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Static light scattering (SLS) is a technique to measure absolute molecular weight using the relationship between the intensity of light scattered by a molecule and its molecular weight and size, as described by the Rayleigh theory. In the simplest terms, Rayleigh theory says that larger molecules scatter more light than smaller molecules from a given light source and that the intensity of the scattered light is proportional to the molecule’s molecular weight.

There are two ways to measure absolute molecular weight by SLS:

  1. Batch measurement using a cuvette
  2. In combination with a chromatography instrument.

Batch measurement with cuvette based instruments, such as the Zetasizer series, is an ensemble technique. Therefore the result calculated is the weight average molecular weight of the entire sample measured.

However, the most common way of measuring absolute molecular weight is to add an SLS detector e.g. Low Angle Light Scattering LALS, Right Angle Light Scattering RALS or Multi Angle Light Scattering MALS to a GPC/SEC system. By combining SLS with the separation technique you can calculate the absolute molecular weight at any point in the eluting chromatogram and determining the molecular weight of any population in a mixed sample becomes possible.

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Key considerations when purchasing an advanced GPC/SEC system Whitepaper (English)

Investing in a new GPC/SEC system is a big decision with many factors to consider. This white paper lists the key items that you should think about when buying a new system and discusses the reasons for their importance.  Finally, it discusses what M...

What is the Derived Count Rate? FAQ

The derived count rate is the normalized count rate taking into account the attenuation factor used

Optimizing drug delivery polymer conjugate levels using Advanced Multi-Detector GPC Application Note (English)

The drug delivery polymer polyglutamic acid was conjugated with different amounts of Doxorubicin and characterized using advanced multi-detector GPC to measure drug content, molecular weight and structure

Demo at your desk: Zetasizer Webinar - Recorded (English)

This online demo is designed to provide you with a virtual hands-on introduction to the Zetasizer Nano. You will be able to take a look at the Zetasizer Nano and some of its accessories via webcam. We will screen-share the software with you so that y...

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Date recorded:
February 25 2016

Orthogonal and complementary nanoparticle characterization techniques Whitepaper (English)

How can I trust my data? Is it affected in any way by the measurement technology, or  by the user through sample preparation or the analysis parameters employed? Independent and orthogonal measurement modalities should be used to provide measurement ...

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