Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA). Applications include:

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NanoSight range Characterizing Nanoparticles: Visualizing, Sizing and Concentration Image
Measurement
Particle size, Particle concentration, Protein aggregation
Particle size range
10nm to 2000nm
Technology
Nanoparticle Tracking Analysis
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NTA: Exosomes and Microvesicles - General Whitepaper (English)

The need to characterize different properties of nanomaterials continues to grow rapidly. Since the commercialization of the technique in 2004, Nanoparticle tracking Analysis (NTA) has become increasingly prevalent in a wide variety of different rese...


Keywords: English Whitepaper Particle concentration Particle size NanoSight NS500 NanoSight NS300 NanoSight LM10 Exosomes and microvesicles Nanoparticle Tracking Analysis Fluorescence detection 

NTA Concentration Upgrade Webinar - Recorded (English)

An overview of the NTA Concentration Measurement upgrade which gives improved concentration data by further reducing the influence of user settings and allowing improved inter- and intra-laboratory comparison of concentration data.

Product:
NanoSight range,NanoSight NS300,NanoSight NS500
Date recorded:
June 18 2015
Language:
English

Keywords: Nanoparticles English DNA/RNA and oligonucleotides Liposomes and micelles Viruses and vaccines Webinar - Recorded Greenwich Time NanoSight range NanoSight NS500 NanoSight NS300 Exosomes and microvesicles Nanoparticle Tracking Analysis 

NTA Fluorescence mode Webinar - Recorded (English)

This short presentation will give an overview of fluorescence and what you need to think about before you start making measurements of fluorescent samples with NanoSight instruments.

Product:
NanoSight range
Date recorded:
June 18 2015
Language:
English

Keywords: Nanoparticles English DNA/RNA and oligonucleotides Liposomes and micelles Viruses and vaccines Webinar - Recorded Greenwich Time NanoSight range Exosomes and microvesicles Nanoparticle Tracking Analysis Fluorescence detection 

Fluorescence Masterclass - The basics Webinar - Recorded (English)

Fluorescence is increasingly considered as a tool within nanoparticle characterisation. The ability to positively identify particular subsets of nanoparticles within a sample is especially important for applications in life science, drug delivery, vi...

Product:
NanoSight range
Date recorded:
June 16 2015
Language:
English

Keywords: English Webinar - Recorded Eastern Time NanoSight range Nanoparticle Tracking Analysis Fluorescence detection 

NanoSight NTA Concentration Measurement Upgrade Application Note (English)

The inter- and intra- laboratory repeatability and reproducibility are greatly improved with the application of the NTA concentration measurement upgrade, which has also been shown to reduce the influence of user selected capture and analysis setti...


Keywords: Nanoparticles English Application Note Particle concentration Colloids Liposomes and micelles Viruses and vaccines Sub-visible particles Proteins Biological drug characterization and development Bioformulation development Antibodies and derivatives NanoSight NS500 NanoSight NS300 Nanotoxicology Exosomes and microvesicles Nanobubbles Nanoparticle Tracking Analysis 
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Glossary

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The Malvern NanoSight NTA system allows us to directly and reliably quantify extracellular vesicle yields from the cell lines under investigation, which is critical to this project.


Dr Paulaitis, Ohio State University, United States

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