Measuring absolute molecular weight

Static light scattering is the study of the average scattering intensity as a function of angle and concentration. For small scattering particles the absolute molecular weight (MW) and the second virial coefficient (A2) can be obtained from a Debye plot, by recording the scattering intensity at different sample concentrations. Common proteins such as Interferon Gamma, bovine serum albumin (BSA), Ovalbumin, or lysozyme show absolute molecular weight in agreement with the expected mass spectroscopy results. Additional information is derived from the second virial coefficient, a parameter describing the interaction strength between the molecule and the solvent.

Type:	Dynamic Light Scattering (PCS)
Duration:	5 minutes
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