I want to measure molecular weight

Use the Zetasizer Nano to make batch measurements in cuvettes, and also use as a chromatography detector (ASEC)

The molecular weight of biological materials and polymers can be determined from both static light scattering (SLS) and dynamic light scattering (DLS) experiments using the Zetasizer Nano. In addition the molecular size of very small molecules in solution can be measured using the Malvern Zetasizer Nano, both as a batch measurement in cuvettes, and with the use of a flow cell, as a detector at the end of a chromatography column


Cuvette mode
Molecular weight measured by Static Light Scattering - The sample concentration is required, as well as the optical 'contrast' of the material, known as the dn/dc. The molecular weight can also be obtained from a Dynamic Light Scattering measurement of the sample, which is simpler and faster to perform. The concentration and optical properties of the sample are not required, and the accuracy for protein measurements is comparable.


Chromatography detector mode
The Zetasizer can be used as a chromatography detector by using a flow cell to connect the system to the end of an SEC/GPC set-up.
The size of material measured as it is eluted from the column is an absolute value, hence the term Absolute Size Exclusion Chromatography (ASEC).
This size is readily converted to molecular weight with excellent accuracy using well accepted models.
External detector signals can by input into the Zetasizer to enable simultaneous plotting and storage of data.

I want to measure:

	Molecular weight measurement using dynamic light scattering (DLS): The Zetasizer Nano S and Nano ZS can be used to measure the absolute size of molecules, then can be interpreted as a molecular weight distribution. The calculation uses the Mark-Houwinck equations, which rely on a calibration technique where examples of the same material of known molecular weight are available. These are used to recalculate the size axis from a size distribution experiment in terms of molecular weight. Sizes can be measured of molecules greater than 1,000 Da.
	Molecular weight measurement using a single angle spectrometer: The Malvern Zetasizer Nano S and ZS has been designed to use the intensity of light scattered at a single angle over a range of sample concentrations to determine absolute molecular weight. This light scattering technique is applicable to globular polymers such as proteins up to 20,000kDa and random coil polymers up to 500kDa.
	Molecular size measurement using dynamic light scattering: The Malvern Zetasizer Nano S uses dynamic light scattering to measure size, and is sensitive enough to measure the size of molecules below 1000 Da. The molecular size measurement does not require prior knowledge of the material dn/dc value, and can also provide size distributions, which may be important for the determination of aggregates.