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Size Exclusion Chromatography (SEC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.
When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.
By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:
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This article gives an introduction to multi-detection GPC/SEC and presents data that shows how the technique is being used to determine the impact of sterilization on medical grade poly(L-lactide).
Cations are specifically recognized by numerous proteins. Despite their small size, their specific interactions with highly charged residues allow them to induce significant conformational changes on their binding proteins. The protein conformational...
In this application note, interferon α-5 was conjugated with two different PEG molecules in different reactions. The individual protein and PEG molecules, as well as the PEGylated conjugates are characterized by SEC and their composition,...
This technical note describes the two different ways of operating the seal wash system in the Viscotek GPCmax and VE-1122 pumps, which are essential for maximising the lifetime of seals and pistons.
A description of how advanced GPC/SEC detection can overcome inaccuracy in the measurement of molecular weight caused by deviation in the structure of the sample protein from that of the protein standards or interaction with the chromatography column...
(Webinar - Recorded)
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