Skip to left navigation
Skip to page content
Static light scattering (SLS) is a technique to measure absolute molecular weight using the relationship between the intensity of light scattered by a molecule and its molecular weight and size, as described by the Rayleigh theory. In the simplest terms, Rayleigh theory says that larger molecules scatter more light than smaller molecules from a given light source and that the intensity of the scattered light is proportional to the molecule’s molecular weight.
There are two ways to measure absolute molecular weight by SLS:
Batch measurement with cuvette based instruments, such as the Zetasizer series, is an ensemble technique. Therefore the result calculated is the weight average molecular weight of the entire sample measured.
However, the most common way of measuring absolute molecular weight is to add an SLS detector e.g. Low Angle Light Scattering LALS, Right Angle Light Scattering RALS or Multi Angle Light Scattering MALS to a GPC/SEC system. By combining SLS with the separation technique you can calculate the absolute molecular weight at any point in the eluting chromatogram and determining the molecular weight of any population in a mixed sample becomes possible.
Free access to exclusive content including webinars, presentations, application notes, technical notes, whitepapers and more. Enter your search term in the box and use the drop down boxes to filter the results.
The launch of Malvern Instruments’ Viscotek SEC-MALS 20 chromatography detector has triggered record downloads of one of the supporting white papers ‘Static light scattering technologies for GPC/SEC explained’, underlining the enthusiasm ...
The aim of this technical note is to show through a series of application examples, how the performance specifications of the Viscotek SEC-MALS 20 are derived.
This technical note decribes the technical advantages of the hardware inside the SEC-MALS 20 that place its performance above other types of MALS detector.
Molecular weight data from the Viscotek SEC-MALS 20 is combined with measurements of Rg and IV to study the structure of different polysaccharides. The differences between conformation and Mark-Houwink plots are also studied.
Sample Preparation for Molecular Weight measurements on the Zetasizer Nano and μV
10 Reasons that the zetasizer nano is chosen for scientific success
Sample measurements to demonstrate the specifications of the Viscotek SEC-MALS 20
Sample measurements performed to demonstrate Zetasizer performance
(Webinar - Recorded)
All information supplied within is correct at time of publication.
Malvern Instruments pursues a policy of continual improvement due to technical development. We therefore reserve the right to deviate from information, descriptions, and specifications in this publication without notice. Malvern Instruments shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance or use of this material.
Malvern and the 'hills' logo, Bohlin, Gemini, Insitec, ISys, Kinexus, Mastersizer, Morphologi, Rosand, SyNIRgi, Viscotek and Zetasizer, are International Trade Marks owned by Malvern Instruments Ltd.