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Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.
When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.
By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:
For polymer and protein characterization, in polymer, food, pharmaceutical and biopharmaceutical research, Malvern offers a range of SEC systems or individual SEC detectors, including SEC-MALS, to suit your application needs.
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A defining feature of polysaccharides, which are used widely as food additives and for certain medical applications, is that they are naturally occurring and consequently prone to inherent variability. In this article we discuss how advanced gel perm...
Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is a very popular analytical technique for characterizing natural and synthetic polymers. Malvern offers a range of multi-detector GPC systems for the advanced ch...
Significant advances have been made in analytical technology for the characterization and identification of particles present in pharmaceutical products. With these advances comes a tremendous amount of new data with which to characterize biologics, ...
Correctly targeting a drug product’s delivery location and defining its release profile are critical challenges and the subject of a great deal of research. The role of polymers in drug delivery applications is growing, with particular emphasis on ta...
The drug delivery polymer polyglutamic acid was conjugated with different amounts of Doxorubicin and characterized using advanced multi-detector GPC to measure drug content, molecular weight and structure
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