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Size Exclusion Chromatography (SEC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.
When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.
By using SEC to measure molecular weight and these other properties, scientists can characterize molecules such as proteins, synthetic polymers, and natural polymers such as polysaccharides.
For all of these molecules, these parameters relate very closely to their performance in countless applications. With this technique:
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Different samples of commercially available PLGA were analyzed to compare their absolute molecular weight from light scattering to those quoted with the product. Additionally, the Mark-Houwink plots of different examples containing different ratios o...
A review of the methods available for measuring the key characteristics of polymers focusing on the benefits and value of gel permeation/size exclusion chromatography (GPC/SEC). Much of the paper talks exclusively about polymers, however many of the ...
In this application note, we describe the analysis of a semi-purified protein mixture containing β-amylase. Step-by-step, we show how different properties of the proteins elicit a specific response in each detector and how the coupling this data can...
During this webinar, we introduces the latest innovations in SEC technology, and discuss how the new OMNISEC system will assist your protein characterization challenges. Focusing on applications, the webinar compares the capabilities of the system wi...
A series of dextran samples ranging in molecular weight from about 1– 650 kDa are characterized using OMNISEC. Structural comparisons to other polysaccharides, gum arabic and pectin are presented
(Webinar - Recorded)
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