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Size Exclusion Chromatography (SEC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.
When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.
By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:
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Realize the maximum potential of your polymer using unsurpassed characterization of molecular weight and structure using OMNISEC – our latest gel permeation chromatography / size exclusion chromatography system.
Dr. Mark Pothecary, Product Manager, ...
In its report ‘Critical Path Opportunities for Generic Drugs’, the FDA emphasizes the need for advances in the field of analytical sciences in order to accelerate the development of generic products. Here, Paul Kippax highlights techniques that are e...
A variety of aqueous (water-soluble) cellulose derivatives were characterized using the OMNISEC triple detection GPC/SEC system which revealed subtle, yet distinct differences between them. The differences described in this application note could be ...
Two antibody samples were characterized using column calibration and multi-detection SEC, and the results compared to show how multi-detection SEC provides more accurate data and a more complete characterization of the protein mixtures under study.
In this application note, a selection of different PLA and PLGA polymer samples were analyzed on Malvern's OMNISEC GPC/SEC system, which combines multiple detectors to provide information about structure and solution properties.
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