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Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.
When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.
By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:
For polymer and protein characterization, in polymer, food, pharmaceutical and biopharmaceutical research, Malvern offers a range of SEC systems or individual SEC detectors, including SEC-MALS, to suit your application needs.
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Significant advances have been made in analytical technology for the characterization and identification of particles present in pharmaceutical products. With these advances comes a tremendous amount of new data with which to characterize biologics, ...
Gel Permeation Chromatography (GPC) also known as Size Exclusion Chromatography (SEC), is a very popular analytical tool for characterizing the molecular weight and structure of natural and synthetic polymers. Such information is important for both r...
Gel Permeation Chromatography (GPC), is a very popular analytical tool for polymer characterization. Key industries where GPC is used include the food and drink, paper, and personal care industries, among others, where polysaccharides such as cellulo...
Size Exclusion Chromatography (SEC) is a valuable technique for the analysis of macromolecules such as proteins, antibodies, and polysaccharides. Characterization of these materials is essential for many applications, ranging from pharmaceutical/biop...
Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is the gold standard in analysis of natural and synthetic polymers. Including a viscometer in your GPC system enables characterization of the structure of your po...
(Webinar - Recorded)
Applying complementary particle techniques for assessing comparability and biosimilarity of therapeutic products
(September 20 2016)
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