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Static light scattering (SLS) is a technique to measure absolute molecular weight using the relationship between the intensity of light scattered by a molecule and its molecular weight and size, as described by the Rayleigh theory. In the simplest terms, Rayleigh theory says that larger molecules scatter more light than smaller molecules from a given light source and that the intensity of the scattered light is proportional to the molecule’s molecular weight.

There are two ways to measure absolute molecular weight by SLS:

  1. Batch measurement using a cuvette
  2. In combination with a chromatography instrument.

Batch measurement with cuvette based instruments, such as the Zetasizer series, is an ensemble technique. Therefore the result calculated is the weight average molecular weight of the entire sample measured.

However, the most common way of measuring absolute molecular weight is to add an SLS detector e.g. Low Angle Light Scattering LALS, Right Angle Light Scattering RALS or Multi Angle Light Scattering MALS to a GPC/SEC system. By combining SLS with the separation technique you can calculate the absolute molecular weight at any point in the eluting chromatogram and determining the molecular weight of any population in a mixed sample becomes possible.

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Powerful protein SEC made simple! Webinar - Live (English)

This webinar will discuss how Malvern’s protein SEC measurement capabilities within the Viscotek range have developed and improved in the last year. We will also introduce the new features that are included in the latest release of OmniSEC, i...

Product:
Viscotek range,Viscotek RImax,Viscotek DSV,Viscotek 270max,Viscotek TDAmax,Viscotek SEC-MALS
Date:
September 30 2014
Language:
English

Keywords: English Viscotek range Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Webinar - Live Eastern Time Protein aggregation Proteins Biopharmaceutical development Viscotek 270max Viscotek DSV Viscotek RImax Viscotek TDAmax Viscotek SEC-MALS 

Characterization of Protein-Protein Interactions using triple detection size exclusion chromatography (SEC-TD) Application Note (English)

In this application note, triple detection size exclusion chromatography (SEC-TD) is used to understand the ability of proteins to form ordered, structured complexes. The controlling mechanism behind these protein complexes is fundamental to ...


Keywords: English Application Note Molecular size Molecular weight Static Light Scattering Size Exclusion Chromatography Viruses and vaccines Proteins Biological drug characterization and development Viscotek TDAmax Viscotek TDA 305 

PEGylated proteins: Optimizing conjugation reactions using triple detection SEC Application Note (English)

In this application note, interferon α-5 was conjugated with two different PEG molecules in different reactions. The individual protein and PEG molecules, as well as the PEGylated conjugates are characterized by SEC and their composition,...


Keywords: English Application Note Molecular size Molecular weight Static Light Scattering Size Exclusion Chromatography Protein aggregation Proteins Biological drug characterization and development Viscotek TDAmax Viscotek SEC-MALS Viscotek UV-PDA Detector (2600) 

The Science of Beauty - Characterizing Hyaluronic Acid - the molecule behind anti-aging creams Webinar - Recorded (English)

Hyaluronic acid (HA) is a natural polysaccharide, that plays an important role in tissues, such as skin, tendons, muscles, and cartilage. As well as medical applications, It finds wide-spread use in cosmetics, particularly skin creams. In...

Product:
Viscotek TDAmax,Viscotek SEC-MALS,Zetasizer Nano ZS,Zetasizer Nano ZSP
Date recorded:
June 25 2014
Language:
English

Keywords: Polymer solutions English Molecular size Molecular weight Dynamic Light Scattering Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Cosmetics Personal care Specialty and novel polymers Webinar - Recorded Eastern Time Zetasizer Nano ZS Zetasizer Nano ZSP Microrheology Viscotek TDAmax Viscotek SEC-MALS 

Polymer solution characterization part 3: Branching out! Intrinsically useful views on polymer structure Webinar - Recorded (English)

Most scientists can easily understand polymer molecular weight differences but often intrinsic viscosity, a harder concept to grasp, is a more useful measure of a polymer in solution. This webinar describes in simple terms what intrinsic ...

Product:
Viscotek TDAmax,Viscotek 270max,Viscotek RImax,Viscotek TDA 305,Viscotek SEC-MALS,Viscotek RImax
Date recorded:
June 24 2014
Language:
English

Keywords: Polymer solutions English Molecular structure Molecular size Molecular weight Gel Permeation Chromatography Static Light Scattering Size Exclusion Chromatography Food additives Bulk (commodity) polymers Rubbers and elastomers Polymers from renewable sources Polysaccharides Webinar - Recorded Eastern Time Proteins Viscotek 270max Viscotek RImax Viscotek TDAmax Viscotek SEC-MALS Viscotek TDA 305 
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Powerful protein SEC made simple!
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