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Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.

By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:

  • Biochemists can understand protein oligomerization, aggregation, conjugation and conformation.
  • Polymer chemists can control polymer strength, toughness and performance.
  • Pharmaceutical producers can tailor the behavior of products such as eye-drops.
  • Food producers can better understand product performance and quality.

For polymer and protein characterization, in polymer, food, pharmaceutical and biopharmaceutical research, Malvern offers a range of SEC systems or individual SEC detectors, including SEC-MALS, to suit your application needs.

Related products

Zetasizer µV Modular size and molecular weight detector that can be added to any SEC system. Image
Measurement
Molecular size
Temperature range
2°C to 90°C
Particle size range
0.3nm to 2µm
Dispersion type
Wet
Technology
Size Exclusion Chromatography, Dynamic Light Scattering
More information
OMNISEC The world’s most advanced multi-detector GPC/SEC system molecular weight, molecular size, molecular structure, intrinsic viscosity by size exclusion chromatography with OMNISEC
Measurement
Molecular weight, Molecular size, Molecular structure
Molecular weight range
200Da to 10MDa
Minimum sample mass (polystyrene)
100ng
Minimum sample mass (BSA)
100ng
Technology
Size Exclusion Chromatography, Static Light Scattering, Gel Permeation Chromatography
More information
Viscotek range The leading multi-detector GPC/SEC systems and detectors. molecular weight, molecular size, molecular structure, intrinsic viscosity by gel permeation chromatography with Viscotek
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Molecular weight range
1000Da to 10MDa
Minimum sample mass (polystyrene)
1µg
Minimum sample mass (BSA)
1µg
Technology
Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
More information
 
 

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Fast, easy and reliable – Flow Injection Polymer Analysis for QA/QC and Process Control Webinar - Recorded (English)

Gel Permeation Chromatography (GPC) also known as Size Exclusion Chromatography (SEC), is a very popular analytical tool for characterizing the molecular weight and structure of natural and synthetic polymers. Such information is important for both r...

Product:
OMNISEC
Date recorded:
April 14 2016
Language:
English


Characterizing Polymeric Gelling Agents using Gel Permeation Chromatography. Are You Gelling? Webinar - Recorded (English)

Gel Permeation Chromatography (GPC), is a very popular analytical tool for polymer characterization. Key industries where GPC is used include the food and drink, paper, and personal care industries, among others, where polysaccharides such as cellulo...

Product:
OMNISEC
Date recorded:
March 10 2016
Language:
English


How Size Exclusion Chromatography can improve processes and quality in biopharmaceutical development Webinar - Recorded (English)

Size Exclusion Chromatography (SEC) is a valuable technique for the analysis of macromolecules such as proteins, antibodies, and polysaccharides. Characterization of these materials is essential for many applications, ranging from pharmaceutical/biop...

Product:
OMNISEC
Date recorded:
March 1 2016
Language:
English


Improving polymer performance: How adding a viscometer to your GPC system can help Webinar - Recorded (English)

Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is the gold standard in analysis of natural and synthetic polymers. Including a viscometer in your GPC system enables characterization of the structure of your po...

Product:
OMNISEC
Date recorded:
January 26 2016
Language:
English


History and development of Malvern GPC Viscometers Webinar - Recorded (English)

Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is a very popular analytical toll for characterizing natural and synthetic polymers. The introduction of the first differential Viscometer (Viscotek Model H100) b...

Product:
Viscotek range,OMNISEC
Date recorded:
November 10 2015
Language:
English

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