Viscotek TDAmax

Overview

The TDAmax consists of three components – the GPCmax integrated solvent and sample delivery module, the TDA 305 Triple Detector Array, and the OmniSEC software. The TDA 305 incorporates RI and, Light Scattering detectors, as well as a viscometer and optional UV detector. Both the integrated detectors and the chromatography columns are fully temperature controlled up to 80°C in an oven to ensure optimum detector stability. In addition, the detectors are connected in series to ensure maximum sensitivity.

• Absolute molecular weight of small polymers, protein stability and protein aggregation using Right angle light scattering (RALS)
• Direct output of absolute molecular weight of polymers without extrapolation using Low angle light scattering (LALS)
• Protein and polymer structure (including branching) information using intrinsic viscosity detector
• Molecular size in terms of hydrodynamic diameter (Rh) and the radius of gyration (Rg)
• Copolymer composition using photo-diode array UV detector
• Autosampler incorporated to increase throughput and improve repeatability
• Advanced GPC/SEC software capable of going from data to results in just two clicks
• 3D absorption plot for chemical identification of each component
• 21 CFR part 11 compliant
• Full suite of calibration and GPC/SEC calculation types

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How it works

The GPCmax contains an integrated pump, autosampler and degasser. Mobile phase, stored in solvent bottles placed on top of the instrument, enters the dual piston pump after passing through the eluent sensor. This will automatically stop the flow if the solvent is exhausted mid-run. An inline degasser then removes dissolved gases from the mobile phase, which improves the baseline on any of the detectors that may be connected. The pump is compatible with any solvent, even acidic buffers.

The autosampler inside the GPCmax can hold up to 120 vials, which can either be heated, or cooled to prevent degradation of the samples over time. The Autosampler provides the system with variable, reliable, accurate and reproducible injection volumes, enabling comprehensive macromolecular characterizations to be made with confidence.

Mobile phase (and sample) enters the TDA and passes through the GPC/SEC chromatography columns. The columns are maintained at the same temperature as the detectors. After eluting from the column, the dissolved polymer molecules, now separated by size, pass through the UV detector (if present), followed by the light scattering and RI detectors. Finally, the mobile phase passes through the viscometer before going to waste.

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Specification

Complete GPC/SEC system including Conventional, Universal, Light Scattering, Triple, Copolymer, Protein specific, and FIPA calculation modules

Output of Mn, Mw, Mz, Mp, Mw/Mn, IV [η], Rh, Rg, Branching Number, Branching Frequency , Weight Fraction, Concentration, MH-a, MH-k, dn/dc, dA/dc. A2

System

GPCmax:

Pump flow rate:

0.01 to 9.99 mL/min, programmable soft start

Pulsation:

Less than 1%, measured with viscometer

Degasser:

Two channels, < 0.5ppm oxygen at ) 0.5mL/min, eluent
sensor to stop pump when eluent runs out

Dimensions:

55cm x 53cm x 38cm (W x D x H)

Weight:

37kg

Autosampler

Capacity:

120 vials, programmable sequence

Injection volume:

Variable 20 to 150µL

Reproducibility:

Better than 0.5%

Carryover:

Less than 0.1%, depending on wash program

Cooling option:

4°C to 40°C, limited to 60 vials

Heated tray option

Up to 65°C limited to 60 vials heated, 60 vials unheated

Detector:

TDAmax

Temperature range:

Ambient to 80°C, all detector cells and columns

Column capacity:

Up to 5 x 30cm columns

Interface:

6 channel digital to OmniSEC software,
2 analogue inputs RI and UV/LS

Dimensions:

41cm x 54cm x 28cm (W x D x H)

Weight

30Kg

Detector 1:

Light scattering

Measurement principle:

RALS 90° angle, LALS 7° angle

Light source:

3mW laser diode, 670nm, temperature controlled

Cell volume:

18µL

Data rate:

100Hz collection, 5Hz to OmniSEC

Detector 2:

Refractive index

Measurement principle:

Deflection

Light source:

LED

Cell volume:

12µL

Data rate:

100Hz collection, 5Hz to OmniSEC

Detector 3:

Viscometer

Measurement principle:

4 capillary differential Wheatstone bridge configuration

Transducers:

Digital Inert Transducers with over-pressure protection

Cell volume:

18µL

Data rate:

100Hz collection, 5Hz to OmniSEC

Detector 4 (option):

UV, Single wavelength programmable

Wavelength:

190 to 740nm

Light source

Deuterium, Tungsten halogen optional

Cell volume:

10µL

Data rate:

Analog, A-to-D with 5Hz to OmniSEC

Detector 5 (option):

UV-PDA

Wavelength:

190 to 500nm or 710nm on 256 diode array

Light source

Deuterium (190 – 500nm), Tungsten halogen (430 – 710nm)

Cell volume:

10µL

Data rate:

256 channels with 1Hz to OmniSEC

Laser safety:

Class 1

Operating environment:

Temperature:

15°C – 40°C

Humidity:

35% - 80% non-condensing

Software:

OmniSEC, 6 channels at 5Hz, unlimited acquisition time

Minimum computer specification:

100 GB HD, 2GB RAM, 1.8 GHz processor,
Graphics 1024x768, 16-bit color depth

Operating platforms:

Windows XP or higher

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Components

Malvern - Viscotek accessories include all the components to meet the requirements of your application

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Support

When you purchase a Malvern product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Some customers just need to ask an occasional question or to download a software upgrade, whilst others require a comprehensive support contract with priority call out and fixed cost of ownership. Whatever your situation Malvern will provide the right support for you.

Help Desk

• Telephone and email support.
• Operators who take responsibility for your call and ensure your query is answered.

Training

• On Site training courses

• Classroom training
• E-learning

Resource Centre

• Application notes
• Technical notes
• On demand presentations
• Live webinars

Software Downloads

• A simple mechanism for receiving updates and new features

Platinum and Gold Service Contracts:

• Protection from the cost of downtime
• No time lost in obtaining help when you need it
• Fixed cost of ownership

Bronze Service Agreements:

• Protect the value of your investment
• Reduce the risk of downtime
• Protect the validity of your data

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