More information:
	Protein solutions for batch and SEC
	Protein characterization overview
	What is the hydrodynamic radius
	About Zeta Potential
	Malvern Zetasizer Nano for proteins
Case studies :
	Size examples for common proteins
	Protein sensitivity calculator
	Combining chromatography and dynamic light scattering

Enhanced Resolution Protein Characterization

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Absolute SEC

Absolute Size Exclusion Chromatography

Combining SEC with the Zetasizer Nano as a dynamic light scattering detector

Size Exclusion Chromatography (SEC) is a common technique in biochemistry laboratories and routinely used for purification, identification, and quantification of protein mixtures. Dynamic light scattering (DLS) is traditionally used for pre-column size & polydispersity measurements, along with aggregate quantification.
Aside from the enhanced sensitivity, a big advantage of dynamic light scattering coupled with SEC is the ability to perform Absolute SEC (ASEC), or size exclusion chromatography in the absence of costly and time consuming column calibration. Dynamic light scattering provides a direct measure of the protein size, as opposed to the SEC derived size extracted from a calibration curve. In addition, size vs. mass relationships for globular proteins and polymeric systems are well known, and can be used to calculate the molecular mass from the dynamic light scattering measured hydrodynamic radius.

Samples are measured directly after elution from a chromatography column
The scattering intensity, hydrodynamic size, and molecular weight of the protein are displayed in real time, under flow
Absolute method requires no column calibration or secondary detectors for molecular weight determination
Size distribution resolution is enhanced through combination with chromatography

In this section


	Protein characterization overview Dynamic light scattering offers additional information about your protein sample. The non-invasive protein characterization technique is fast, accurate, simple to perform, and you can recover the sample when used in batch mode!


	Solutions for protein characterization applications Determination of protein structure is a priority for understanding the role and function of many proteins. One indicator of structure is the size of the molecule. This rapid method is a convenient route to obtain an indication of the quaternary structure of a protein. Many proteins show profound changes in different solution conditions, with the most extreme structure change being protein denaturation. Similar structure changes may happen as a function of time, pH, ionic strength and changes in various other solution conditions. Dynamic light scattering is an ideal protein characterization tool to quickly assess the condition of the molecule in solution under such a range of conditions.


	Malvern equipment for protein characterization and automation Find out more about the Zetasizer Nano: performance, sensitivity, minimum volume, temperature range, software. The Zetasizer Nano can be used in conjunction with the MPT-2 titrator for automated, unattended screens of different solvent conditions (pH, salt).