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Static light scattering (SLS) is a technique to measure absolute molecular weight using the relationship between the intensity of light scattered by a molecule and its molecular weight and size, as described by the Rayleigh theory. In the simplest terms, Rayleigh theory says that larger molecules scatter more light than smaller molecules from a given light source and that the intensity of the scattered light is proportional to the molecule’s molecular weight.

There are two ways to measure absolute molecular weight by SLS:

  1. Batch measurement using a cuvette
  2. In combination with a chromatography instrument.

Batch measurement with cuvette based instruments, such as the Zetasizer series, is an ensemble technique. Therefore the result calculated is the weight average molecular weight of the entire sample measured.

However, the most common way of measuring absolute molecular weight is to add an SLS detector e.g. Low Angle Light Scattering LALS, Right Angle Light Scattering RALS or Multi Angle Light Scattering MALS to a GPC/SEC system. By combining SLS with the separation technique you can calculate the absolute molecular weight at any point in the eluting chromatogram and determining the molecular weight of any population in a mixed sample becomes possible.

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Investigating the effects of excipients on the earliest stages of aggregation - Aggregation inhibition by arginine Application Note (English)

Use of static and dynamic light scattering to characterize the stability of lysozyme in the presence and absence of arginine. SLS is used to assess agregation temperature, while DLS is used to measure the various particle sizes present in the two for...


Keywords: English Application Note Molecular size Particle size Zeta potential Dynamic Light Scattering Static Light Scattering Zetasizer Nano range Protein aggregation Proteins Protein mobility Biological drug characterization and development Bio-formulation development 

All angles covered. Press Release (English)

The launch of Malvern Instruments’ Viscotek SEC-MALS 20 chromatography detector has triggered record downloads of one of the supporting white papers ‘Static light scattering technologies for GPC/SEC explained’, underlining the enthusiasm ...


Keywords: English Static Light Scattering Press Release Viscotek SEC-MALS 

Investigating the Molecular Weight and Structure of Linear and Cross-Linked Hyaluronic Acid by SEC-MALS and SEC-Triple Detection Article (English)

This article demonstrates the application of a multi-angle light scattering (MALS) detector within a triple detector array to measure absolute molecular weight distribution and gain insight into molecular structure for an application involvin...


Keywords: English Article Viscotek range Molecular structure Molecular weight Static Light Scattering Size Exclusion Chromatography Cosmetics Personal care Consumer healthcare Polysaccharides 

Sample measurements to demonstrate the specifications of the Viscotek SEC-MALS 20 Technical Note (English)

The aim of this technical note is to show through a series of application examples, how the performance specifications of the Viscotek SEC-MALS 20 are derived.


Keywords: English Technical Note Viscotek range Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Viscotek SEC-MALS 

Technical benefits of the Viscotek SEC-MALS 20 Technical Note (English)

This technical note decribes the technical advantages of the hardware inside the SEC-MALS 20 that place its performance above other types of MALS detector.


Keywords: English Technical Note Viscotek range Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Viscotek SEC-MALS 
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