Skip to left navigation

Skip to page content

Find us on...

Static light scattering (SLS) is a technique to measure absolute molecular weight using the relationship between the intensity of light scattered by a molecule and its molecular weight and size, as described by the Rayleigh theory. In the simplest terms, Rayleigh theory says that larger molecules scatter more light than smaller molecules from a given light source and that the intensity of the scattered light is proportional to the molecule’s molecular weight.

There are two ways to measure absolute molecular weight by SLS:

  1. Batch measurement using a cuvette
  2. In combination with a chromatography instrument.

Batch measurement with cuvette based instruments, such as the Zetasizer series, is an ensemble technique. Therefore the result calculated is the weight average molecular weight of the entire sample measured.

However, the most common way of measuring absolute molecular weight is to add an SLS detector e.g. Low Angle Light Scattering LALS, Right Angle Light Scattering RALS or Multi Angle Light Scattering MALS to a GPC/SEC system. By combining SLS with the separation technique you can calculate the absolute molecular weight at any point in the eluting chromatogram and determining the molecular weight of any population in a mixed sample becomes possible.

Related products

Viscotek range The leading multi-detector GPC/SEC systems and detectors. Image
Measurement
Molecular structure, Molecular weight, Molecular size, Protein aggregation, Rheology and viscosity
Accuracy
Technology
Dilute Solution Viscometry, Size Exclusion Chromatography, Gel Permeation Chromatography, Static Light Scattering
More information
Zetasizer range The worlds most widely used system for nanoparticle, colloid and protein size, zeta potential and molecular weight measurements. Image
Measurement
Molecular size, Particle size, Molecular weight, Zeta potential
Temperature range
Particle size range
Dispersion type
Wet
Technology
Dynamic Light Scattering, Static Light Scattering, Size Exclusion Chromatography, Electrophoretic Light Scattering
More information
 
 

Resource center search

Free access to exclusive content including webinars, presentations, application notes, technical notes, whitepapers and more. Enter your search term in the box and use the drop down boxes to filter the results.

Reset filters
Items shown: 5, 10, 20, ALL
Sort by: Date or Alphabetical
Previous page Showing results 1 - 5 of 83 Next page
PEGylated proteins: Optimizing conjugation reactions using triple detection SEC Application Note (English)

In this application note, interferon α-5 was conjugated with two different PEG molecules in different reactions. The individual protein and PEG molecules, as well as the PEGylated conjugates are characterized by SEC and their composition,...


Keywords: English Application Note Molecular size Molecular weight Static Light Scattering Size Exclusion Chromatography Protein aggregation Proteins Biological drug characterization and development Viscotek TDAmax Viscotek SEC-MALS Viscotek UV-PDA Detector (2600) 

Overcoming the negative effect of protein structure on molecular weight measurement by Size Exclusion Chromatography – Two protein case studies with Anthrolysin and Bcl-2 Application Note (English)

A description of how advanced GPC/SEC detection can overcome inaccuracy in the measurement of molecular weight caused by deviation in the structure of the sample protein from that of the protein standards or interaction with the chromatography column...


Keywords: English Application Note Viscotek range Molecular structure Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Viruses and vaccines Protein aggregation Proteins 



Improved understanding of the thermal stability of a monoclonal antibody and its modified version by use of DLS/Raman spectroscopy Whitepaper (English)

Use of DLS combined with Raman spectroscopy simultaneously monitors both the colloidal and conformational stability of a template monoclonal antibody (mAb) and a modified monoclonal antibody (mmAb) and provides significant insights into the mechanist...


Keywords: English Whitepaper Molecular structure Molecular size Dynamic Light Scattering Electrophoretic Light Scattering Raman spectroscopy Static Light Scattering Protein aggregation Proteins Biological drug characterization and development Bioformulation development Zetasizer Helix 
Previous page Showing results 1 - 5 of 83 Next page

Glossary

Contact sales Register now