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Size Exclusion Chromatography (SEC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.
When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.
By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:
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This webinar will discuss how Malvern’s protein SEC measurement capabilities within the Viscotek range have developed and improved in the last year. We will also introduce the new features that are included in the latest release of OmniSEC, i...
In this application note, a sample of Whey Protein Isolate (WPI) was characterized using the Viscotek TDAmax. The 4 main components in this mixture were separated and individually characterized to determine molecular weight and size.
In this application note, triple detection size exclusion chromatography (SEC-TD) is used to understand the ability of proteins to form ordered, structured complexes. The controlling mechanism behind these protein complexes is fundamental to ...
This article gives an introduction to multi-detection GPC/SEC and presents data that shows how the technique is being used to determine the impact of sterilization on medical grade poly(L-lactide).
Cations are specifically recognized by numerous proteins. Despite their small size, their specific interactions with highly charged residues allow them to induce significant conformational changes on their binding proteins. The protein conformational...
(Webinar - Recorded)
Powerful protein SEC made simple!
(September 30 2014)
(Webinar - Live)
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