Protein characterization- size and molecular weight in batch mode and by SEC

Proteins form the major building blocks of life. Many recent pharmaceutical advances (e.g. therapeutics, antibodies) are based on detailed knowledge and characterization of the underlying proteins involved.

How do you know if the protein is well-behaved in the buffer supplied?

What is the true form of the protein in solution?

Achieve the best possible competitive advantage by:

• Optimizing protein crystallization conditions
  
  
• Measuring at true concentrations used in research environment
  
  
• Determining absolute molecular weight and polydispersity
  
  
• Assessing the importance of the second virial coefficient
  
  
• Characterizing protein homogeneity by dynamic light scattering
  
  
• Combining chromatography with dynamic light scattering to determine size and a rapid estimation of molecular weight
  

In this section

	Protein characterization overview Dynamic light scattering offers additional information about your protein sample. The non-invasive protein characterization technique is fast, accurate, simple to perform, and you can recover the sample!
	Solutions for protein characterization applications Determination of protein structure is a priority for understanding the role and function of many proteins. One indicator of structure is the size of the molecule. This rapid method is a convenient route to obtain an indication of the quaternary structure of a protein. Many proteins show profound changes in different solution conditions, with the most extreme structure change being protein denaturation. Similar structure changes may happen as a function of time, pH, ionic strength and changes in various other solution conditions. Dynamic light scattering is an ideal protein characterization tool to quickly assess the condition of the molecule in solution under such a range of conditions.
	Malvern equipment for protein characterization and automation Find out more about the Zetasizer Nano: performance, sensitivity, minimum volume, temperature range, soft ware. The Zetasizer Nano can be used in conjunction with the MPT-2 titrator for automated, unattended screens of different solvent conditions (pH, salt).