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Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.

By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:

  • Biochemists can understand protein oligomerization, aggregation, conjugation and conformation.
  • Polymer chemists can control polymer strength, toughness and performance.
  • Pharmaceutical producers can tailor the behavior of products such as eye-drops.
  • Food producers can better understand product performance and quality.

For polymer and protein characterization, in polymer, food, pharmaceutical and biopharmaceutical research, Malvern offers a range of SEC systems or individual SEC detectors, including SEC-MALS, to suit your application needs.

Related products

Zetasizer µV Modular size and molecular weight detector that can be added to any SEC system. Image
Measurement
Molecular size
Temperature range
2°C to 90°C
Particle size range
0.3nm to 2µm
Dispersion type
Wet
Technology
Size Exclusion Chromatography, Dynamic Light Scattering
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OMNISEC The world’s most advanced multi-detector GPC/SEC system molecular weight, molecular size, molecular structure, intrinsic viscosity by size exclusion chromatography with OMNISEC
Measurement
Molecular weight, Molecular size, Molecular structure
Molecular weight range
200Da to 10MDa
Minimum sample mass (polystyrene)
100ng
Minimum sample mass (BSA)
100ng
Technology
Size Exclusion Chromatography, Static Light Scattering, Gel Permeation Chromatography
More information
Viscotek range The leading multi-detector GPC/SEC systems and detectors. molecular weight, molecular size, molecular structure, intrinsic viscosity by gel permeation chromatography with Viscotek
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Molecular weight range
1000Da to 10MDa
Minimum sample mass (polystyrene)
1µg
Minimum sample mass (BSA)
1µg
Technology
Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
More information
 
 

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Applying complementary particle techniques for assessing comparability and biosimilarity of therapeutic products Webinar - Live (English)

Significant advances have been made in analytical technology for the characterization and identification of particles present in pharmaceutical products. With these advances comes a tremendous amount of new data with which to characterize biologics, ...

Product:
Archimedes,Zetasizer range,Viscotek SEC-MALS,Morphologi G3-ID,NanoSight range
Date:
September 20 2016
Language:
English


OMNISEC: Demo at your desk Webinar - Live (English)

Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is a very popular analytical technique for characterizing natural and synthetic polymers. Malvern offers a range of multi-detector GPC systems for the advanced ch...

Product:
OMNISEC
Date:
September 29 2016
Language:
English


How multi-detector GPC/SEC can advance our understanding of drug delivery polymers Webinar - Live (English)

Correctly targeting a drug product’s delivery location and defining its release profile are critical challenges and the subject of a great deal of research. The role of polymers in drug delivery applications is growing, with particular emphasis on t...

Product:
Viscotek range,OMNISEC,Zetasizer range
Date:
September 6 2016
Language:
English


Fast, easy and reliable – Flow Injection Polymer Analysis for QA/QC and Process Control Webinar - Recorded (English)

Gel Permeation Chromatography (GPC) also known as Size Exclusion Chromatography (SEC), is a very popular analytical tool for characterizing the molecular weight and structure of natural and synthetic polymers. Such information is important for both r...

Product:
OMNISEC
Date recorded:
April 14 2016
Language:
English


Characterizing Polymeric Gelling Agents using Gel Permeation Chromatography. Are You Gelling? Webinar - Recorded (English)

Gel Permeation Chromatography (GPC), is a very popular analytical tool for polymer characterization. Key industries where GPC is used include the food and drink, paper, and personal care industries, among others, where polysaccharides such as cellulo...

Product:
OMNISEC
Date recorded:
March 10 2016
Language:
English

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Upcoming webinar

How multi-detector GPC/SEC can advance our understanding of drug delivery polymers
(September 6 2016)

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