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Size Exclusion Chromatography SEC also known as Gel Permeation Chromatography GPC or Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), or Gel Filtration Chromatography (GFC) is a chromatographic technique that separates dissolved molecules on the basis of their size by pumping them through specialized columns containing a microporous packing material.

As the sample is separated and elutes from the column, it can be characterized by a single concentration detector (Conventional Calibration) or series of detectors (Universal Calibration and Triple Detection).

This technique is used to characterize natural and synthetic polymers, biopolymers, proteins or nanoparticles.

When the Size Exclusion Chromatography separation is coupled with light scattering, viscometer and concentration detectors together (triple detection), it will provide a distribution of absolute molecular weight, molecular size, and intrinsic viscosity as well as information on macromolecular structure, conformation aggregation and branching.

Related products

Zetasizer µV Strap line: Modular size and molecular weight detector that can be added to any SEC system. Image
Measurement
Molecular size
Temperature range
2°C to 90°C
Particle size range
0.0003µm to 2µm
Dispersion type
Wet
Technology
Size Exclusion Chromatography, Dynamic Light Scattering
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Viscotek range The leading multi-detector GPC/SEC systems and detectors. Image
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Accuracy
Technology
Dilute Solution Viscometry, Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
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Malvern Biotherapeutic Characterization Seminar, Framingham, MA Seminar (English)

Learn More – Review theory behind dynamic light scattering (DLS), size exclusion chromatography, and static light scattering. Then see how it is applied to characterize biomolecules, nanoparticles, and emulsion materials. See More – Me...

Date:
May 6 2014
Location:
Sheraton Framingham Hotel and Conference Center, 1657 Worcester Rd, Framingham, Massachusetts, MA, USA, 01701
Language:
English

Keywords: English Zeta potential Dynamic Light Scattering Size Exclusion Chromatography USA Seminar Eastern Time Biological drug characterization and development 

Malvern Biotherapeutic Characterization Seminar - King of Prussia, PA Seminar (English)

Learn More – Review theory behind dynamic light scattering (DLS), size exclusion chromatography, and static light scattering. Then see how it is applied to characterize biomolecules, nanoparticles, and emulsion materials. See More – Me...

Date:
May 21 2014
Location:
Sheraton Valley Forge, 480 North Gulph Road, King of Prussia, Pennsylvania, PA, USA, 19406
Language:
English

Keywords: English Zeta potential Dynamic Light Scattering Size Exclusion Chromatography USA Seminar Eastern Time Biological drug characterization and development 

Columnas y Standares: Como hacer la correcta seleccion de las columnas y Standares para tu aplicacion de GPC/SEC Webinar - Recorded (Spanish)

Con una variedad de columnas para elegir, seleccionar las apropiadas asícomo los estándares para su aplicación GPC / SEC puede ser un reto. En este webinar, primero proporcionaremos una visión general de los tipos de columnas mas comunes ...

Product:
Viscotek range
Date recorded:
March 6 2014
Language:
Spanish

Keywords: Viscotek range Gel Permeation Chromatography Size Exclusion Chromatography Webinar - Recorded Mexico Time Spanish 

Investigating the Molecular Weight and Structure of Linear and Cross-Linked Hyaluronic Acid by SEC-MALS and SEC-Triple Detection Article (English)

This article demonstrates the application of a multi-angle light scattering (MALS) detector within a triple detector array to measure absolute molecular weight distribution and gain insight into molecular structure for an application involvin...


Keywords: English Article Viscotek range Molecular structure Molecular weight Static Light Scattering Size Exclusion Chromatography Cosmetics Personal care Consumer healthcare Polysaccharides 

From the applications laboratory: Using GPC/SEC to analyze elastomers Webinar - Recorded (English)

This webinar presents examples of GPC/SEC analysis of various elastomer chemistries and highlights the physical characterization information provided by this technique.

Product:
Viscotek range
Date recorded:
September 2 2013
Language:
English

Keywords: Polymer solutions English Viscotek range Molecular structure Molecular weight Gel Permeation Chromatography Size Exclusion Chromatography Polymers, plastics and rubbers Webinar - Recorded Greenwich Time 
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