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Size Exclusion Chromatography (SEC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.

By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:

  • Biochemists can understand protein oligomerization, aggregation, conjugation and conformation.
  • Polymer chemists can control polymer strength, toughness and performance.
  • Pharmaceutical producers can tailor the behavior of products such as eye-drops.
  • Food producers can better understand product performance and quality.

Related products

Zetasizer µV Modular size and molecular weight detector that can be added to any SEC system. Image
Measurement
Molecular size
Temperature range
2°C to 90°C
Particle size range
0.3nm to 2µm
Dispersion type
Wet
Technology
Size Exclusion Chromatography, Dynamic Light Scattering
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OMNISEC The world’s most advanced multi-detector GPC/SEC system molecular weight, molecular size, molecular structure, intrinsic viscosity by size exclusion chromatography with OMNISEC
Measurement
Molecular weight, Molecular size, Molecular structure
Molecular weight range
200Da to 10MDa
Minimum sample mass (polystyrene)
100ng
Minimum sample mass (BSA)
100ng
Technology
Size Exclusion Chromatography, Static Light Scattering, Gel Permeation Chromatography
More information
Viscotek range The leading multi-detector GPC/SEC systems and detectors. molecular weight, molecular size, molecular structure, intrinsic viscosity by gel permeation chromatography with Viscotek
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Molecular weight range
1000Da to 10MDa
Minimum sample mass (polystyrene)
1µg
Minimum sample mass (BSA)
1µg
Technology
Dilute Solution Viscometry, Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
More information
 
 

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How low can you go? New advances in the analysis of low molecular weight polymers by GPC/SEC Webinar - Recorded (English)

Gel Permeation Chromatography (GPC) also known as Size Exclusion Chromatography (SEC), is a very popular analytical tool for polymer characterization. Low molecular weight polymers such as polyols, epoxies and polycaprolactones (amongst others) are u...

Product:
OMNISEC
Date recorded:
August 25 2015
Language:
English


From 0 to 60 – Novel polymer applications in the automotive industry Webinar - Live (English)

There is a strong global trend in automotive design that features a continuous increase in the use of conventional, performance, and novel polymeric materials. This is a result of weight savings, design flexibility, and ease of fabrication offered by...

Date:
September 3 2015
Language:
English


Characterization of IgG monomers & their aggregates Application Note (English)

Two antibody samples were characterized using column calibration and multi-detection SEC, and the results compared to show how multi-detection SEC provides more accurate data and a more complete characterization of the protein mixtures under study.



The Mark–Houwink plot – Differentiation of Branching and Composition Application Note (English)

Using OMNISEC, we will show how to separate the effects of a structural difference induced by a compositional change to a polymer (e.g. substitution) and the structural change induced by polymer chain branching.


Keywords: English Application Note Molecular structure Molecular size Molecular weight OMNISEC 

GPC/SEC analysis of polylactic acid (PLA) and poly(lactic-co-glycolic acid) (PLGA) using OMNISEC Application Note (English)

In this application note, a selection of different PLA and PLGA polymer samples were analyzed on Malvern's OMNISEC GPC/SEC system, which combines multiple detectors to provide information about structure and solution properties.


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Upcoming webinar

From 0 to 60 – Novel polymer applications in the automotive industry
(September 3 2015)

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