Protein melting Point

Proteins are charged biomolecules that can fold into compact structures that are very sensitive to solution conditions. As an extreme case, thermal denaturation can lead to irreversible loss of structure and function of the molecule. Thermal denaturation typically leads to an increase in size that can be monitored by dynamic light scattering. At the protein melting point, a marked increase in hydrodynamic size and scattering intensity is observed. This protein melting point temperature is indicative of the thermal stability of a protein. Modifications (such as glycosylation) can influence the stability and are easily observed using dynamic light scattering.

Type:	Dynamic Light Scattering (PCS)
Duration:	3 minutes
Language:

Search for more Multimedia Presentations: