Light Scattering Detector

The powerful light scattering detector provides two separate measurement points.  The unique 7° Low Angle (LALS) detector measures the absolute molecular weight of even the largest macromolecules directly without need of data fitting, extrapolation or correction.

To complement the LALS, and to enable the highest sensitivity measurements for small molecules such as proteins, the 90° Right Angle (RALS) detector is employed. The intelligent OmniSEC software collects data from both detectors and uses the appropriate signal for the sample being analysed. This maximises the signal-to-noise and the accuracy of the molecular weight determination, even when a sample contains complex mixtures of large and small molecules. A low cell volume of just 18μL, coupled with unmatched optical efficiency, ensure low band broadening and high sensitivity.

More information

	Featured presentations and application notes:
	Application communication: Protein and polymer molecular size by GPC/SEC The main goal of most Gel Permeation/Size Exclusion Chromatography (GPC/SEC) experiments is to determine the molecular weight distribution (MWD) of the sample or compare molecular weights (MW) of several samples.
	On-demand presentation: Basic Triple Detection Chromatography Size Exclusion Chromatography (SEC), also called Gel Permeation Chromatography (GPC) is a separation technique used to analyze the molecular weight and molecular weight distribution. The technique is used extensively for synthetic polymers characterization. Aqueous chromatography is trickier due to the electrostatic interaction in between the column packing material and the sample to be separated. Triple detection allows for absolute measurements of the molecular weights and distribution without column calibration, alleviating the problems caused by electrostatic interaction.
	More application notes and online presentations are available to search for FREE in the Malvern Application Library and Presentations Database (Requires Free registration).