Malvern Viscosizer TD is an automated biophysical characterization tool utilizing Taylor Dispersion Analysis and providing ultra-low volume, solution-based molecular size and stability measurement capabilities, combined with Poiseuille flow for relative viscosity assessment. Viscosizer TD offers biopharmaceutical researchers an orthogonal technique for conformational stability analysis, which is unrivaled in extending into label-free measurements in highly complex solutions, and encompasses a critical size range from small molecules through to protein-based systems.
Viscosizer TD is unique in using Taylor Dispersion Analysis with UV area imaging in microcapillary flow to enable ultra-low volume biophysical characterization of target biomolecules in solution.
Taylor Dispersion Analysis is an inherently simple experimental method, whereby a nanoliter volume sample pulse is injected into a laminar flow of matched buffer. Analysis of the time-evolved concentration profile (or Taylorgram) from UV absorption allows the molecular diffusion coefficient and hence hydrodynamic radius of solute molecules to be determined.
Viscosizer TD uses fully automated methodology to analyze stability of small molecules, peptides and proteins, and samples with mixtures of these species, even in the presence of excipients and surfactants that can be the source of measurement interference in other techniques.
Microcapillary flow methodology also allows complementary relative viscosity measurement on the same sample using Poiseuille’s law.
Viscosizer TD is a biophysical characterization tool that provides unique measurement capabilities for researchers assessing conformational stability, self-association and solubility of target biomolecules in complex solutions. Key application areas include: