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Molecular weight in 60 seconds |
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The molecular weight of an unknown protein is quickly obtainable from a dynamic light scattering (DLS) measurement. An empirical size-mass relationship produces excellent agreement for a wide range of proteins. The example of insulin shows the use of this relation. When an equilibrium exists between monomer and higher oligomeric state then this technique can be used as a fast estimate of the ratio of the two contributions. Several additional commonly encountered proteins are also presented.
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