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Size Exclusion Chromatography (SEC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.

By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:

  • Biochemists can understand protein oligomerization, aggregation, conjugation and conformation.
  • Polymer chemists can control polymer strength, toughness and performance.
  • Pharmaceutical producers can tailor the behavior of products such as eye-drops.
  • Food producers can better understand product performance and quality.

Related products

Zetasizer µV Modular size and molecular weight detector that can be added to any SEC system. Image
Measurement
Molecular size
Temperature range
2°C to 90°C
Particle size range
0.3nm to 2µm
Dispersion type
Wet
Technology
Size Exclusion Chromatography, Dynamic Light Scattering
More information
OMNISEC The world’s most advanced multi-detector GPC/SEC system Image
Measurement
Molecular weight, Molecular size, Molecular structure
Temperature range
20°C to 65°C
Technology
Size Exclusion Chromatography, Static Light Scattering, Gel Permeation Chromatography
More information
Viscotek range The leading multi-detector GPC/SEC systems and detectors. Image
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Temperature range
25°C to 160°C
Technology
Dilute Solution Viscometry, Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
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OMNISEC for unsurpassed synthetic polymer characterization Webinar - Recorded (English)

Realize the maximum potential of your polymer using unsurpassed characterization of molecular weight and structure using OMNISEC – our latest gel permeation chromatography / size exclusion chromatography system. Dr. Mark Pothecary, Product Manager, ...

Date recorded:
February 17 2015
Language:
English

Keywords: English Molecular size Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Polymers, plastics and rubbers Webinar - Recorded Eastern Time 

Accelerating the Development of Generic Pharmaceuticals: Developing an Analytical Toolkit Suitable for De-formulating Complex Reference Products Article (English)

In its report ‘Critical Path Opportunities for Generic Drugs’, the FDA emphasizes the need for advances in the field of analytical sciences in order to accelerate the development of generic products. Here, Paul Kippax highlights techniques that are e...


Keywords: Development English Article Chemical identification Molecular weight Particle shape Particle size Gel Permeation Chromatography Image analysis Raman spectroscopy Size Exclusion Chromatography Pharmaceutical formulation and development 

Understanding the effect of molecular weight and structure on the performance characteristics of cellulose derivatives using OMNISEC Application Note (English)

A variety of aqueous (water-soluble) cellulose derivatives were characterized using the OMNISEC triple detection GPC/SEC system which revealed subtle, yet distinct differences between them. The differences described in this application note could be ...


Keywords: Polymer solutions English Application Note Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers from renewable sources Pharmaceutical development Excipients and coatings OMNISEC 

Characterization of IgG monomers & their aggregates Application Note (English)

Two antibody samples were characterized using column calibration and multi-detection SEC, and the results compared to show how multi-detection SEC provides more accurate data and a more complete characterization of the protein mixtures under study.


Keywords: English Application Note Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Protein aggregation Proteins Biopharmaceutical development Antibodies and derivatives OMNISEC 

GPC/SEC analysis of polylactic acid (PLA) and poly(lactic-co-glycolic acid) (PLGA) using OMNISEC Application Note (English)

In this application note, a selection of different PLA and PLGA polymer samples were analyzed on Malvern's OMNISEC GPC/SEC system, which combines multiple detectors to provide information about structure and solution properties.


Keywords: Polymer solutions English Application Note Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Polymers from renewable sources Specialty and novel polymers Polymer recycling OMNISEC 
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