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Size Exclusion Chromatography (SEC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.

By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:

  • Biochemists can understand protein oligomerization, aggregation, conjugation and conformation.
  • Polymer chemists can control polymer strength, toughness and performance.
  • Pharmaceutical producers can tailor the behavior of products such as eye-drops.
  • Food producers can better understand product performance and quality.

Related products

Zetasizer µV Modular size and molecular weight detector that can be added to any SEC system. Image
Measurement
Molecular size
Temperature range
2°C to 90°C
Particle size range
0.0003µm to 2µm
Dispersion type
Wet
Technology
Size Exclusion Chromatography, Dynamic Light Scattering
More information
Viscotek range The leading multi-detector GPC/SEC systems and detectors. Image
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Accuracy
Technology
Dilute Solution Viscometry, Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
More information
 
 

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Exploring the role of SEC-MALS in protein and polymer characterization Webinar - Recorded (English)

Size exclusion chromatography (SEC) is a chromatographic technique for the characterization of proteins and biopharmaceuticals, as well as natural and synthetic polymers. Traditional systems comprised a single concentration detector (Conventional Cal...

Product:
Viscotek SEC-MALS,Viscotek 270max,Viscotek TDAmax,Viscotek RImax
Date recorded:
October 17 2014
Language:
English

Keywords: Polymer solutions English Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Food additives Rubbers and elastomers Specialty and novel polymers Membrane proteins Polysaccharides Webinar - Recorded GMT Time Protein aggregation Proteins Bioformulation development Antibodies and derivatives Viscotek 270max Viscotek RImax Viscotek TDAmax Viscotek SEC-MALS 

Powerful protein SEC made simple! Webinar - Recorded (English)

This webinar discusses how Malvern’s protein SEC measurement capabilities within the Viscotek range have developed and improved in the last year. We also introduce the new features that are included in the latest release of OmniSEC, including: Bio...

Product:
Viscotek range,Viscotek RImax,Viscotek DSV,Viscotek 270max,Viscotek TDAmax,Viscotek SEC-MALS
Date recorded:
September 30 2014
Language:
English

Keywords: English Viscotek range Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Webinar - Recorded Eastern Time Protein aggregation Proteins Biopharmaceutical development Viscotek 270max Viscotek DSV Viscotek RImax Viscotek TDAmax Viscotek SEC-MALS 

Characterization of Whey Protein Isolate (WPI) using multi-detector size-exclusion chromatography Application Note (English)

In this application note, a sample of Whey Protein Isolate (WPI) was characterized using the Viscotek TDAmax. The 4 main components in this mixture were separated and individually characterized to determine molecular weight and size. ...


Keywords: English Application Note Molecular size Molecular weight Size Exclusion Chromatography Dairy products Food additives Protein aggregation Proteins Viscotek TDAmax Viscotek TDA 305 

Characterization of Protein-Protein Interactions using triple detection size exclusion chromatography (SEC-TD) Application Note (English)

In this application note, triple detection size exclusion chromatography (SEC-TD) is used to understand the ability of proteins to form ordered, structured complexes. The controlling mechanism behind these protein complexes is fundamental to ...


Keywords: English Application Note Molecular size Molecular weight Static Light Scattering Size Exclusion Chromatography Viruses and vaccines Proteins Biological drug characterization and development Viscotek TDAmax Viscotek TDA 305 

Using Multi-Detection GPC/SEC to Determine Impact of Sterilization on Medical-Grade Polymers Article (English)

This article gives an introduction to multi-detection GPC/SEC and presents data that shows how the technique is being used to determine the impact of sterilization on medical grade poly(L-lactide).


Keywords: Polymer solutions English Article Molecular structure Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Specialty and novel polymers Injectables Viscotek TDAmax 
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