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Static light scattering (SLS) is a technique to measure absolute molecular weight using the relationship between the intensity of light scattered by a molecule and its molecular weight and size, as described by the Rayleigh theory. In the simplest terms, Rayleigh theory says that larger molecules scatter more light than smaller molecules from a given light source and that the intensity of the scattered light is proportional to the molecule’s molecular weight.
There are two ways to measure absolute molecular weight by SLS:
Batch measurement with cuvette based instruments, such as the Zetasizer series, is an ensemble technique. Therefore the result calculated is the weight average molecular weight of the entire sample measured.
However, the most common way of measuring absolute molecular weight is to add an SLS detector e.g. Low Angle Light Scattering LALS, Right Angle Light Scattering RALS or Multi Angle Light Scattering MALS to a GPC/SEC system. By combining SLS with the separation technique you can calculate the absolute molecular weight at any point in the eluting chromatogram and determining the molecular weight of any population in a mixed sample becomes possible.
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A series of dextran samples ranging in molecular weight from about 1– 650 kDa are characterized using OMNISEC. Structural comparisons to other polysaccharides, gum arabic and pectin are presented
State-of-the-art GPC/SEC systems, such as OMNISEC, offer the sensitivity required to precisely evaluate different grades of polymer-based food additive and to make an optimal choice in formulation
Using a light scattering detector as part of a multi-detector Size Exclusion Chromatography system provides the accurate molecular weight (MW) data needed to assess the activity, safety and clinical efficacy of therapeutic proteins.
Realize the maximum potential of your polymer using unsurpassed characterization of molecular weight and structure using OMNISEC – our latest gel permeation chromatography / size exclusion chromatography system.
Dr. Mark Pothecary, Product Manager, ...
In this application note, a selection of different PLA and PLGA polymer samples were analyzed on Malvern's OMNISEC GPC/SEC system, which combines multiple detectors to provide information about structure and solution properties.
(Webinar - Recorded)
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