Oligomer & Quarternary Structure

Dynamic light scattering may be used to study the aggregation process. The term ‘aggregates’ can encompass oligomers, assembly or agglomeration. Typically, the molecular weight is measured using static light scattering. A recent, more rapid approach is the estimate from dynamic light scattering using a calibration technique. This method, for example, shows a clear difference between insulin in dimeric form at pH2 and a preparation in physiological conditions. The ‘active’ hexamer is significantly increased in size compared to the inactive dimer. Bovine serum albumin (BSA) is typically present as an equilibrium of dimer/monomer and Dynamic Light Scattering is used as a first estimate of the ratio of the two contributions.

Type:	Dynamic Light Scattering (PCS)
Duration:	4 minutes
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