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Static light scattering (SLS) is a technique to measure absolute molecular weight using the relationship between the intensity of light scattered by a molecule and its molecular weight and size, as described by the Rayleigh theory. In the simplest terms, Rayleigh theory says that larger molecules scatter more light than smaller molecules from a given light source and that the intensity of the scattered light is proportional to the molecule’s molecular weight.
There are two ways to measure absolute molecular weight by SLS:
Batch measurement with cuvette based instruments, such as the Zetasizer series, is an ensemble technique. Therefore the result calculated is the weight average molecular weight of the entire sample measured.
However, the most common way of measuring absolute molecular weight is to add an SLS detector e.g. Low Angle Light Scattering LALS, Right Angle Light Scattering RALS or Multi Angle Light Scattering MALS to a GPC/SEC system. By combining SLS with the separation technique you can calculate the absolute molecular weight at any point in the eluting chromatogram and determining the molecular weight of any population in a mixed sample becomes possible.
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Use of static and dynamic light scattering to characterize the stability of lysozyme in the presence and absence of arginine. SLS is used to assess agregation temperature, while DLS is used to measure the various particle sizes present in the two for...
The launch of Malvern Instruments’ Viscotek SEC-MALS 20 chromatography detector has triggered record downloads of one of the supporting white papers ‘Static light scattering technologies for GPC/SEC explained’, underlining the enthusiasm ...
This article demonstrates the application of a multi-angle light scattering (MALS) detector within a triple detector array to measure absolute molecular weight distribution and gain insight into molecular structure for an application involvin...
The aim of this technical note is to show through a series of application examples, how the performance specifications of the Viscotek SEC-MALS 20 are derived.
This technical note decribes the technical advantages of the hardware inside the SEC-MALS 20 that place its performance above other types of MALS detector.
Comparison of SEC-LS and DLS capabilities in the detection and quantification of large protein aggregates
Assessing the quality of proteins using light scattering techniques
The use of Zeta potential to investigate the controlled self-assembly of tooth enamel
Investigating the Molecular Weight and Structure of Linear and Cross-Linked Hyaluronic Acid by SEC-MALS and SEC-Triple Detection
10 Reasons that the zetasizer nano is chosen for scientific success
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