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Size Exclusion Chromatography (SEC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.
When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.
By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:
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Gel Permeation Chromatography (GPC) also known as Size Exclusion Chromatography (SEC), is a very popular analytical tool for polymer characterization. Low molecular weight polymers such as polyols, epoxies and polycaprolactones (amongst others) are ...
Here we describe the features and benefits of the OMNISEC GPC/SEC system and explain how OMNISEC sets a new standard in molecular characterization.
GPC/SEC is mainly used for the characterization of macromolecules including synthetic and natural polymers (including polysaccharides) as well as proteins or RNA/DNA. This white paper provides an introduction to the technique in 30 minutes.
PLA, PLGA and their copolymers are biocompatible, biodegradable and used in a number of medical applications. However, analysis of this low molecular weight and low dn/dc sample pushes the sensitivity boundaries of all previous light scattering detec...
The properties of polymeric excipients can directly affect the clinical efficacy, safety, and quality of a finished pharmaceutical product and are routinely identified as critical quality attributes (CQAs). This article looks at how gel permeation/si...
(Webinar - Recorded)
How low can you go? New advances in the analysis of low molecular weight polymers by GPC/SEC
(August 25 2015)
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